International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Osteogenic Differentiation Potential in Parthenogenetic Murine Embryonic Stem Cells

  • Kang, Ho-In (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry) ;
  • Cha, Eun-Sook (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry) ;
  • Choi, Young-Ju (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry) ;
  • Min, Byung-Moo (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry) ;
  • Roh, Sang-Ho (Department of Craniomaxillofacial Reconstructive Science Major, Dental Research Institute, and CLS21, Seoul National University School of Dentistry)
  • Published : 2008.09.30
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Abstract

Embryonic stem cells have a pluripotency and a potential to differentiate to all type of cells. In our previous study, we have shown that embryonic stem cells (ESCs) lines can be generated from murine parthenogenetic embryos. This parthenogenetic ESCs line can be a useful stem cell source for tissue repair and regeneration. The defect in full-term development of parthenogenetic ESCs line enables researchers to avoid the ethical concerns related with ESCs research. In this study, we presented the results demonstrating that parthenogenetic ESCs can be induced into osteogenic cells by supplementing culture media with ascorbic acid and $\beta$-glycerophosphate. These cells showed morphologies of osteogenic cells and it was proven by Von Kossa staining and Alizarin Red staining. Expression of marker genes for osteogenic cells (osteopontin, osteonectin, alkaline phosphatase, osteocalcin, bone-sialoprotein, collagen type1, and Cbfa1) also confirmed osteogenic potential of these cells. These results demonstrate that osteogenic cells can be generated from parthenogenetic ESCs in vitro.

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References

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