Ultrastructural Changes In the Midgut During Metamorphosis in Apis Cerana Indica.

  • Published : 2008.03.31

Abstract

The midgut epithelium of Indian honey bee Apis cerana indica is consist of digestive cells and small regenerative cells. The regenerative cells are placed in the nests scattered among the digestive cells. During metamorphosis the midgut of Apis cerana indica is remodeled. The larval midgut epithelium and muscular sheath digested partially at the end of larval period and thrown out in the form of debris in the lumen. The new epithelium is formed by the proliferation of the regenerative cells and during pupation reorganization of midgut layer occurs. The ultrastuctural studies shows that the regenerative cells are in contact with degenerative cells by the cytoplasmic extension which have many septed and gap junctions in the fifth instar larvae. In developing pupae reorganization of the midgut epithelium is continued whereas in the pharate adult the midgut wall shows, characteristic of adult midgut epithelium with pycnotic nuclei in some cells.

Keywords

References

  1. CHAPMAN, R. F. (1985a) Co-ordination of digestion. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Eds. G.A. Kerkut and L. I. Gilbert) 4, 213-240. Oxford. Pergamon Press
  2. CHAPMAN, R. F. (1985b) Structure of digestive system. In Comprehensive Insect Physiology Biochemistry and Pharmacology (Eds. G. A. Kerkut and L. I. Gilbert) 4, 165-211
  3. CRAILSHEM AND M. A. PABST (1990) Scanning electron microscopic studies of the proventriculus of honeybee worker, drones and queens correlation with feeding behaviour. In social insects and the environment. (Proc. 11th Internat. Cong. JUSSI. India. Eds. Veeresh, G. K. Malik, B. and Viraktamath, C. A.). Oxford and IBH Publ. Co. Pvt. Ltd., New Delhi. 124
  4. CRUZ-LANDIM, C. AND V. M. CAVALCANTE (2003) Ultrastructural and cytochemical aspects of metamorphosis in the midgut of Apis mellifera L. (Hymenoptera: Apidae: Apinae)
  5. CRUZ-LANDIM, C (1985) Origin of the peritrophic membrane of the adult Apis mellifera L. (Hymenoptera : Apidae). Rev, Brasil Biol. 45, 207-219
  6. DE PRIESTER, W. (1971) Ultrastructure of midgut epithelial cells in the fly, Calliphhora erythrocephala. J. Ultrastru. Res. 36, 783-805 https://doi.org/10.1016/S0022-5320(71)90031-1
  7. GREGORC, A. AND I. D.BOWEN (1997) Programmed cell death in the honey-bee (Apis mellifera L.) larvae midgut. Cell. Biol. Int. 21(3), 151-158 https://doi.org/10.1006/cbir.1997.0127
  8. HUMASON, G.L. (1962) Animal Tissue Technique W.H. Freeman and company, san Franscisco And London
  9. JIMENEZ, R. D. AND M. GILLIAM (1990) Ultrastructure of the ventriculus of the honey bee, Apis mellifera (L). Cytochemical localization of acid phosphatase, alkaline phosphasphatase, and nonspecific esterase. Cell Tissue Res. 261, 431-443 https://doi.org/10.1007/BF00313521
  10. MARTINS, M. D.,J. F. KINNEAR., and J. A. RHOMSON (1969) Developmental changes in the last larva of Callifora styia.Aust.J Bio.Sci. 22, 935-945 https://doi.org/10.1071/BI9690935
  11. MARTINS G. F., C.A. NEVES., L. A. CAMPUS and J. E. SERRAO (2006) The regenerative during the metamorphosis in the midgut of bees. Microns 37(2), 161-168 https://doi.org/10.1016/j.micron.2005.07.003
  12. RACHINSKY, A., C. Strambi, A. Strambi and K. Hartfelder (1990) cast and metamorphosis :hemolymph titers of juvenile hormone and ecdysteroides in last instar honeybee larvae. Gen comp Endocrinol. 79, 31-38 https://doi.org/10.1016/0016-6480(90)90085-Z
  13. SNODGRASS, R. E. (1956) Anatomy of honeybee. Vail Ballou Press, Inc. Binghamton New York. 168-200
  14. TATA J. R. (1994) Gene expression during post embryonic development metamorphosis as a model. Proc. Indian NaH Sci. Acad Part B Biol Sci 60, 387-301
  15. TIMANDE, J. P. (2005) Study of the honey bee; Apis florea farb. (Hymentoptera : Apidae) and its apiary, products and their commercial value. Ph. D. thesis, RTM., Nagpur University, Nagpur
  16. WIGGLESWORTH (1965) The principles of insect physiology, Methuen and Co. Ltd., London
  17. WILLE A. (1983) A biology of the stingless bees. Ann. Rev. Entomol 28, 51-61