Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Identification of B52-dependent Gene Expression Signature and Alternative Splicing Using a D. melanogaster B52-null Mutant

  • Hong, Sun-Woo (Global Research Laboratory for RNAi Medicine, Department of Chemistry and BK21 School of Chemical Materials Science, Sungkyunkwan University) ;
  • Jung, Mi-Sun (Department of Biomedical Engineering, Dongguk University) ;
  • Kim, Eun-Kyung (Department of Biomedical Engineering, Dongguk University) ;
  • Lee, Dong-Ki (Global Research Laboratory for RNAi Medicine, Department of Chemistry and BK21 School of Chemical Materials Science, Sungkyunkwan University) ;
  • Kim, So-Youn (Department of Biomedical Engineering, Dongguk University)
  • Published : 2009.02.20
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Abstract

SR proteins are essential splicing regulators and also modulate alternative splicing events, which function both as redundant and substrate-specific manner. The Drosophila B52/SRp55, a member of the SR protein family, is essential for the fly development in vivo, as deletion of B52 gene results in lethality of animals at the second instar larval stage. Identification of the splicing target genes of B52 thus should be crucial for the understanding of the specific developmental role of B52 in vivo. In this study, we performed whole-genome DNA microarray experiments with a B52- knock-out animal. Analysis of the microarray data not only provided the B52-dependent gene expression signature, but also revealed a larval-stage specific, alternative splicing target gene of B52. Our result thus provides a starting point to understand the essential function of B52 at the organismal level.

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References

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