Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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THE EFFECTS OF ${\beta}-TCP$/rhBMP-2 ON BONE FORMATION IN OSTEOBLAST-LIKE CELLS INDUCED FROM BONE MARROW-DERIVED MESENCHYMAL STEM CELLS

골수유래줄기세포에서 분화된 골유사세포에서 ${\beta}-TCP$와 rhBMP-2의 골형성 효과에 관한 연구

  • Choi, Yong-Soo (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University) ;
  • Hwang, Kyung-Gyun (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University) ;
  • Lee, Jae-Seon (Institute of Biomedical Science, College of Medicine, Hanyang University) ;
  • Park, Chang-Joo (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University) ;
  • Shim, Kwang-Sup (Division of Oral and Maxillofacial Surgery / Department of Dentistry, College of Medicine, Hanyang University)
  • 최용수 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 황경균 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 이재선 (한양대학교 의과대학 의생명연구소) ;
  • 박창주 (한양대학교 의과대학 치과학교실 구강악안면외과) ;
  • 심광섭 (한양대학교 의과대학 치과학교실 구강악안면외과)
  • Published : 2008.08.29
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Abstract

The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on ${\beta}-tricalcium$ phosphate (${\beta}-TCP$) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM ${\beta}$-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor ${\kappa}B$ ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-Ⅰ (COL-Ⅰ). There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on ${\beta}-TCP(+/-)$. According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/${\beta}-TCP(-)$ than DOCs/${\beta}-TCP(+)$. According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/${\beta}-TCP(+)$ compared to that of DOCs/${\beta}-TCP(-)$ on rhBMP 10 ng/ml. Our study presented the ${\beta}-TCP$ will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.

Keywords

References

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