Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Determination of Heparin Using Norfloxacin-cerium Complex as a Fluorescence Probe by Spectrofluorimetry

  • Patil, Shailaja R. (Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University) ;
  • Mote, Umesh S. (Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University) ;
  • Patil, Shivajirao R. (Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University) ;
  • Kolekar, Govind B. (Fluorescence Spectroscopy Research Laboratory, Department of Chemistry, Shivaji University)
  • Published : 2009.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

A simple, rapid, practical and sensitive spectofluorimetric method was developed for the determination of trace amount of heparin (Hep). Under the Optimum conditions, we studied the interaction between NFLX-Ce$^{3+}$-Hep complex by using absorption and fluorescence spectra. It was observed that Hep remarkably enhance the fluorescence intensity of the NFLX-Ce$^{3+}$ complex at ${\lambda}$= 356 nm in the buffer solution of pH = 7.60 and the enhancement effect is shown to relate with the concentration of Hep. The linear range and detection limit for the determination of Hep was obtained. By the Rosenthal graphic method, the association constant (K) and binding numbers (N) of Hep with probe were investigated. This method is relatively free of interference from coexisting substances and successfully applied for the determination of heparin in heparin sodium injection samples. A suitable mechanism of fluorescence enhancement between NFLX-Ce$^{3+}$ and the NFLX-Ce$^{3+}$-Hep systems were proposed and discussed.

Keywords

References

  1. Mathison, S.; Bakker, E. Anal. Chem. 1999, 71, 4614 https://doi.org/10.1021/ac990387s
  2. Wang, R. Handbook of Chemical Products; Medicament (part3): Peking China, 1999; p. 704
  3. Jiao, Q. C.; Liu, Q.; Sun, C.; He, H. Talanta 1999, 48, 1095 https://doi.org/10.1016/S0039-9140(98)00330-0
  4. Nemcova, I.; Rychlovsky, P.; Havelcova, M.; Brabcova, M. Anal. Chim. Acta 1999, 401, 223 https://doi.org/10.1016/S0003-2670(99)00472-9
  5. Zhou, X.; Liu, J.; Zhang, M.; Chen, S. Talanta 1998, 46, 757 https://doi.org/10.1016/S0039-9140(98)00040-X
  6. Gaus, K.; Hall, E. A. H. Biosen. Bioelectr. 1998, 13, 1307 https://doi.org/10.1016/S0956-5663(98)00094-3
  7. Ramamurthy, N.; Baliga, N.; Wahr, J. A.; Schaller, U.; Yang, V. C.; Meyerhoff, M. E. Clin. Chem. 1998, 44, 606
  8. Ambrose, T. M.; Parvin, C. A.; Mendeloff, E. L.; Luchtman-Jones, L. Clin. Chem. 2001, 47, 858
  9. Wei, W.; Wang, H.; Jiang, C. Spctrochim. Acta A 2006, 63, 241 https://doi.org/10.1016/j.saa.2005.03.001
  10. Wang, H.; Wang, Y.; Jiang, C. Anal. Letters 2005, 38, 167 https://doi.org/10.1081/AL-200043475
  11. Zhu, X.; Wang, X.; Jiang, C. Anal. Biochem. 2005, 341, 299 https://doi.org/10.1016/j.ab.2005.03.014
  12. Richardson, F. S. Chem. Rev. 1982, 82, 541 https://doi.org/10.1021/cr00051a004
  13. Rosenthal, H. E. Anal. Biochem. 1967, 20, 525 https://doi.org/10.1016/0003-2697(67)90297-7
  14. Guo, Z. X.; Shen, H. X. Analyst 1999, 124, 1093 https://doi.org/10.1039/a902253h
  15. Hao, Y. M.; Shen, H. X. Spectrochim. Acta part A 2000, 56, 1013 https://doi.org/10.1016/S1386-1425(99)00286-3

Cited by

  1. Determination of heparin using terbium-danofloxacin as a luminescent probe vol.80, pp.1, 2013, https://doi.org/10.1007/s10812-013-9728-1
  2. Spectrometric methods in pharmaceutical analysis of glycosaminoglycans: the state-of-the-art vol.151, pp.8, 2020, https://doi.org/10.1007/s00706-020-02632-3