Mycobiology

  • 제37권4호
  • /
  • Pages.317-319
  • /
  • 2009
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Development of Specific Primer for Tricholoma matsutake

  • Kim, Jang-Han (Department of Life Science, Graduate School, Dongguk University) ;
  • Han, Yeong-Hwan (Department of Life Science, Dongguk University)
  • 발행 : 2009.12.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

키워드

참고문헌

  1. Chapela, I. H. and Garbelotto, M. 2004. Phylogeography and evolution in matsutake and close allies inferred by analyses of ITS sequences and AFLPs. Mycologia 96:730-741 https://doi.org/10.2307/3762107
  2. Dunstan, W. A., Dell, B., Malajczuk, N. and Iwase, K. 2000. Detection of the ectomycorrhizal fungus Tricholoma matsutake and some related species with specific ITS primers. Mycoscience 41:33-37 https://doi.org/10.1007/BF02464383
  3. Kikuch, K., Matsushita, N., Guerin-Lauette, A., Ohta, A. and Suzuki, K. 2000. Detection of Tricholoma matsutake by specific primers. Mycol. Res. 104:1427-1430 https://doi.org/10.1017/S0953756200002653
  4. Lee, J. S., Noh, E. W., Choi, Y. I., Lee, W. Y. and Kah, K. H. 2004. Development of nuclear ribosomal ITS DNA marker for Tricholoma matsutake. J. Kor. For. Soc. 93:121-127
  5. Lee, S. S. and Hong, S. W. 1998. The 18s rDNA sequences of the basidiocarps of Tricholoma matsutake in Korea. Kor. J. Mycol. 26:256-264
  6. Lee, S. S., Hong, S. W., Chung, H., Sung, C. K., Kim, J. H., Ka, K. H. and Kim, H. J. 1999. The specific probes confirming the genomic DNA of Tricholoma matsutake in Korea. Kor. J. Mycol. 27:20-26
  7. Lian, C., Hogetsu, T., Matsushita, N., Guerin-Laguette, A., Suzuki, K. and Yamada, A. 2003. Development of microsatelite markers from an ectomycorrhizal fungus, Tricholoma matsutake by an ISSR-suppression-PCR method. Mycorrhiza 13:27-31 https://doi.org/10.1007/s00572-002-0193-6
  8. Min, E. G. and Han, Y. H. 2000. Characteristics of extracellular $\beta$-glucosidase in Tricholoma matsutake. Korean J. Biotechnol. Bioeng. 15:9-13
  9. Raeder, U. and Broda, P. 1985. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1:17-20 https://doi.org/10.1111/j.1472-765X.1985.tb01479.x
  10. Srivastava, A. K. and Schlessinger, D. 1991. Structure and organization of ribosomal DNA. Biochimie 73:631-638 https://doi.org/10.1016/0300-9084(91)90042-Y
  11. Thompson, J. D. Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice. Nucleic Acid Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673

피인용 문헌

  1. Diversity and effect of Trichoderma isolated from the roots of Pinus densiflora within the fairy ring of pine mushroom (Tricholoma matsutake) vol.13, pp.11, 2018, https://doi.org/10.1371/journal.pone.0205900