Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Interaction of ct-DNA with 2,4-Dihydroxy Salophen

  • Azani, Mohammad-Reza (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan) ;
  • Hassanpour, Azin (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan) ;
  • Bordbar, Abdol-Khalegh (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan) ;
  • Mirkhani, Valiollah (Laboratory of Biophysical Chemistry, Department of Chemistry, University of Isfahan)
  • Published : 2009.09.20
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Abstract

In the present study, at first, 2,4-Dihydroxy Salophen (2,4-DHS), has been synthesized by combination of 1, 2-diaminobenzene and 2,4-dihydroxybenzaldehyde in a solvent system. This ligand containing meta-quinone functional groups were characterized using UV-Vis and IR spectroscopies. Subsequently, the interaction between native calf thymus deoxyribonucleic acid (ct-DNA) and 2,4-DHS, was investigated in 10 mM Tris/HCl buffer solution, pH 7.2, using UV-visible absorption and fluorescence spectroscopies, thermal denaturation technique and viscosity measurements. From spectrophotometric titration experiments, the binding constant of 2,4-DHS with ct-DNA was found to be (1.1 ${\pm}\;0.2)\;{\times}\;10^4\;M^{-1}.$ The fluorescence study represents the quenching effect of 2,4-DHS on bound ethidium bromide to DNA. The quenching process obeys linear Stern-Volmer equation in extended range of 2,4-DHS concentration. Thermal denaturation experiments represent the increasing of melting temperature of DNA (about 3.5 ${^{\circ}C}$) due to binding of 2,4-DHS. These results are consistent with a binding mode dominated by interactions with the groove of ct-DNA.

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References

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