Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Potential Allelic Association of Microsatellite Markers on Chromosome 1 with Economic Traits in Korean Native Chicken

한국재래닭 1번 염색체내 초위성체 유전표지를 이용한 경제형질 연관 지역 탐색

  • Kim, H.K. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Oh, J.D. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Kang, B.S. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Park, M.N. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Chae, E.J. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Jung, H.M. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Seo, O.S. (Poulrty Science Division, National Institute of Animal Science, RDA) ;
  • Choe, H.S. (School of Animal Science & Biotechnology, Chonbuk National University) ;
  • Jeon, G.J. (Genomic Informatics Center, Hankyong National University) ;
  • Lee, H.K. (Genomic Informatics Center, Hankyong National University) ;
  • Kong, H.S. (Genomic Informatics Center, Hankyong National University)
  • 김학규 (농촌진흥청 축산과학원 가금과) ;
  • 오재돈 (농촌진흥청 축산과학원 가금과) ;
  • 강보석 (농촌진흥청 축산과학원 가금과) ;
  • 박미나 (농촌진흥청 축산과학원 가금과) ;
  • 채은진 (농촌진흥청 축산과학원 가금과) ;
  • 정한민 (농촌진흥청 축산과학원 가금과) ;
  • 서옥석 (농촌진흥청 축산과학원 가금과) ;
  • 최호성 (전북대학교 동물자원과학부) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 이학교 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소)
  • Published : 2008.06.30
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Abstract

A total of 17 polymorphic microsatellite markers on chromosome 1 were used for allelic association tests with phenotypic traits in Korean native chicken. Chi-square tests were performed to compare the frequencies of individual alleles between the high and the low trait groups. The frequency of allele 123 of MCW0160 showed a significant difference between the high and the low groups in the trait of egg weight (EW). Three markers, namely ADL0234, UMA1.125 and ADL0101, were found to show significant differences in allelic distribution for the trait of the first lay day (FLD). UMA1.117, ADL0020, UMA 1.019, LMA1 and ADL0238 were found to show significant differences in allelic distribution for the trait of body weight (BW). ADL0101 and ADL0238 were found to show significant differences in allelic distribution for the trait of number of egg production(EP). In this study, we identified the QTL for economic traits at around 94 (MCW0160), 151 (ADL0234), 170 (UMA1.125), 225 (UMA1.117), 285 (ADL0020), 387 (UMA1.019), 418 (LMA1), 500 (ADL0101) and 520 (ADL0238) cM on chromosome 1 in Korean native chicken. The results provided a useful guideline for identification of positional candidate gene and marker-assisted selection for economic traits in Korean native chicken.

본 연구는 한국재래닭의 1번 염색체내 존재하는 17개의 MS(microsatellite) marker를 이용하여 경제형질과 관련하여 유의적인 연관성을 가진 지역을 탐색하기 위하여 실시하였다. 1번 염색체내 경제형질과의 유의적인 연관성을 가진 지역을 탐색하기 위하여 분석된 17개의 MS marker를 대상으로 각 marker별 대립 유전자의 최다 출현 빈도를 지닌 두 개의 대립 유전자를 선발하였다. 선발된 각각의 대립 유전자는 각 경제형질별 성적을 바탕으로 고능력 집단과 저능력 집단으로 나누었으며, 두 집단간의 Chi-squire 검정을 통해 경제형질과의 연관성을 확인하였다. 분석된 결과에 따르면 난중의 경우 94 cM에 위치한 MCW0106, 1개의 지역에서 유의적인 연관성이 탐색되었다. 시산일령의 경우, 3개의 지역(ADL0234, UMA 1.125, ADL0101)에서 유의적인 연관성이 탐색되었고, 체중의 경우 6개의 지역(UMA1.117, ADL0020, UMA1.019, LAMP1, ADL0101, ADL0238)에서 유의적인 연관성이 탐색되었으며, 마지막으로 산란수의 경우 2개의 지역(ADL0101, ADL0238)에서 유의적인 연관성을 확인하였다. ADL0101는 시산일령, 체중 그리고 산란수에서의 유의적인 연관성이 확인되었으며, 산란수에서는 두개의 대립 유전자(174, 178) 모두에서 유의적인 연관성이 탐색되었음을 확인하였다.

Keywords

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