Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
권호 표지

Herbicide-resistant Transgenic Mongolian Bentgrass (Agrostis mongolica Roshev.) obtained by Agrobacterium-mediated Transformation

  • Vanjildorj, Enkhchimeg (College of Applied Life Sciences, Cheju National University) ;
  • Bae, Tae-Woong (Subtropical Horticulture Research Center, Cheju National University) ;
  • Song, In-Ja (Subtropical Horticulture Research Center, Cheju National University) ;
  • Kim, Kyung-Moon (Subtropical Horticulture Research Center, Cheju National University) ;
  • Lim, Yong-Pyo (Department of Horticulture, Chungnam National University) ;
  • Lee, Hyo-Yeon (College of Applied Life Sciences, Cheju National University)
  • Received : 2008.06.16
  • Published : 2008.06.10
⟨ Previous Next ⟩

Abstract

Herbicide resistance is the most common trait being tested and thus herbicide?resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide?resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium?mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S?bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.

Keywords

References

  1. Asano Y, Ito Y, Fukami M, Sugiura K, Fujiie A. 1998. Herbicide‐resistant transgenic creeping bentgrass plants obtained by electroporation using an altered buffer. Plant Cell Rep. 17:963‐967
  2. Belanger FC, Meagher TR, Day PR, Plumley K, Meyer WA. 2003. Interspecific hybridization between Agrostis stolonifera and related Agrostis species under field conditions. Crop Sci. 43:240-246 https://doi.org/10.2135/cropsci2003.0240
  3. Chai ML, Senthil KK, Kim DH. 2004. Transgenic plants of colonial bentgrass from embryogenic callus via Agrobacterium-mediated transformation. Plant Cell Tiss. Org. Cult. 77:165-171 https://doi.org/10.1023/B:TICU.0000016823.94547.c7
  4. Chai ML, Wang BL, Kim JY, Lee JM, Kim DH. 2003. Agrobacterium-mediated transformation of herbicide resistance in creeping bentgrass and colonial bentgrass. J. Zhejiang Univ. Sci. 4:346-351 https://doi.org/10.1631/jzus.2003.0346
  5. Enkhchimeg V, Bae TW, Riu KZ, Kim SY, Lee HY. 2005. Overexpression of Arabidopsis ABF3 gene enhances tolerance to drought and cold in transgenic lettuce (Lactuca sativa L.). Plant Cell Tiss. Org. Cult. 83:41-50 https://doi.org/10.1007/s11240-005-3800-3
  6. Fu D, Tisserat NA, Xiao Y, Settle D, Muthukrishnan S, Liang GH. 2005. Overexpression of rice TLPD34 enhances dollar-spot resistance in transgenic bentgrass. Plant Sci. 168:671-680 https://doi.org/10.1016/j.plantsci.2004.09.032
  7. Han N, Chen D, Bian HW, Deng MJ, Zhu MY. 2005. Production of transgenic creeping bentgrass Agrostis stolonifera var. palustris plants by Agrobacterium tumefaciens-mediated transformation using hygromycin selection. Plant Cell, Tissue, Organ Cult. 81:131-138 https://doi.org/10.1007/s11240-004-4042-5
  8. Kwon YW, Kim DS. 2001. Herbicide-resistant genetically-modified crop: its risks with an emphasis on gene flow. Weed Biol. Manag. 1: 42-52 https://doi.org/10.1046/j.1445-6664.2001.00001.x
  9. James C. 2006. Executive Summary of global status of commercialized biotech/GM crops: 2006. ISAAA Briefs No. 35. ISAAA: Ithaca, NY
  10. Luo H, Hu Q, Nelson K, Longo C, Kausch AP, Chandlee JM, Wipff JK, Fricker CR. 2004. Agrobacterium tumefaciens-mediated creeping bentgrass (Agrostis stolonifera L.) transformation using phosphinothricin selection results in a high frequency of single-copy transgene integration. Plant Cell Rep. 22:645-652 https://doi.org/10.1007/s00299-003-0734-2
  11. Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Plant Physiol. 15:473-479 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  12. Saghai-Maroof MA, Soliman K, Jorgensen RA, Allard RW. 1984. Ribosomal DNA spacer-length polymorphism in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc. Nat'l. Acad. Sci. USA. 81:8014-8018 https://doi.org/10.1073/pnas.81.24.8014
  13. Toyama K, Bae CH, Kang JG, Lim YP, Adachi T, Riu KZ, Song PS, Lee HY. 2003. Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation. Mol Cells. 16:19-27
  14. Vergara GV, Bughrara SS. 2003. AFLP analyses of genetic diversity in bentgrass. Crop Sci. 43:2162-2171 https://doi.org/10.2135/cropsci2003.2162
  15. Wang ZY, Ge Y. 2005. Rapid and efficient production of transgenic bermudagrass and creeping bentgrass by passing the callus formation phase. Func. Plant Biol. 32: 769-776 https://doi.org/10.1071/FP05083
  16. Yu TT, Skinner DZ, Liang GH, Trick HN, Huang B, Muthukrishnan S. 2000. Agrobacterium-mediated transformation of creeping bentgrass using GFP as a reporter gene. Hereditas. 133:229-233 https://doi.org/10.1111/j.1601-5223.2000.00229.x