Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum)

희귀 및 멸종위기 식물 덩굴용담의 기내생장에 미치는 광질 및 환기효과

  • Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute(KFRI)) ;
  • Park, So-Young (Division of Biotechnology, Korea Forest Research Institute(KFRI))
  • 문흥규 (국립산림과학원 생물공학과) ;
  • 박소영 (국립산림과학원 생물공학과)
  • Published : 2008.09.30
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Abstract

Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.

소멸위기 식물인 덩굴용담을 재료로 기내 생장 및 발근에 미치는 광질 및 환기효과를 조사하였다. 무처리 혹은 환기가 가능한 2종의 배양용기에 신초를 배양한 다음 5가지의 서로 다른 광질 1) 형광등, 2) 100% 적색광(R), 3) 70% 적색광+30% 청색광(R7B3), 4) 50% 적색광+50% 청색광(R5B5), 및 5) 100% 청색광 하에서 4주간 배양하였다. 실험을 통해 얻은 주요 결과는 다음과 같다. 1. 발근율은 형광등과 적색광에서 $80{\sim}100%$로 높았고, 특히 환기 처리시 100%의 발근율을 보였다. 광질별로는 적색광이 절간신장을 촉진시키고 청색광은 억제하는 경향이었다. 전반적으로 환기처리 시 진녹색의 큰 잎으로 자랐고, 액아의 발달을 억제하여 1-2개의 우세줄기로 생장하였다. 2. 식물체의 총 생체중은 적색광과 청색광이 7:3의 비율로 혼합된 혼합광 R7B3에서 가장 높았고, 특히 환기처리 시 식물체 당 257.7 mg으로 가장 높았다. 식물체의 충실을 나타내는 건물율(dry matter)은 적색광에서 가장 낮았고, 청색광에서 15%로 가장 높았으며, 청색광의 비율이 높아짐에 따라 건물율이 높아지는 경향을 보였다. 3. 광질별 총 엽록소 함량은 환기처리 하의 혼합광 R7:B3 및 R5:B5에서 29.5 ${\mu}g/g$ FW와 31.2 ${\mu}g/g$ FW으로 각각 가장 높게 나타났다. 광합성에 관여하는 carotenoid 함량에 있어서도 모든 광질에서 환기 처리 시 높게 나타났다. 이상의 결과는 덩굴용담의 기내배양은 배양목적에 따른 광질의 선택이 중요한 요소이며, 발근을 위해서는 적색광을 처리함이 좋고, 건전한 식물체의 생장은 혼합광 R7:B3에서 양호한 것으로 나타났다. 특히 모든 광질 하에서 환기처리가 건전한 줄기생장 및 발근을 촉진하는 것으로 나타나 이 식물의 효율적인 기내생장을 위해서 반드시 고려해야 할 내용으로 관찰되었다.

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