Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Optimization of Extraction of Fpase from the Fermented Bran of Aspergillus niger in Solid State Fermentation

  • Chandra, M. Subhosh (Department of Microbiology, Sri Krishnadevaraya University) ;
  • Reddy, B. Rajasekhar (Department of Microbiology, Sri Krishnadevaraya University) ;
  • Choi, Yong-Lark (Department of Biotechnology, Microbial Molecular Genetics lab, College of Natural Resources and Life Science, Dong-a University)
  • Published : 2008.08.30
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Abstract

A local isolate of Aspergillus niger was cultivated under optimal growth conditions on wheat bran in solid state fermentation. Filter paperase from fermented bran was separately extracted with different solvents to test the recovery of the enzyme. Among solvents tested, distilled water served as the best leachate, thus the conditions were further optimized with distilled water. After two washes of fermented bran with distilled water for 1.5 h each under stationary conditions at 1 g wheat bran: 5 mL distilled water, the maximum recovery of 13.5 $Ug^{-1}$ of wheat bran was obtained.

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