International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

High-level Expression, Polyclonal Antibody Preparation and Bioinformatics Analysis of Bombyx mori Nucleopolyhedrovirus orf47 Encodes Protein

  • Wu, Chao (Institute of Life Sciences, Jiangsu University) ;
  • Guo, Zhongjian (Institute of Life Sciences, Jiangsu University) ;
  • Chen, Keping (Institute of Life Sciences, Jiangsu University) ;
  • Shen, Hongxing (Institute of Life Sciences, Jiangsu University)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.

Keywords

References

  1. Acharya, A. and K. Gopinathan (2002). "Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus." Virology 299(2), 213- 24. https://doi.org/10.1006/viro.2002.1525
  2. Ayres, M. D., S. C. Howard, J. Kuzio, M. Lopez-Ferber and R. D. Possee (1994). "The complete DNA sequence of Autographa californica nuclear polyhedrosis virus." Virology 202(2), 586-605. https://doi.org/10.1006/viro.1994.1380
  3. Baluchamy, S. and K. P. Gopinathan (2005). "Characterization of a cyclin homolog from Bombyx mori nucleopolyhedrovirus." Virus Res 108(1-2): 69-81. https://doi.org/10.1016/j.virusres.2004.08.004
  4. Chen, H. Q., K. P. Chen, Q. Yao, Z. J. Guo and L. L. Wang (2007). "Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus." FEBS Lett 581(30), 5836- 42. https://doi.org/10.1016/j.febslet.2007.11.059
  5. Daimon, T., S. Katsuma and T. Shimada (2007). "Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus." Virus Res 124(1-2), 168-75. https://doi.org/10.1016/j.virusres.2006.11.001
  6. Du, M. F., X. M. Yin, Z. J. Guo and L. J. Zhu (2006). "Characterization of a late gene, ORF60 from Bombyx mori nucleopolyhedrovirus." J Biochem Mol Biol 39(6), 737-42. https://doi.org/10.5483/BMBRep.2006.39.6.737
  7. Gomi, S., K. Majima and S. Maeda (1999). "Sequence analysis of the genome of Bombyx mori nucleopolyhedrovirus." J Gen Virol 80(Pt 5), 1323-37. https://doi.org/10.1099/0022-1317-80-5-1323
  8. Herniou, E. A., T. Luque, X. Chen, J. M. Vlak, D. Winstanley, J. S. Cory and D. R. O'Reilly (2001). "Use of whole genome sequence data to infer baculovirus phylogeny." J Virol 75(17), 8117-26. https://doi.org/10.1128/JVI.75.17.8117-8126.2001
  9. Hu, Y. X., J. Y. Guo, L. Shen, Y. Chen, Z. C. Zhang and Y. L. Zhang (2002). "Get effective polyclonal antisera in one month." Cell Res 12(2), 157-60. https://doi.org/10.1038/sj.cr.7290122
  10. Imai, N., M. Kurihara, S. Matsumoto and W. K. Kang (2004). "Bombyx mori nucleopolyhedrovirus orf8 encodes a nucleic acid binding protein that colocalizes with IE1 during infection." Arch Virol 149(8), 1581-94.
  11. Jehle, J. A., G. W. Blissard, B. C. Bonning, J. S. Cory, E. A. Herniou, G. F. Rohrmann, D. A. Theilmann, S. M. Thiem and J. M. Vlak (2006). "On the classification and nomenclature of baculoviruses: a proposal for revision." Arch Virol 151(7), 1257-66. https://doi.org/10.1007/s00705-006-0763-6
  12. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, second ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
  13. Katsuma, S., S. Horie, T. Daimon, M. Iwanaga and T. Shimada (2006). "In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf." Virology 355(1), 62-70. https://doi.org/10.1016/j.virol.2006.07.008
  14. Lu, M., L. Swevers and K. Iatrou (1998). "The p95 gene of Bombyx mori nuclear polyhedrosis virus: temporal expression and functional properties." J Virol 72(6), 4789-97.
  15. Park, K. J. and M. Kanehisa (2003). "Prediction of protein subcellular locations by support vector machines using compositions of amino acids and amino acid pairs." Bioinformatics 19(13), 1656-63. https://doi.org/10.1093/bioinformatics/btg222
  16. Shijuku, T., T. Yamashino, H. Ohashi, H. Saito, T. Kakegawa, M. Ohta and H. Kobayashi (2002). "Expression of chaA, a sodium ion extrusion system of Escherichia coli, is regulated by osmolarity and pH." Biochim Biophys Acta 1556(2-3), 142-8. https://doi.org/10.1016/S0005-2728(02)00345-6
  17. Wang, F., L. R. Yang, X. D. Tang, J. C. Mo, W. J. Yang and C. X. Zhang (2006). "The translational and transcriptional initiation sites of BmNPV lef-7 gene." Virus Genes 33(3), 351-7.
  18. Xu, H. J., Z. N. Yang, F. Wang and C. X. Zhang (2006). "Bombyx mori nucleopolyhedrovirus ORF79 encodes a 28-kDa structural protein of the ODV envelope." Arch Virol 151(4), 681-95. https://doi.org/10.1007/s00705-005-0664-0