Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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An ELISA-on-a-Chip Biosensor System for Early Screening of Listeria monocytogenes in Contaminated Food Products

  • Seo, Sung-Min (Program for Bio-Microsystem Technology, Korea University) ;
  • Cho, Il-Hoon (Program for Bio-Microsystem Technology, Korea University) ;
  • Kim, Joo-Ho (Graduate School of Life Sciences and Biotechnology,Korea University) ;
  • Jeon, Jin-Woo (Program for Bio-Microsystem Technology, Korea University) ;
  • Oh, Eun-Gyoung (Food Safety Research Center, National Fisheries Research and Development Institute) ;
  • Yu, Hong-Sik (Food Safety Research Center, National Fisheries Research and Development Institute) ;
  • Shin, Soon-Bum (Food Safety Research Center, National Fisheries Research and Development Institute) ;
  • Lee, Hee-Jung (Food Safety Research Center, National Fisheries Research and Development Institute) ;
  • Paek, Se-Hwan (Department of Biotechnology, Korea University)
  • Published : 2009.12.20
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Abstract

An enzyme-linked immunosorbent assay (ELISA)-on-a-chip (EOC) biosensor combined with cell concentration technology based on immuno-magnetic separation (IMS) was investigated for use as a potential tool for early screening of Listeria monocytogenes (L. monocytogenes) in food products. The target analyte is a well-known pathogenic foodborne microorganism and outbreaks of the food poisoning typically occur due to contamination of normal food products. Thus, the aim of this study was to develop a rapid and reliable sensor that could be utilized on a daily basis to test food products for the presence of this pathogenic microorganism. The sensor was optimized to provide a high detection capability (e.g., 5.9 ${\times}\;10^3$ cells/mL) and, to eventually minimize cultivation time. The cell density was condensed using IMS prior to analysis. Since the concentration rate of IMS was greater than 100-fold, this combination resulted in a detection limit of 54 cells/mL. The EOC-IMS coupled analytical system was then applied to a real sample test of fish intestines. The system was able to detect L. monocytogenes at a concentration of 2.4 CFU/g after pre-enrichment for 6 h from the onset of cell cultivation. This may allow us to monitor the target analyte at a concentration less than 1 CFU/g within a 9 h-cultivation provided a doubling time of 40 min is typically maintained. Based on this estimation, the EOC-IMS system can screen and detect the presence of this microorganism in food products almost within working hours.

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References

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