Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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EFFECT OF CALCIUM AND VITAMIN D SUPPLEMENTATION ON BONE FORMATION AROUND TITANIUM IMPLANT IN OSTEOPOROSIS-INDUCED RATS

골다공증 유도 흰쥐에서 칼슘과 비타민 D 섭취가 티타늄 임플랜트 주위의 골 형성에 미치는 영향

  • Lee, Jae-Yeol (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Jeong, Seok-Young (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Shin, Sang-Hun (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Gyoo-Cheon (Dept. of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Kim, Yong-Deok (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Chung, In-Kyo (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University) ;
  • Kim, Uk-Kyu (Dept. of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University)
  • 이재열 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 정석영 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 신상훈 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김규천 (부산대학교 치의학전문대학원 구강해부학교실) ;
  • 김용덕 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 정인교 (부산대학교 치의학전문대학원 구강악안면외과학교실) ;
  • 김욱규 (부산대학교 치의학전문대학원 구강악안면외과학교실)
  • Published : 2008.06.30
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Abstract

Purpose: The purpose of this study was to observe the effect of calcium and vitamin D to the titanium implant osseointegration in the osteoporosisinduced animal model. Material and method: Thirty-two rats, 10 weeks of age, were divided into two groups: experimental group was ingested additional calcium and vitamin D, and a control group was not. Titanium screw implant(diameter, 2.0 mm; length, 3.5 mm; pitch-height 0.4 mm) were placed into tibia of 32 rats, 16 in the control group and 16 in the experimental group. The rats were sacrificed at 1, 2, 4 and 8 weeks after implantation for histopathologic examination, histomorphometric analysis and immunohistochemistry with fibronectin and collagen type I antibody. Result: In histopathological findings, newly formed bone was seen at 2 weeks and became lamellar bone at 4 weeks, and mature trabecullar bone was seen at 8 weeks in experimental group. In control group, thickness of regenerated bone increased till 4 weeks gradually and trabecullar bone was seen at 8 weeks. In histomorphometric analysis, marrow bone density increased significantly in experimental group compared to control group. Fibronectin immunoreactivity was strong at 2 weeks in experimental group and reduced after 4 weeks gradually. But it was maintained continuously from 2 to 8 weeks in control group. Collagen type I immunoreactivity was very strong from 2 to 4 week in experimental group. And the amount of Collagen type I expression was more abundant in experimental group. Conclusion: The results of this study suggest that calcium and vitamin D supplementation promote bone healing around titanium implants in osteoporosis induced animals.

Keywords

References

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