대한기계학회논문집A (Transactions of the Korean Society of Mechanical Engineers A)

  • 제32권12호
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  • Pages.1115-1122
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  • 2008
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  • 1226-4873(pISSN)
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  • 2288-5226(eISSN)
대한기계학회 (The Korean Society of Mechanical Engineers)
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주화성에 의한 활동성 정자 분리용 마이크로 바이오칩에 관한 연구

A Study About Microbiochip for Separation of Motile Sperm by Using Chemotaxis

  • 고용준 (한양대학교 대학원 기계공학과) ;
  • 맹준호 (한양대학교 대학원 생화학과) ;
  • 안유민 (한양대학교 기계공학과) ;
  • 황승용 (한양대학교 분자생명과학부, ㈜지노첵)
  • 발행 : 2008.12.01
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초록

This paper presents a new microchip which can separate motile sperm by chemotaxis. The microchip was developed to create longitudinal concentration gradient in the microchannel due to diffusion. Linearly good concentration gradient of chemoattractant was generated without any fluid control devices. In sperm separation experiment with the developed microchip, mouse sperm was used as sample and acetylcholine was selected as chemoattractant. Human tubal fluid (HTF), buffer solution, was introduced into the microchannel of the microchip and attractants diluted in ratio of 1, 1/2, 1/4, 1/8, 1/16, 1/32 and 1/64 including control (DI water) were dropped in each outlet by $2\;{\mu}l$ volume with micropippet. After 5min, $1\;{\mu}l$ sperm solution was dropped into inlet of the chip. After 10 min, when sperms reached to the outlet by chemotaxis, we counted sperms in each outlet by using microscopy. Consequently, we could separate progressive motile sperm with the new microchip. In the experiment, the most sperms were isolated at the outlet dropped with 1/16 diluted solution. The optimal concentration gradient to induce chemotaxis was about 0.625 mg/ml/mm.

키워드

참고문헌

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