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Development of Specific Primer for Tricholoma matsutake

  • Kim, Jang-Han (Department of Life Science, Graduate School, Dongguk University) ;
  • Han, Yeong-Hwan (Department of Life Science, Dongguk University)
  • Published : 2009.12.31

Abstract

In this study, in an effort to develop a method for the molecular detection of Tricholoma matsutake in Korea from other closely related Tricholomataceae, a species-specific PCR primer pair, TmF and TmR, was designed using nuclear ribosomal intertranscribed spacer (ITS) sequences. The DTmF and DTmR sequences were 5'-CCTGACGCCAATCTTTTCA-3' and 5'- GGAGAGCAGACTTGTGAGCA-3', respectively. The PCR primers reliably amplified only the ITS sequences of T. matsutake, and not those of other species used in this study.

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