Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
권호 표지

Genetic Differentiation of the Largemouth Bass Micropterus salmoides from the Major Rivers and Reservoirs in Korea Assessed by AFLP

우리나라 주요 강과 호수에 분포하는 외래어종 배스 Micropterus salmoides의 AFLP 분석에 의한 유전적 분화

  • Lee, Wan-Ok (Central Inland Fisheries Research Institute, NFRDI) ;
  • Lee, Il-Ro (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Song, Ha-Yoon (Department of Marine Biotechnology, Soonchunhyang University) ;
  • Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
  • 이완옥 (국립수산과학원 중부내수면연구소) ;
  • 이일로 (순천향대학교 해양생명공학과) ;
  • 송하윤 (순천향대학교 해양생명공학과) ;
  • 방인철 (순천향대학교 해양생명공학과)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Genetic diversity and differentiation within or among nine populations of introduced fish, largemouth bass Micropterus salmoides were assessed by AFLP. The AFLP analysis using three primer combinations generated 299.2. AFLP bands and percentage of polymorphic bands were similar in those nine populations, ranging 14.1 to 21%. Heterozygosity and genetic diversity within or among populations were quite low for all of these populations with average values ranging from 0.054 to 0.067 and from 0.069 to 0.085, respectively. Analyses of pairwise distance and genetic similarity among nine populations of Micropterus salmoides also revealed the similar results with low genetic differentiation one another. Although pairwise Fst values were low, they were indicated a clear distinct genetic differentiation among the nine populations. These results indicate that very small population of the largemouth bass was first introduced to Paldang reservoir and they are widely spread at most of aquatic habitats in Korea.

한국에 도입된 외래어종 배스 Micropterus salmoides 9 집단의 유전 다양성 및 집단구조를 AFLP 분석을 통해 조사하였다. 3쌍의 primer 조합을 이용한 AFLP 분석에서 총 299.2개의 밴드가 생성되어 다형성 밴드의 비율은 14.1$\sim$21%로 유사하게 나타났으며, 이형접합률 (0.054$\sim$0.067) 및 유전적 다양성 (0.069$\sim$0.085)은 낮은 값을 보였다. 집단간 유전적 거리 및 상동성 분석 역시 유사한 결과를 나타내어 본 연구에 사용된 배스 9집단은 유전적으로 매우 밀접한 근연관계를 나타내었다. 또한 집단간 분화도가 비교적 높게 나타나 9집단의 유전적 분화가 빠르게 진행 중인 것으로 나타났다. 따라서 배스 집단의 낮은 유전 다양성과 집단간 분화도 값은 매우 작은 집단이 팔당호에 처음 도입되어 우리나라 전 수계에 확산되었음을 암시한다.

Keywords

References

  1. 국립수산진흥원. 2001. 국립수산진흥원 80년사. 동양칼라인쇄, 부산, 611p
  2. 환경부. 2008. 야생동식물보호법. 환경부. http://www.me.go.kr/kor/statute/statute_04.jsp
  3. 홍영표. 2002. 국내 외래어종의 분포와 확산경로 및 조절대책. 한국어류학회 심포지엄, p. 27-43
  4. Aoki D., Y. Nakayama, M. Hayashi and G. Iwasaki. 2006. Genetic diversity and origin of Florida largemouth bass (Micropterus salmoides floridanus) introduced into Lake Biwa inferred from the mitochondrial DNA control region. Jap. J. Conserv. Ecol. 11: 53-60
  5. Asahida, T., T. Kobayashi, K. Saitoh and I. Nakayama. 1996. Tissue preservation and total DNA extraction from fish stored at ambient temperature using buffers containing high concentration of urea. Fish. Sci. 62: 727-730 https://doi.org/10.2331/fishsci.62.727
  6. Azuma, M. 1992. Ecological release in feeding behavior: the case of Bluegills in Japan. Hydrobiol. 244: 269-276 https://doi.org/10.1007/BF00007042
  7. Brown, J.H. and M.V. Lomolino. 1998. Biogeography. Sunderland, MA: Sinauer, 624p
  8. Gozlan, R.E., S. St-Hilaire, S.W. Feist, P. Martin and M.L. Kent. 2005. Disease threat to European fish. Nature 435: 1046 https://doi.org/10.1038/4351046a
  9. Hong, Y.P. and Y.M. Son. 2003. Studies on the interspecific association of community including Micropterus salmoides population, introduced fish in Korea. Korean J. Ichthyol. 15: 61-68. (in Korean)
  10. Jang, M.-H. 2002. Ecological study of freshwater fish in Korea : Fish fauna, prey-predator interaction and the responses of cyanobacteria to fish grazing. Ph. D. dissertation, Pusan National Univ. Busan, 224p
  11. Jang, M.-H., G.-J. Joo and M.C. Lucas. 2006. Diet of introduced largemouth bass in Korean rivers and potential interactions with native fishes. Ecol. Freshwater Fish 15(3): 315-320 https://doi.org/10.1111/j.1600-0633.2006.00161.x
  12. Jang, M.-H., J.-G. Kim, S.-B. Park, K.-S. Jeong, G.-I. Cho and G.-J. Joo. 2002. The current status of the distribution of introduced fish in large river systems of South Korea. Internat. Rev. Hydrobiol. 87(2-3): 319-328 https://doi.org/10.1002/1522-2632(200205)87:2/3<319::AID-IROH319>3.0.CO;2-N
  13. Japanese Ministry of the Environment. 2005. The Invasive Alien Species Act. http://www.env.go.jp/nature/intro/3shiryou.html
  14. Lee, I.-R., Y.-A. Lee, H. Shin, Y.K. Nam, W.-J. Kim and I.-C. Bang. 2008a. Genetic diversity of an endangered fish, Iksookimia choii (Cypriniformes), from Korea as assessed by amplified fragment length polymorphism. Korean Journal of Limnology 41: 97-102. (in Korean)
  15. Lee, Y.-A., Y.-E. Yun, Y.K. Nam and I.-C. Bang. 2008b. Genetic diversity of endangered fish Hemibarbus mylodon (Cyprinidae) assessed by AFLP. J. Aquaculture 21(3): 196-200
  16. Maezono, Y. and T. Miyashita. 2002. Community-level impacts induced by introduced largemouth bass and bluegill in farm ponds in Japan. Biol. Conserv. 109: 111-121
  17. Mariette, S., D. Chagne, C. Lezier, P. Pastuszka, A. Raffin, C. Plomion and A. Kremer. 2001. Genetic diversity within and among Pinus pinaster populations: comparison between AFLP and microsatellite marker. J. Hered. 86: 469-479 https://doi.org/10.1046/j.1365-2540.2001.00852.x
  18. McDowall, R.M. 1984. Exotic fishes-the New Zealand experience. In Courteny, W.R. and J.R. Stauffer (eds), Distribution, Biology and Management of Exotic Fishes. John Hopkins University Press, Baltimore, p. 200-214
  19. Miller, M. 1997. Tools for population genetic analysis (TFPGA) 1.3: a windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. http://www.Marksgenetic software.net/tfpga.htm
  20. Mueller, U.G. and L.L. Wolfenbarger. 1999. AFLP genotyping and fingerprinting. Trends in Ecol. Evol. 14: 389-394 https://doi.org/10.1016/S0169-5347(99)01659-6
  21. Nei, M. and W.H. Li. 1979. Mathematical model for studing genetical variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 70: 3321-3323
  22. Rohlf, S.B. 1992. NTSYS-pc numerical taxonomy and multivariate analysis system, version 1.7. Applied Biostatistics Inc. New York, USA
  23. Stefan, S., D. Roessli and L. Excoffier. 2000. Arlequin ver. 2.000: A software for population genetics data analysis. Genetics and Biometry Laboratory, Univ. of Geneva
  24. Takamura, K. 2007. Performance as a fish predator of largemouth bass Micropterus salmoides (Lacepede) invading Japanese freshwaters: a review. Ecol. Res. 22: 940-946 https://doi.org/10.1007/s11284-007-0415-7
  25. Terashima, A. 1980. Bluegill: a vacant ecological niche in Lake Biwa. In: T. Kawai, H. Kawanabe and N. Mizuno, Editors, Freshwater Organisms in Japan: Their Ecology of Invasion and Disturbance, Tokai Univ. Press, Tokyo, p. 63-70
  26. Vos, P., R. Hodgers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau. 1995. AFLP, a new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407-4414 https://doi.org/10.1093/nar/23.21.4407
  27. Wright, S. 1951. The genetic structure of populations. Ann. Eugen. 15: 323-354
  28. Wright, S. 1978. Evolution and the genetics of populations; Vol 4: Variability within and among natural populations. Univ. of Chicago Press, Chicago
  29. Yodo, T. and S. Kimura. 1998. Feeding habits of largemouth bass Micropterus salmoides in lakes Shorenji and Nishinoko, Central Japan. Nippon Suisan Gakkaishi 64: 26-28. (in Japanese) https://doi.org/10.2331/suisan.64.26
  30. Yodo, T. and S. Kimura. 2002. Gonadal maturation of largemouth bass Micropterus salmoides in lakes Shorenji and Nishinoko, Central Japan. Nippon Suisan Gakkaishi 68: 151-156. (in Japanese) https://doi.org/10.2331/suisan.68.151