Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Capillary Gel Electrophoretic Analysis of Cattle Breeds Based on Difference of DNA Mobility of Microsatellite Markers

  • Lee, Mi-Ji (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University) ;
  • Yoon, Du-Hak (National Institute of Animal Science) ;
  • Jeon, Jin-Tae (Division of Applied Life Science (BK21 program), Graduate School of Gyeongsang National University) ;
  • Eo, Seong-Kug (Laboratory of Microbiology, College of Veterinary Medicine, Chonbuk National University) ;
  • Kang, Seong-Ho (Department of Chemistry and Research Institute of Physics and Chemistry (RINPAC), Chonbuk National University)
  • Published : 2009.11.20
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Abstract

A breed of cattle, i.e., Korean cattle (Hanwoo), was identified based on the DNA mobilities of their microsatellites (MSs) by capillary gel electrophoresis (CGE) with a laser-induced fluorescence (LIF) detector. The MS markers were used for the accurate identification of species-specific genes. The DNA mobilities of the MS markers of Hanwoo and Holstein were measured using a CGE system with a fused-silica capillary (inner diameter of 75 ${\mu}m$, outer diameter of 365 ${\mu}m$, and total length of 50 cm). The capillary was dynamically coated with 1.0% (w/v) polyvinylpyrrolidone ($M_r$ = 1,000,000) and then filled with a mixture of 1.3% (w/v) poly(ethylene oxide) ($M_r$ = 600,000) and 1.9% (w/v) poly(ethylene oxide) (Mr = 8,000,000) as a sieving gel matrix. The species-specific genes of Hanwoo and Holstein were clearly distinguished within 33 min. This CGE assay technique is expected to be a useful analytical method for the fast and accurate identification of breeds of cattle.

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