The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of Codonopsis lanceolata Extracts on Mouse Immune Cell Activation

더덕 추출물이 마우스 면역세포 증식에 미치는 영향

  • Ryu, Hye-Sook (Dept. of Food and Nutrition, College of Health Sciences, Sangji University)
  • 류혜숙 (상지대학교 보건과학대학 식품영양학과)
  • Published : 2008.09.30
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Abstract

Codonopsis lanceolata has long been used as a seasonal food and as a traditional tonic medicine with anti-inflammatory and anti-oxidation properties. The present study investigated the in vitro effect of Codonopsis lanceolata extracts on immune function in mice. After preparing a single cell suspension splenocyte proliferation was determined by the MTT(3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. The cytokines IL-1${\beta}$, IL-6, and TNF-$\alpha$ were not secreted by macrophages stimulated with or without LPS as determined by an ELISA cytokine kit assay. After a 48-hr incubation with the mitogens ConA or LPS there was an increase in splenocytes proliferation and in the production of IL-1${\beta}$, IL-6, and TNF-$\alpha$ in the suspensions supplemented with 50, 100, 250, 500 ${\mu}g/m{\ell}$ Codonopsis lanceolata water extract. The results suggest Codonopsis lanceolata water extract may enhance immune function by regulating splenocyte proliferation and stimulating cytokine production.

Keywords

References

  1. Kim, HP, Son, KH and Kang, SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J. Pharmacological Sci. 96:229-245. 2004 https://doi.org/10.1254/jphs.CRJ04003X
  2. Park, JS and Chyun, JH. Effects of low fat diet and saturated fat supplementation on the immune status of BALB/c mouse. Kor. J. Nutr. 26:578-585. 1993
  3. Kim, GH and Sunwoo, YK. Effects of small water dropwort extract on cellular immune response of mice. J. Bacteriolo. Virol. 28:419-430. 1993
  4. Park, HA. Enhancing effect of Ixeris sonchifolia Hance, Oenanthe javanica, and. Fagopyrum escluentum Moench on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni., Seoul. 2003
  5. Ryu, HS and Kim, HS. Effects of job's tear extracts on mouse immune cell ativation. J. Kor. Diet Assoc. 11:44-50. 2005
  6. Ko, MS, Shin, KM and Lee, MY. Effects of Hizikia fusiforme ethanol extract on antioxidative enzymes in ethanol induced hepatotoxicity of rat liver. J. Kor. Soc. Food Sci. Nutr. 31:87-91. 2002 https://doi.org/10.3746/jkfn.2002.31.1.087
  7. Joung, YH. Effect of Hizikia fusiforme water extracts on mouse immune cell activation. MS. Thesis, Sookmyung Women's Uni., Seoul. 2003
  8. Ryu, HS, Kim, J and Kim, HS. Enhancing effect of Sorghum bicolor L. Moench(Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Kor. J. Food Nutr. 19: 176-182. 2006
  9. Yang, HS, Chio, SS, Han, BH, Kang, SS and Woo, WS. Sterols and tripenoids from Codonopsis lanceolata. J. Pharm. Soc. Kor. 19:43-50. 1795
  10. Han, BH, Kang, SS and Woo, WS. Triterpenoid from Codonopsis lanceolata. J. Pharm. Soc. Kor. 20:145-152. 1976
  11. Park, JY, Kim, SY and Kwag, J. Volatile flavor components of Codonopsis lanceolata. J. Kor. Agric. Chem. Soc. 32: 338-343. 1989
  12. Han, EG, Sung, IS, Moon, HG and Cho, SY. Effect of Codonopsis lanceolata water extract on the level of lipid in rats fed diet. J. Kor. Soc. Food Sci. Nutr. 27:940-944. 1998
  13. Maeng, YS and Park, HK. Antioxidant activity of ethanol extracts from Dodok. Kor. J. Food Sci. Technol. 23:311-316. 1991
  14. Han, EG and Cho, SY. Effects of Codonopsis lanceolata water extract on the activities of antioxidative enzymes in carbon tetrachloride treated rats. J. Kor. Soc. Food Sci. Nutr. 26: 1181-1186. 1997
  15. Glatthaar-Saalumller, B, Sacher, F and Esperester, A. Antiviral activiral activity of an extract drived from roots of Eleutheroccus senticosus. Antivir. Res. 50:223-228. 2001 https://doi.org/10.1016/S0166-3542(01)00143-7
  16. Yoo, BH, Lee, SW, Shin, KS, Choi, WH, Hwang, SH, Seo, SH, Kim, SH and Park, WM. Effect of hot water extract from Acanthopanax senticosus on systemic anaphylaxis. Kor. J. Food Sci. Technol. 34:518-523. 2002
  17. Hirata, F, Fugita, K, Ishikura, Y, Hosodo, K and Ishikawa, H. Hypocholesterolemic effect of sesame ligan in humans. Atherosclerosis. 122:135-136. 1996 https://doi.org/10.1016/0021-9150(95)05769-2
  18. Lee, JH. Immunostimulative effect of hot-water extracts from Codonopsis lanceolata on lymphocyte and clonal macrophage. Kor. J. Food Sci. Technol. 34:732-736. 2002
  19. Suh, JS. Effect of Codonopsis lanceolata Radix water extract on immunocyte. Kor. J. Food Nutr. 9:379-384. 1996
  20. Lim, SD, Seong, KS, Kim, KS and Han, DU. Effects of fermented milk hot water extracts from Acanthopanax senticosus and Codonopsis lanceolata on the immune status of mouse. Kor. J. Food Sci. Technol. 39:323-329. 2007
  21. Mishell, BB and Shiigi, SM. Selected Methods in Cellular Immunology. 1st ed. Sanfrancisco. WH Freeman and Co. 4. 1980
  22. Choi, SE. Effect of needle extracts on mouse splenocyte proliferation and cytokine production by activated mouse peritoneal macrophage. MS. Thesis, Sookmyung Women's Uni., Seoul. 2000
  23. Park, KY, Lee, SJ, Lee, KI and Rhee, SH. The antitumor effect in Sarcoma-180 tumor cell of mice administered with Japanese apricot, garlic ginger doenjang. Kor. J. Food Cookery Sci. 21:599-606. 2005
  24. Ryu, HS, Kim, J and Kim, HS. Enhancing effect of Sorghum bicolor L. Moench(Sorghum, su-su) extracts on mouse spleen and macrophage cell activation. Kor. J. Food Nutr. 19: 176-182. 2006
  25. Kim, HP, Son, KH, Chang, HW and Kang, SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J. Pharmacological Sci. 96:229-245. 2004 https://doi.org/10.1254/jphs.CRJ04003X
  26. Barnes, PJ and Liew, FY. Nitric oxide and asthmatic inflammation. Immunology Today. 16:128-130. 1995 https://doi.org/10.1016/0167-5699(95)80128-6
  27. Lee, YJ, Kim, JM and Jung, YM. Effect of Codonopsis pilosula on the cellular immunity. Kor. J. Publ. Hlth. 19:273-279. 1995
  28. Balkwill, FR, Maylor, MS and Malik, S. Tumor necrosis factor as an anti cancer agent. Eur. J. Cancer. 26:641-644. 1990 https://doi.org/10.1016/0277-5379(90)90097-D
  29. Chao, CC, Hu, S, Molitor, TW, Brosnan, CF and Berman, JW. Cytokine prodution by human fetal microglia and astrocytes. J. Immunol. 150:2659-2660. 1998
  30. Lee, YK. A study of immunomodulating effects and production of nitric oxide by C. cristata L. extracts in mice. MS. Thesis, Sookmyung Women's Uni., Seoul. 2001
  31. Ryu, HS and Kim, HS. Effect of Zingiber officinale Roscoe extracts on mice immune cell activation. Kor. J. Nutr. 37: 23-30. 2004
  32. Suh, JS. Effect of Codonopsis lanceolata Radix water extract on immunocytes. Kor. J. Food Nutr. 9:379-384. 1996
  33. Suh, JS and Eun, JS. Isolation of active component on immunocytes from Codonopsis lanceolata. Kor. J. Nutr. 31:1076-1081. 1996