Mycobiology

The Korean Society of Mycology (한국균학회)
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Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum

  • Jo, Woo-Sik (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Bae, Soon-Hwa (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Cho, Doo-Hyun (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Park, So-Deuk (Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration) ;
  • Yoo, Young-Bok (Mushroom Research Division Department of Herbal Crop Research National Inst. of Horicultural & Herbal Science, RDA) ;
  • Park, Seung-Chun (College of Veterinary Medicine, Kyeongbuk National University)
  • Published : 2009.12.31
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Abstract

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the use of four dyes, Congo Red, Phenol Red, Remazol Brilliant Blue, and Trypan Blue. To observe the effect of pH on the chromogenic reaction, we also made and tested various media spanning acidic and alkaline pHs, ranging from 4.5 to 8.0. Furthermore, in order to research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to $35{^{\circ}C}$. On the whole, the best protocol called for Ganoderma lucidum transfer onto media containing Congo red with pH adjusted to 7.0, followed by incubation at $25{^{\circ}C}$ for 5 days. Our results will be useful to researchers who aim to study extracellular enzyme activity in Ganoderma lucidum.

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References

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