Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Enzymatic Properties of Atrazine Chlorohydrolase Entrapped in Biomimetic Silica

  • Ho, Cuong Tu (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Kang, Su-Il (International Environmental Research Center, Gwangju Institute of Science and Technology) ;
  • Hur, Hor-Gil (Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology)
  • Published : 2008.08.30
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Abstract

Purified atrazine chlorohydrolase (AtzA) was entrapped in the nanoparticles of biomimetically synthesized silica at the ambient condition within 20 min. Entrapped AtzA in biomimetic silica was less affected by pH change and showed higher thermostability than free enzymes. The entrapped AtzA was also more tolerant against proteolysis, with 80% of the initial activity remaining and retained 82% of the initial activity even after four cycles of usage. These results suggest that entrapment of AtzA in biomimetic silica could be utilized under diverse environmental conditions with the active catalytic performance sustained.

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References

  1. Bremner JM and Mulvaney RL (1978) Urease activity in soils. In Soil Enzymes, p. 149, Academic Press Inc., London, UK
  2. Brott LL, Naik RR, Pikas DJ, Kirkpatrick SM, Tomlin DW, Whitlock PW, Clarson SJ, and Stone MO (2001) Ultrafast holographic nanopatterning of biocatalytically formed silica. Nature 413, 291-293 https://doi.org/10.1038/35095031
  3. De Souza ML, Sadowsky MJ, and Wackett LP (1996) Atrazine chlorohydrolase from Pseudomonas sp. strain ADP: gene sequence, enzyme purification, and protein characterization. J Bacteriol 178, 4894-4900 https://doi.org/10.1128/jb.178.16.4894-4900.1996
  4. De Souza ML, Wackett LP, Boundy-Mills KL, Mandelbaum RT, and Sadowsky MJ (1995) Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine. Appl Environ Microbiol 61, 3373-3378
  5. Graziano N, McGuire MJ, Roberson A, Adams C, Jiang H, and Blute N (2006) National atrazine occurrence monitoring program using the Abraxis ELISA method. Environ Sci Technol 40, 1163-1171 https://doi.org/10.1021/es051586y
  6. Jiang H, Adams C, Graziano N, Roberson A, McGuire M, and Khiari D (2006) Occurrence and removal of chloro-s-triazines in water treatment plants. Environ Sci Technol 40, 3609-3616 https://doi.org/10.1021/es052038n
  7. Kauffmann CG and Mandelbaum RT (1996) Entrapment of atrazine-degrading enzymes in sol-gel glass. J Biotechnol 51, 219-225 https://doi.org/10.1016/S0168-1656(96)01599-4
  8. Kauffmann C and Mandelbaum RT (1998) Entrapment of atrazine chlorohydrolase in sol-gel glass matrix. J Biotechnol 62, 169-176 https://doi.org/10.1016/S0168-1656(98)00060-1
  9. Kauffmann C, Shoseyov O, Shpigel E, Bayer EA, Lamed R, Shoham Y, and Mandelbaum RT (2000) Novel methodology for enzymatic removal of atrazine from water by CBD-fusion protein immobilized on cellulose. Environ Sci Technol 34, 1292-1296 https://doi.org/10.1021/es990754h
  10. Knecht MR and Wright DW (2003) Functional analysis of the biomimetic silica precipitating activity of the R5 peptide from Cylindrotheca fusiformis. Chem Commun 3038-3039
  11. Kroger N, Deutzmann R, and Sumper M (1999) Polycationic peptides from diatom biosilica that direct silica nanosphere formation. Science 286, 1129-1132 https://doi.org/10.1126/science.286.5442.1129
  12. Ladd JN, and Butler JH (1972) Short-term assays of soil proteolytic enzyme activities using proteins and dipeptide derivatives as substrates. Soil Biol Biochem 4, 19-30 https://doi.org/10.1016/0038-0717(72)90038-7
  13. Luckarift HR, Spain JC, Naik RR, and Stone MO (2004) Enzyme immobilization in a biomimetic silica support. Nat Biotechnol 22, 211-213 https://doi.org/10.1038/nbt931
  14. Mandelbaum RT, Allan DL, and Wackett LP (1995) Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine. Appl Environ Microbiol 61, 1451-1457
  15. Naik RR, Tomczak MM, Luckarift HR, Spain JC, and Stone MO (2004) Entrapment of enzymes and nanoparticles using biomimetically synthesized silica. Chem Commun 1684-1685
  16. Naik RR, Whitlock PW, Rodriguez F, Brott LL, Glawe DD, Clarson SJ, and Stone MO (2003) Controlled formation of biosilica structures in vitro. Chem Commun 238-239
  17. Shipitalo MJ and Owens LB (2003) Atrazine, deethylatrazine, and deisopropylatrazine in surface runoff from conservation tilled watersheds. Environ Sci Technol 37, 944-950 https://doi.org/10.1021/es020870b
  18. Shtelzer S, Rappoport S, Avnir D, Ottolenghi M, and Braun S (1992) Properties of trypsin and of acid phosphatase immobilized in sol-gel glass matrices. Biotechnol Appl Biochem 15, 227-235
  19. USDA Agricultural chemical usage: 2005 field crops summary (2006) http://usda.mannlib.cornell.edu/usda/nass/AgriChemUsFC//2000s/2006/AgriChemUsFC-05-17-2006.pdf
  20. Wang Y and Caruso F (2004) Enzyme encapsulation in nanoporous silica spheres. Chem Commun 1528-1529