Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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A HISTOLOGICAL CHANGES AND CGRP EXPRESSION AFTER EXPOSURE INJURY BY ER:YAG LASER IN DENTAL PULP OF RAT

흰쥐 치수에서 Er:YAG laser에 의한 노출손상에 따른 조직학적 변화 및 CGRP 단백질의 발현

  • Yang, Jae-Ho (Oral Biology Research Institute & Post BK21, College of Dentistry, Chosun University) ;
  • Park, Jong-Tae (Oral Biology Research Institute & Post BK21, College of Dentistry, Chosun University) ;
  • Kim, Kyu-Tag (Oral Biology Research Institute & Post BK21, College of Dentistry, Chosun University) ;
  • Kim, Sang-Bong (Oral Biology Research Institute & Post BK21, College of Dentistry, Chosun University) ;
  • Lee, Nan-Young (Department of Pediatric Dentistry, College of Dentistry, Chosun University) ;
  • Lee, Sang-Ho (Department of Pediatric Dentistry, College of Dentistry, Chosun University) ;
  • Kim, Heung-Joong (Oral Biology Research Institute & Post BK21, College of Dentistry, Chosun University)
  • 양재호 (조선대학교 치과대학 구강생물학연구소 및 2단계 BK21) ;
  • 박종태 (조선대학교 치과대학 구강생물학연구소 및 2단계 BK21) ;
  • 김규탁 (조선대학교 치과대학 구강생물학연구소 및 2단계 BK21) ;
  • 김상봉 (조선대학교 치과대학 구강생물학연구소 및 2단계 BK21) ;
  • 이난영 (조선대학교 치과대학 소아치과학교실) ;
  • 이상호 (조선대학교 치과대학 소아치과학교실) ;
  • 김흥중 (조선대학교 치과대학 구강생물학연구소 및 2단계 BK21)
  • Published : 2008.02.29
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Abstract

The purpose of this study was to investigate early histological changes and calcitonin gene-related peptide (CGRP) expression in the dental pulp of the rat after Er:YAG laser preparation. Occlusal cavities were prepared in the upper first molars using either Er:YAG laser and conventional bur. At 48 hours after cavity preparation, the teeth were processed for hematoxylin-eosin stain and CGRP immunohistochemistry. The results were as follows : 1. The cavity floor by Er:YAG laser preparation was more irregular shape compared with those by bur preparation and there are some cracks in the directions of dentinal tubules. 2. There were more inflammatory cell infiltration and disruption of odontoblast in the dental pulp by Er:YAG laser preparation in comparison with the dental pulp by bur preparation. 3. CGRP expression in the pulp tissue by both Er:YAG laser and bur preparations were increased and higher than in the normal pulp. The expression pattern of CGRP was more strong in the pulp by Er:YAG laser preparation. These results indicate that Er:YAG laser is useful in the operative dentistry such as caries removal and cavity preparation if properly applied.

본 연구는 Er:YAG laser에 의한 치수의 초기 조직반응과 calcitonin gene-related peptide (CGRP) 함유 신경섬유의 발현을 비교 평가하고자 시행하였다. Er:YAG laser와 conventional bur를 이용하여 상악 제1대구치의 교합면에 와동을 형성하였다. 와동형성 후 48시간 후에 조직을 처리하여 hematoxylin-eosin 염색과 CGRP 면역조직화학을 시행한 후 다음과 같은 결과를 얻었다. 1. Conventional bur를 이용하여 삭제한 경우, 상아질 바닥면은 균열이 없는 평면의 균일한 모양을 보였으며, Er:YAG laser의 경우에는 상아질의 면은 bur에 비해서 파동형의 불규칙적인 형태와 바닥면에서 상아세관의 주행방향을 따라 미세한 균열을 보였다. 2. Er:YAG laser에 의해 손상 받은 치수에서는 혈관확장과 노출된 치수의 손상부위를 따라 bur에서 손상 받은 치수보다 더 많은 염증세포의 침윤과 상아모세포층의 파괴를 보였다. 3. 노출손상 후에 치수신경 말단에서 CGRP 발현은 bur와 laser군 모두에서 손상을 받지 않은 정상 치수에 비해서 증가된 양상을 보였으며, 그 증가된 정도는 laser의 경우에서 더 강하였다. 위의 결과들을 종합하면, Er:YAG laser가 치수손상을 최소화하여 적용되면 치아우식 제거와 와동형성과 같은 여러 수복치료 시 유용하게 사용될 수 있으리라 사료된다.

Keywords

References

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