Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-microbial Activity of Korean Fermented Soybean Paste (Doen-jang) Against Oral Microbes

한국 전통 된장의 구강미생물에 대한 항균효과

  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was performed to investigate the anti-microbial activity of extract from Korean fermented soybean paste (doen-jang) against 16 types of oral microbes, and to determine the minimum inhibition concentration (MIC) of the extract for three major microbes causing human oral diseases (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans). The extract was prepared using ethyl acetate and it was treated with the oral microbes at a concentration of 5.00 mg/ml (0.5%). The anti-microbial activity and MIC were measured using broth dilution method. Significant reduction of microbial activities of 16 types of oral microbes occurred when the soybean paste extract was added to the broth compared to the control (p<0.01), and striking inhibition (more than 99%) was observed in ten types. S. mutans, which causes dental caries, showed MIC at a concentration of 1.25 mg/ml for the extract. P. gingivalis, which causes adult periodontal disease, showed MIC at a concentration of 2.50 mg/ml for the extract. C. albicans, which causes denture stomatitis and angular stomatitis, showed MIC at a concentration of 20 mg/ml for the extract. These results indicate that ethyl acetate extract of doen-jang showed strong anti-microbial effect against 16 types of oral microbes, and the anti-microbial effect of the extract against oral microbes was stronger against bacteria than against fungi. The anti-microbial effect might be possibly enhanced by the fermentation of soybeans.

Keywords

References

  1. Han, M. D. and Kim, Y. K. : Oral microbiology, Komoonsa Publishing Co., Seoul, 216, 1998
  2. Gibbons, R. J. and Hay, D. I. : Adsorbed salivary acidic proline-rich proteins contribute to the adhesion of Streptococcus mutans JBP to apatitic surfaces. Journal of Dental Research, 68, 1303-1307, 1989 https://doi.org/10.1177/00220345890680090201
  3. Kozai, K. Y., Miyake, H., Kohds, S., Kamataka, K., Yamasaki, H., Suginaka, H. and Nagasaka, K. : Inhibition of glucosyltransferase from Streptococcus mutans by oleanolic acid and urosonic. Caries Research, 21, 104-108, 1987 https://doi.org/10.1159/000261010
  4. Park, J. H., Keum, K. Y., Lee, J. H., Yu, J. Y. and Lee, S. J. : The inhibitory effect of taurine and alendronate on the osteoceoclast differentiation mediated by sonicated extr acts o f Porphyromonas gingivalis in vitro. Journal of Korean Academy of Operative Dentistry, 26(4), 285-295, 2001
  5. Kim, Y. H., Kang, T. S., Kim, S. H., Min, B. H., Yang, B. S. and Choi, W. C. : Diagnostic microbiology. Hyunmoonsa Publishing Co., Seoul, 803, 2002
  6. Heimdahl, E. N., Nord, C. E. and Sviuhufvu, L. B. : Effect of topical administration of vancomycin versus chlorhexdine on $\alpha$-hemolytic streptococci in the oral cavity. Oral Surgery, Oral Medicine, Oral Pathology, 66, 304-309, 1988 https://doi.org/10.1016/0030-4220(88)90236-8
  7. Ullsfoss, B. N., Ogaard, B. and Arends, J. : Effect of a combined chlorohexdine and NaF mouth-rinse: an in vivo human caries model study. Scandinavian Journal of Dental Research, 102, 109-112, 1994
  8. Jeon, H. C. and Kim, C. C. : Inhibitory effect of fluoride and chlorohexidine mouthrinses on S. mutans and Lactobacillus in preschool children with nursing caries. Journal of the Korean Aacdemy of Pediatric Dentistry, 22(1), 15-26, 1995
  9. Nam, Y. H., Kim, K. C. and Lee, K. H. : A study on the influences of salivary and Streptococcus mutans and calcium on dmft. Korean Journal of Pediatrics, 17(1), 55-64, 1990
  10. Kim, J. I. : Analysis of health factor for centenarians in Korea. Health Welfare, 1, 9-38, 1998 https://doi.org/10.23948/kshw.1998.12.1.9
  11. Lee, I. K. and Kim, J. G. : Effects of dietary supplementation of Korean soybean paste(doenn-jang) on th lipid metabolism in rats fed a high fat and/or a high cholesterol diet. Journal of Korean Public Health Association, 28, 282-305, 2002
  12. Kim, J. G., Lee, Y. W., Kim, P. G., Roh, W. S. and Shintani, H. : Reduction of aflatoxins by Korean soybean paste and its effect on cytotoxicity and reproductive toxicity-part 1. Journal of Food Protection, 63(9), 1295-1298, 2000
  13. Choi, S. M., Cheigh, M. J. and Lee, J. J. : Growth suppression effect of traditional fermented soybean paste (doenjang) on the various tumor cells. Journal of Korean Society of Food Science and Nutrition, 28, 458-463, 1999
  14. Kim, J. G. : Antigenotoxic effects of water extract from Korean fermented soybean paste(doen--jang). Journal of Food Protection, 61(1), 156-161, 2004
  15. Lee, S. L. and Kim, J. G. : Inhibitory effects of doenjang (Korean fermented soybean paste) and soybean extracts on the growth of KB cells. Korean Journal of Environmental Health, 31(5), 444-450, 2005
  16. Korea Food Research Institute : Recommended method of preparing korean soybean paste, Korea Food Research Institute, Sung-nam, Korea, 1994
  17. Kim, J. G., Lee, Y. W. and Bullerman, L. B. : Changes of aflatoxins during the ripening and storage of Korean soy sauce and soy paste and the characteristics of the changes-part 3. Korean Journal of Public Health, 1, 21-28, 2000
  18. Laura, J. and Piddock, V. : Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria. Journal of Applied Bacteriology, 68, 307-318, 1990 https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
  19. Lee, S. L. and Kim, J. G. : Antimicrobial activity of soybean extract against oral microbes. Korean Journal of Environmental Health, 32(2), 192-197, 2006
  20. Kang, K. S. : Antibacterial effect of polyphosphate on Mutans streptococci, Doctorate dissertation of Kyunghee University, 2001
  21. Choi, I. S. : Antibacterial effect of polyphosphate on Porphyromonas gingivalis, Doctorate dissertation of Kyunghee University, 1998
  22. Kim, T. J. : Antibacterial effect of polyphosphate on Candida albicans, Master's degree thesis of Kyunghee University, 2002
  23. Kong, H. J., Choi, H. Y., Min, B. S., Park, S. J., Lee, J. Y. and Choi, G. W. : Effect of polyphosphate on the growth of oral bacterium. Journal of Korean Academy of Operative Dentistry, 23(2), 550-560, 1998

Cited by

  1. The Quality Characteristics of Commercial Deonjang Certified for Traditional Foods vol.24, pp.4, 2013, https://doi.org/10.7856/kjcls.2013.24.4.537
  2. Development and Validation of On-line Column Switching HPLC-MS/MS Method for 10 Phthalate Metabolites in Human Urine vol.36, pp.6, 2010, https://doi.org/10.5668/JEHS.2010.36.6.510