DOI QR코드

DOI QR Code

Quality Evaluation of Moutan Cortex Radicis Using Multiple Component Analysisby High Performance Liquid Chromatography

  • Ding, Yan (College of Pharmacy, Chungnam National University) ;
  • Wu, Enqi (College of Pharmacy, Chungnam National University) ;
  • Chen, Jianbo (College of Pharmacy, Chungnam National University) ;
  • Nguyen, Huu-Tung (College of Pharmacy, Chungnam National University) ;
  • Do, Thi-Ha (College ofBae Pharmacy, Chungnam National University) ;
  • Park, Kyung-Lae (College ofBae Pharmacy, Chungnam National University) ;
  • Bae, Ki-Hwan (College ofBae Pharmacy, Chungnam National University) ;
  • Kim, Young-Ho (College ofBae Pharmacy, Chungnam National University) ;
  • Kang, Jong-Seong (College ofBae Pharmacy, Chungnam National University)
  • 발행 : 2009.10.20

초록

A simple high performance liquid chromatographic method was developed to evaluate the quality of Moutan Cortex Radicis based on chromatographic fingerprints that characterize eight pharmacological compounds, namely, gallic acid, paeoniflorin, galloyl paeoniflorin, benzoic acid, quercetin, benzoylpaeoniflorin, paeoniflorigenone, and paeonol. These compounds were identified by their characteristic UV profiles and the mass spectroscopy data, and their contents were determined by HPLC. The chromatographic separation was performed on a $C_{18}$ column by gradient elution with 0.05% formic acid in water and acetonitrile. The methodological validation gave acceptable linearities (r = 0.9996) and recoveries (ranging from 99.4∼103.1%). The limits of detection (LOD) of these compounds ranged from 10 to 30 $\mu$g/mL. The representative chromatographic fingerprints of Moutan Cortex Radicis were obtained by analyzing 20 batches of samples collected from markets in Korea and China. For the efficient evaluation of quality for the commercial Moutan Cortex Radicis it is recommended that the total content of the six characteristic compounds should contain more than a minimum of 2% and that the content of total paeoniflorin and paeonol should exceed a minimum of 1.5% of dry weight of Moutan Cortex Radicis.

키워드

참고문헌

  1. Tyler, V. E. J. Nat. Prod. 1999, 62, 1589. https://doi.org/10.1021/np9904049
  2. Shinichi, T.; Tamaki, M.; Tetsuya, A.; Li, X.; Toshiaki, M.; Seiji, I. Neurosci. Lett. 2004, 370, 130. https://doi.org/10.1016/j.neulet.2004.08.043
  3. Kim, H. K.; Tak, J. H.; Ahn, Y. J. J. Agr. Food Chem. 2004, 52, 7857. https://doi.org/10.1021/jf048708a
  4. Wong, Y. F.; Zhou, H.; Wang, J. R.; Xie, Y.; Xu, H. X.; Liu, L. Phytomed. 2008, 15, 416. https://doi.org/10.1016/j.phymed.2008.02.008
  5. Ko, C. H.; Li, K.; Ng, P. C.; Fung, K. P.; Wong, R. P. O.; Chui, K. M.; Gu, G. J. S.; Yung, E.; Fok, T. F. Toxicol. in Vitro 2008, 22, 1222. https://doi.org/10.1016/j.tiv.2008.03.017
  6. Kim, J. J.; Lee, H. Y.; Lee, Y. S.; Oh, B. G.; Cho, C. W.; Kim, Y. S.; Shin, M. K.; Hong, M. C.; Jung, S. K.; Bae, H. S. J. Ethnopharmacol. 2007, 114, 186. https://doi.org/10.1016/j.jep.2007.07.038
  7. Chung, H. S.; Kang, M. K.; Cho, C. W.; Parvez, S.; Park, C. H.; Kim, D. W.; Oh, J. H.; Kim, H. Y.; Shin, M. K.; Hong, M. C.; Kim, Y. S.; Bae, H. S. Biol. Pharm. Bull. 2007, 30, 912. https://doi.org/10.1248/bpb.30.912
  8. Yoo, M. Y.; Lee, B. H.; Choi, Y. H.; Lee, J. W.; Seo, J. H.; Oh, K. S.; Koo, H.-N.; Seo, H. W.; Yon, G. H.; Kwon, D. Y.; Kim, Y. S.; Ryu, S. Y. Planta Med. 2006, 72, 1338. https://doi.org/10.1055/s-2006-951678
  9. KFDA, Regulation on Review of Specifications and Test Methods of Drugs; Korea, 2007
  10. Zhong, S. Z.; Ge, Q. H.; Qu, R.; Li, Q.; Ma, S. P. J. Neurol. Sci. 2009, 277, 58. https://doi.org/10.1016/j.jns.2008.10.008
  11. Kim, S. H.; Kim, S. A.; Park, M. K.; Kim, S. H.; Park, Y. D.; Na, H. J.; Kim, H. M.; Shin, M. K.; Ahn, K. S. Int. J. Immunopharmacol. 2004, 4, 279. https://doi.org/10.1016/j.intimp.2003.12.013
  12. Tsai, H. Y.; Lin, H. Y.; Fong, Y. C.; Wu, J. B.; Chen, Y. F.; Tsuzuki, M.; Tang, C. H. Eur. J. Pharmacol. 2008, 588, 124. https://doi.org/10.1016/j.ejphar.2008.04.024
  13. Sun, G. P.; Wan, X.; Xu, S. P.; Wang, H.; Liu, S. H.; Wang, Z. G. Dis. Esophagus. 2008, 21, 723. https://doi.org/10.1111/j.1442-2050.2008.00840.x
  14. Chae, H. S.; Kang, O. H.; Lee, Y. S.; Choi, J. G.; Oh, Y. C.; Jang, H. J.; Kim, M. S.; Kim, J. H.; Jeong, S. I.; Kwon, D. Y. Am. J. Chin. Med. 2009, 37, 181. https://doi.org/10.1142/S0192415X0900676X
  15. Yang, H. O.; Ko, W. K.; Kim, J. Y.; Ro, H. S. Fitoterapia 2004, 75, 45. https://doi.org/10.1016/j.fitote.2003.08.016
  16. Yang, H. O.; Ko, W. K.; Kim, J. Y.; Ro, H. S. Fitoterapia 2004, 75, 45. https://doi.org/10.1016/j.fitote.2003.08.016
  17. Yoshikawa, M.; Uchida, E.; Kawaguchi, A.; Kitagawa, I.; Yamahara, J. Chem. Pharm. Bull. 1992, 40, 2248. https://doi.org/10.1248/cpb.40.2248
  18. Yoshikawa, M.; Ohta, T.; Kawaguchi, A.; Matsuda, H. Chem. Pharm. Bull. 2000, 48, 1327. https://doi.org/10.1248/cpb.48.1327
  19. Ishida, H.; Takamatsu, M.; Tsuji, K.; Kosuge, T. Chem. Pharm. Bull. 1987, 35, 849. https://doi.org/10.1248/cpb.35.849
  20. 20Pham, H. Y.; Phan, V. K.; Nguyen, X. N.; Nguyen, H. T.; Tran H. Q.; Chau, V. M.; Kim, J. W.; Choi, E. M.; Kim, Y. H. Arch. Pharm. Res. 2007, 30, 1179. https://doi.org/10.1007/BF02980258
  21. Kwon, O. G.; Kim, S. H.; Chun, B. Y.; Park, C. K.; Son, K. H. Kor. J. Pharmacogn. 1999, 30, 340.
  22. Kwon, O. G.; Kim, S. H.; Chun, B. Y.; Park, C. K.; Son, K. H. Kor. J. Pharmacogn. 1999, 30, 340.
  23. Zhang, H. J.; Shen, P.; Cheng, Y. Y. J. Pharmaceut. Biomed. 2004, 34, 705. https://doi.org/10.1016/S0731-7085(03)00650-2
  24. Kim, N. S.; Park, K. R.; Park, I. S.; Park, Y. H. Food Chem. 2006, 96, 496. https://doi.org/10.1016/j.foodchem.2005.04.011
  25. Song, Y.; He, Q.; Li, P.; Cheng, Y. Y. J. Sep. Sci. 2008, 31, 64. https://doi.org/10.1002/jssc.200700259
  26. He, Q.; Ge, Z. W.; Song, Y.; Cheng, Y. Y. Chem. Pharm. Bull. 2006, 54, 1271. https://doi.org/10.1248/cpb.54.1271
  27. Zhao, X. F.; Sun, Y. Q. Anal. Sci. 2003, 19, 1313. https://doi.org/10.2116/analsci.19.1313
  28. Wu, Y. T.; Huang, W. Y.; Lin, T. C.; Sheu, S. J. J. Sep. Sci. 2003, 26, 1629. https://doi.org/10.1002/jssc.200301587
  29. Yu, K.; Wang, Y. W.; Cheng, Y. Y. J. Pharmaceut. Biomed. 2006, 40, 1257 https://doi.org/10.1016/j.jpba.2005.09.005

피인용 문헌

  1. SIMULTANEOUS DETERMINATION OF PAEONOL AND FERULIC ACID IN SU-XIAO-XIN-TONG DROPPING PILLS BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH MATRIX SOLID-PHASE DISPERSION EXTRACTION vol.34, pp.17, 2011, https://doi.org/10.1080/10826076.2011.576299
  2. Metabolomic analysis provides novel chemotaxonomic characteristics for phenotypic cultivars of tree peony vol.6, pp.19, 2014, https://doi.org/10.1039/C4AY01028K
  3. Origins, Phytochemistry, Pharmacology, Analytical Methods and Safety of Cortex Moutan (Paeonia suffruticosa Andrew): A Systematic Review vol.22, pp.6, 2017, https://doi.org/10.3390/molecules22060946
  4. Application of Metabolomics to Quality Control of Natural Product Derived Medicines vol.25, pp.6, 2017, https://doi.org/10.4062/biomolther.2016.249
  5. Revealing Metabolomic Variations in Cortex Moutan from Different Root Parts using HPLC–MS method vol.26, pp.1, 2009, https://doi.org/10.1002/pca.2539
  6. Liquid Chromatography-diode Array Detector-electrospray Mass Spectrometry and Principal Components Analyses of Raw and Processed Moutan Cortex vol.12, pp.45, 2009, https://doi.org/10.4103/0973-1296.176046
  7. 모란 추출액에서 paeoniflorin과 paeonol 동시 정량 분석 및 화장품 원료의 품질관리 기준 설정 vol.35, pp.1, 2018, https://doi.org/10.12925/jkocs.2018.35.1.235