Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Electrochemistry of Hemoglobin in the Chitosan and TiO2 Nanoparticles Composite Film Modified Carbon Ionic Liquid Electrode and Its Electrocatalysis

  • Sun, Wei (Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Li, Xiaoqing (Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Liu, Shufeng (Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology) ;
  • Jiao, Kui (Key Laboratory of Eco-Chemical Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology)
  • Published : 2009.03.20
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Abstract

Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and $TiO_2$ nanoparticles (nano-$TiO_2$) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate ($BMIMPF_6$) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (νs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant ($k_s$) were got as 0.422, 0.93 and 0.117 $s^{-1}$, respectively. The fabricated CTS/nano-$TiO_2$/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide ($H_2O_2$), which exhibited a potential application in fabricating a new kind of third generation biosensor.

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