International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Inhibition of osteoclast formation by putative human cementoblasts

  • Kim, Mi-Ri (Department of Dentistry, Asan Medical Center, University of Ulsan) ;
  • Yang, Won-Kyung (Department of Dentistry, Asan Medical Center, University of Ulsan) ;
  • Grzesik, Wojciech (Department of Anatomy and Cell Biology, School of Dental Medicine, University of Pennsylvania) ;
  • Ko, Hyun-Jung (Department of Dentistry, Asan Medical Center, University of Ulsan)
  • Published : 2008.09.30
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Abstract

Cementum is the mineralized tissue of the tooth. It is similar to bone in several aspects but it differs from bone. Human bone marrow stromal cells (BMSC) and human cementum derived cells (HCDC) (10,000 $cells/cm^2$) were plated in 6 well plates as feeder cells. The next day, mouse bone marrow cells (1.5 million $cells/cm^2$) were added. One group of these plates were incubated in serum-free conditioned medium (SFCM) generated from BMSC or HCDC supplemented with 2% FBS, parathyroid hormone (PTH), 1, 25 dihydroxyvitamin $D_3$ (Vit. $D_3$) and dexamethasone, or plain medium with the same supplements. Another group of plates were cocultured with BMSC or HCDC in plain medium supplemented with 2% FBS, PTH, Vit. $D_3$ and dexamethasone. Plates grown without SFCM or coculture were used as controls. After 10 days, the cells were stained for tartrate-resistant acid phosphatase (TRAP). BMSC were found to support osteoclast formation under normal conditions. This was inhibited however by both SFCM generated from HCDC and also by coculture with HCDC. In addition, HCDC themselves did not support osteoclast formation under any conditions. Our results thus indicate that HCDC do not support osteoclast formation in vitro and that soluble factor (s) from HCDC may inhibit this process. In addition, we show that this inhibition also involves an active mechanism that is independent of osteoprotegerin, a feature that may distinguish cementoblasts from other cells present in periodontium.

Keywords

References

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