Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
권호 표지

THE ANALYSIS OF REMINERALIZATION EFFECT IN FLUORIDE VARNISH USING CONFOCAL LASER SCANNING MICROSCOPE

공초점 레이저 주사 현미경을 이용한 불소 바니쉬 재광화 효과의 분석

  • Kwon, Ji-Hoon (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Park, Ho-Won (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Lee, Ju-Hyun (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University) ;
  • Seo, Hyun-Woo (Department of Pediatric Dentistry, Oral Science Research Center, College of Dentistry, Kangnung National University)
  • 권지훈 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 박호원 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 이주현 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소) ;
  • 서현우 (강릉대학교 치과대학 소아치과학교실 및 구강과학연구소)
  • Published : 2008.02.29
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Abstract

It is well established that fluoride products play an important role in the prevention and remineralization of carious lesion. Fluoride varnish is a concentrated topical fluoride and varnishes adhere to tooth surface, permitting prolonged fluoride exposure and uptake. In this study, the artificial initial enamel caries was caused on the sound human enamel and divided 60 specimens into three groups. Group 1 and group 2 were treated with the topical application of fluoride varnish and stored in artificial saliva for 1 and 2 weeks. Group 3 was stored in artificial saliva for 2 weeks, which acted as control group. Changes in mineral contents were analysed with the confocal laser scanning microscope. The following results were obtained: 1. In group 1 and group 2, the total fluorescence of the lesion(TFL) was reduced in remineralized area compared to in demineralized area(p<0.05). 2. The total fluorescence of the lesion of remineralized area was more reduced in group 2 than in group 1(p<0.05). 3. The total fluorescence of the lesion was more reduced in group 2 than in control group(p<0.05). 4. Confocal laser scanning microscope can be used in quantitative analysis of remineralization by fluoride varnish.

본 연구의 목적은 인공적으로 형성한 법랑질 초기 우식 병소를 불소 바니쉬로 재광화하여 그에 따른 무기질의 변화를 공초점 레이저 주사 현미경을 사용하여 정량적으로 비교 평가 하는 것이다. 이 연구에서는 발거된 60개의 치아에 인공적으로 법랑질 초기 우식 부위를 형성한 후, 각 20개씩 3군으로 나누었다. 1군과 2군은 실험군으로 불소 바니쉬 도포후 각각 1주, 2주 동안 인공타액에 저장하여 재광화 했으며, 3군은 대조군으로 2주간 인공타액에 저장하였다. 그 후 공초점 레이저 주사 현미경을 사용하여 관찰한 결과 다음과 같은 결과를 얻었다. 1. 실험군에서 탈회 후 불소 바니쉬를 도포한(재광화) 부위의 형광강도는 탈회 부위보다 각각 통계적 유의성 있게 감소하였다(p<0.05). 2. 불소 바니쉬를 도포하고 인공타액에 2주간 보관한 군이 1주간 보관한 군보다 불소 바니쉬 도포 부위의 형광강도 감소가 통계적으로 유의성 있게 더 크게 나타났다(p<0.05). 3. 불소 바니쉬를 도포하고 2주간 인공타액에 보관한 군은 대조군에 비해 불소 바니쉬 도포 부위의 형광강도 감소가 통계적으로 유의성 있게 더 크게 나타났다(p<0.05). 4. 공초점 레이저 주사 현미경을 통해 불소 바니쉬를 이용한 재광화를 정량적으로 분석할 수 있었다.

Keywords

References

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