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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation of lysozyme producing bacteria capable of solubilizing microbial cells

미생물 용해가 가능한 Iysozyme 분비 균주의 분리 및 특성

  • Guo, Pengfei (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Seo, Sun-Keun (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Zhang, Lei (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Kim, Hyo-Sang (GS E&C Research Institute) ;
  • Oh, Young-Khee (GS E&C Research Institute) ;
  • Jahng, Deok-Jin (Department of Environmental Engineering and Biotechnology, Myongji University)
  • Published : 2008.06.30
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Abstract

Lysozyme-producing microorganisms were isolated to obtain bacteria which can efficiently solubilize microbial cells. Cells of normal and chloroform-treated Escherichia coli and Micrococcus Iysodeikticus were used as model substrates to isolate lysozyme-producing microorganisms and investigate the efficiency of cell lysis. The culture supernatant of the isolate New1 (98% similarity of 16S rDNA sequence with Thermomonas haemolytica) showed different lytic characteristics for different substrates. Thermal treatment (autoclave) of substrate cells showed a significant effect on cell solubilization by culture supernatant of the New1. For autoclaved substrate cells, E. coli, M. Iysodeikticus and chloroform-treated E. coli were solubilized by 58.7%, 49.4% and 79.1%, respectively, in the culture supernatant of New1. The lytic activity of New1 was mainly caused by lysozyme produced by the isolate. It was also showed that New1 exhibited high protease activity and a little cellulase activity.

Keywords

References

  1. Akesson, P., SjOholm, A. G., and BjOrck, L. (1996), Protein SIC, a novel extracellular protein of Streptococcus pyogenes interfering with complement function. J Biol Chem 271, 1081- 1088 https://doi.org/10.1074/jbc.271.2.1081
  2. Binks, M. J., Fernie-King, B. A., Seilly, D. J., Lachmann, P. J., and Sriprakash, K. S. (2005), Attribution of the various inhibitory actions of the streptococcal inhibitor of complement (SIC) to regions within the molecule. J Biol Chem 280, 20120-20125 https://doi.org/10.1074/jbc.M414194200
  3. Davis, R. D. and Hall, J. E. (1997) Production, treatment and disposal of wastewater sludge in Europe from a UK perspective. Eur Water Pollut Control 7, 9-17
  4. Dean, C. R. and Ward, O. P. (1991) Nature of Escherichia coli cell lysis by culture supernatants of Bacillus species. Appl Environ Microbiol 57, 1893-1898
  5. Deleris, S., Paul, E., Audic, J. M., Roustan, M., and Debellefontaine, H. (2000), Effect of ozonation on activated sludge solubilization and mineralization. Ozone Sci Eng 22, 473-486 https://doi.org/10.1080/01919510009408791
  6. Duewel, H. S., Daub, E., and Honek, J. F. (1995), Investigations of the interactions of saccharides with the lysozyme from bacteriophage lambda. Biochirn Biophys Acta 1247, 149-158 https://doi.org/10.1016/0167-4838(94)00207-W
  7. Gessesse, A., Dueholm, T., Petersen, S. B., and Nielsen, P. H. (2003) Lipase and protease extraction from activated sludge. Water Res 37, 3652-3657 https://doi.org/10.1016/S0043-1354(03)00241-0
  8. Ghose, T. K. (1987) Measurement of cellulase activities. Pure Appl Chem 59, 257-268 https://doi.org/10.1351/pac198759020257
  9. Goel. R., Mino. T., Satoh. H., and Matsuo. T. (1998), Enzyme activities under anaerobic and aerobic conditions in activated sludge sequencing batch reactor. Water Res 32, 2081-2088 https://doi.org/10.1016/S0043-1354(97)00425-9
  10. Hasegawa, S., Shiota, N., Katsura, K., and Akashi, A. (2000), Solubilization of organic sludge by thermophilic aerobic bacteria as a pretreatment for anaerobic digestion. Water Sci Technol 41, 163-169
  11. Hoefel, D., Monis, P. T., Grooby, W. L., Andrews, S., Saint, C. P. (2005), Culture-independent techniques for rapid detection of bacteria associated with loss of chloramine residual in a drinking water system. Appl Environ Microbiol 71, 6479-6488 https://doi.org/10.1128/AEM.71.11.6479-6488.2005
  12. Iandolo, J. J. and Ordal, Z. J. (1966), Repair of thermal injury of Staphylococcus aureus. J Bacteriol 91, 134-142
  13. Li, Y. Y. and Noike, T. (1992), Upgrading of anaerobic digestion of waste activated sludge by thermal pretreatment. Water Sci Technol 26, 857-866 https://doi.org/10.2166/wst.1992.0466
  14. Mao, T., Hong, S. Y., Show, K. Y., Tay, J. H., and Lee, D. J. (2004), A comparison of ultrasound treatment on primary and secondary sludges. Water Sci Technol 50, 91-97
  15. Middelberg, A. P. (1995), Process-scale disruption of microorganisms. Biotechnol Adv 13, 491-551 https://doi.org/10.1016/0734-9750(95)02007-P
  16. Mori, T. (1995), Treatment of high concentration organic wastewater by thermophilic aerobic digestion. J WatWaste 37, 40-44
  17. Monchois, V., Abergel, C., Sturgis, J., Jeudy, S., and Claverie, J. M. (2001), Escherichia coli ykfE ORFan gene encodes a potent inhibitor of C-type lysozyme. J Biol Chem 276, 18437-18441 https://doi.org/10.1074/jbc.M010297200
  18. Mueller, J. (2000), Disintegration as a key-step in sewage sludge treatment. Water Sci Technol 41, 123-130
  19. Murao, S. and Takahara, G. (1974), Enzymes lytic agaiffit Pseudomonas aeruginosa produced by Bacillus subtilis YT-25. Agric Biol Chem 38, 2305-2316 https://doi.org/10.1271/bbb1961.38.2305
  20. Nakimbugwe, D., Masschalck, B., Deckers, D., Callewaert, L., Aertsen, A., and Michiels, C. W. (2006), Cell wall substrate specificity of six different Iysozymes and lysozyme inhibitory activity of bacterial extracts. FEMS Microbiol Lett 259, 41-46 https://doi.org/10.1111/j.1574-6968.2006.00240.x
  21. Pin, C., Marin, M. L., Selgas, D., Garcia, M. L., Tormo, J., and Casas, C. (1995), Differences in production of several extracellular virulence factors in clinical and food Aeromonas spp. strains. J Appl Bacteriol 78, 175-179 https://doi.org/10.1111/j.1365-2672.1995.tb02839.x
  22. Ren, X., Yu, D., Yu, L., Gao, G., Han, S., and Feng, Y. (2007), A new study of cell disruption to release recombinant thermostable enzyme from Escherichia coli by thermolysis. J Biotechnol 129, 668-673 https://doi.org/10.1016/j.jbiotec.2007.01.038
  23. Rocher, M., Roux, G., Goma, G., Begue, A. P., Louvel, L., and Rols, J. L. (2001), Excess sludge reduction in activated sludge processes by integrating biomass alkaline heat treatment. Water Sci Technol 44, 437-444 https://doi.org/10.2166/wst.2001.0799
  24. Sambrook, J., Fritsch, E. E, and Maniatis, T. (1989), Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
  25. Spellman, F. R. (1997), Wastewater biosolids to compost. Lancaster, Technomic Publishing Company, Lancaster, PA, USA.
  26. Ugwuanyi, J. O., Harvey, L. M, and McNeil, B. (2004), Development of thermophilic populations, amylase and cellulase enzyme activities during thermophilic aerobic digestion of model agricultural waste slurry. Process Biochem 39, 1661-1669 https://doi.org/10.1016/S0032-9592(03)00309-1
  27. Weemaes, M. P. J. and Verstraete, W. H. (1998), Evaluation of current wet sludge disintegration techniques. J Chem Technol Biotechnol 73, 83-92 https://doi.org/10.1002/(SICI)1097-4660(1998100)73:2<83::AID-JCTB932>3.0.CO;2-2
  28. White, M. D. and Marcus, D. (1988) Disintegration of microorganisms. Adv Biotechnol Processes 8, 51-96
  29. Whiteley, C. G., Burgess, J. E., Melamane, X., Pletschke, B., and Rose, P. D. (2003), The enzymology of sludge solubilisation utilising sulphate-reducing systems: the properties of lipases. Water Res 37, 289-296 https://doi.org/10.1016/S0043-1354(02)00281-6