Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Active Component of Fatsia japonica Enhances the Transduction Efficiency of Tat-SOD Fusion Protein both In Vitro and In Vivo

  • Lee, Sun-Hwa (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kim, So-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Kim, Dae-Won (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Jang, Sang-Ho (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Lim, Soon-Sung (Silver Biotechnology Research Center, Hallym University) ;
  • Kwon, Hyung-Joo (Department of Microbiology, College of Medicine, Hallym University) ;
  • Kang, Tae-Cheon (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Won, Moo-Ho (Department of Anatomy and Neurobiology, College of Medicine, Hallym University) ;
  • Kang, Il-Jun (Department of Food and Nutrition, Hallym University) ;
  • Lee, Kil-Soo (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Park, Jin-Seu (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Eum, Won-Sik (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo-Young (Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University)
  • Published : 2008.09.30
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Abstract

It has been reported that Tat-SOD can be directly transduced into mammalian cells and skin and acts as a potential therapeutic protein in various diseases. To isolate the compound that can enhance the transduction efficiency of Tat-SOD, we screened a number of natural products. 3-O-[$\beta$-D-Glucopyranosyl(1$\rightarrow$4)-$\alpha$-L-arabinopyranosyll-hederagenin (OGAH) was identified as an active component of Fatsia japonica and is known as triterpenoid glycosides (hederagenin saponins). OGAH enhanced the transduction efficiencies of Tat-SOD into HeLa cells and mice skin. The enzymatic activities in the presence of OGAH were markedly increased in vitro and in vivo when compared with the controls. Although the mechanism is not fully understood, we suggest that OGAH, the active component of Fatsia japonica, might change the conformation of the membrane structure and it may be useful as an ingredient in anti-aging cosmetics or as a stimulator of therapeutic proteins that can be used in various disorders related to reactive oxygen species (ROS).

Keywords

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