Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Screening of a Natural Feed Additive Having Anti-viral Activity against Influenza A/H5N1

안전한 닭고기 생산을 위한 고병원성 조류인플루엔자 A/H5N1에 항바이러스 효과를 가진 천연 사료첨가제의 탐색

  • Lee, Jang-Hyun (Division of Animal Life Science, Konkuk University) ;
  • Kwon, Su-Min (Division of Animal Life Science, Konkuk University) ;
  • Seo, Sang-Heui (College of Veterinary Medicin,e Chungnam National University) ;
  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Kim, Young-Bong (Division of Animal Life Science, Konkuk University) ;
  • Kim, Soo-Ki (Division of Animal Life Science, Konkuk University) ;
  • Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
  • 이장현 (건국대학교 동물생명과학부) ;
  • 권수민 (건국대학교 동물생명과학부) ;
  • 서상희 (충남대학교 수의과대학) ;
  • 박영서 (경원대학교 식품생물공학과) ;
  • 김영봉 (건국대학교 동물생명과학부) ;
  • 김수기 (건국대학교 동물생명과학부) ;
  • 백현동 (건국대학교 동물생명과학부)
  • Published : 2008.10.31
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Abstract

To search for anit-H5N1 influenza virus agent, the anti-viral activity of methanol and aqueous extracts from thirty medicinal plants were examined in this study. The plant material (30 g) was extracted with methanol (300 mL) for 24 hr at room temperature. Methanol extracts were filtered and evaporated, then freeze-dried. Aqueous extracts were prepared with dried plant material (30 g) and hot distilled water (300 mL). After 3 hr, the aqueous extracts were filtered and evaporated, then lyophilized. Extracts prepared from different plants were tested the antiviral activity against influenza viruses [A/vietnam/1194/04 (H5N1)-NIBRG-14] using the hemagglutination (HA) assay. Among the test plants, Asarum sieboldii was found to be a potent inhibitor of H5N1 influenza virus in MDCK cell culture. Virus titers were 7 log, whereas with methanol extract of Asarum sieboldii for 48 hr titers were 3 log, indicating that methanol extract of Asarum sieboldii inhibited the H5N1 influenza viruses from the infected cells.

Keywords

References

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