Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Effects of Feeding Mixture of Probiotics and Colistin® on Performance and Egg Quality in Laying Hens

생균제 및 Colistin® 복합 첨가제의 급여가 산란계의 생산성과 계란의 품질에 미치는 영향

  • Yun, J.Y. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, J.Y. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Kim, J.S. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Lee, B.K. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Ahn, B.K. (College of Animal Bioscience and Technology, Konkuk University) ;
  • Hwang, Y.B. (Biotechnology Department of CHEILBIO Institute, CHEILBIO Co. Ltd.) ;
  • Kang, S.K. (Biotechnology Department of CHEILBIO Institute, CHEILBIO Co. Ltd.) ;
  • Kim, D.G. (Biotechnology Department of CHEILBIO Institute, CHEILBIO Co. Ltd.) ;
  • Kang, C.W. (College of Animal Bioscience and Technology, Konkuk University)
  • 윤지연 (건국대학교 동물생명과학대학) ;
  • 김재영 (건국대학교 동물생명과학대학) ;
  • 김지숙 (건국대학교 동물생명과학대학) ;
  • 이보근 (건국대학교 동물생명과학대학) ;
  • 안병기 (건국대학교 동물생명과학대학) ;
  • 황용배 ((주) 제일바이오 연구소 생명공학실) ;
  • 강성기 ((주) 제일바이오 연구소 생명공학실) ;
  • 김동건 ((주) 제일바이오 연구소 생명공학실) ;
  • 강창원 (건국대학교 동물생명과학대학)
  • Published : 2008.06.30
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Abstract

This experiment was conducted to evaluate the dietary effects of mixture of probiotics and colistin on laying performance, intestinal microflora and egg yolk cholesterol in laying hens. One hundred sixty 50-wk-old Hy-Line Brown layers were divided into four treatments and fed a commercial diet (Control) or experimental diets containing 0.2% mixture of probiotics or probiotics and colistin mixture (T1, Bacillus subtilis + Aspergillus oryzae + Lactobacillus plantarum; T2, Bacillus subtilis + Aspergillus oryzae; T3, Bacillus subtilis + Aspergillus oryzae+colistin) for 8 wk. No significant differences were found in laying performance and liver weight among the groups. The Haugh unit of treated groups were significantly improved (P<0.05) compared to that of control, but eggshell qualities were not changed by the treatments. The cecal ammonia concentration was significantly decreased in both T1 and T3 groups. The number of coli forms in cecal content and feces were significantly reduced in all treated groups compared to that of Control (P<0.05). The egg yolk cholesterol contents in the groups fed the diet containing mixture of probiotics and colistin were reduced in comparison with that of Control. In conclusion, dietary supplementation of mixture of probiotics and colistin improved quality of egg albumen, and reduced the egg cholesterol contents. They also reduced intestinal coli forms without harmful effects on overall productive and physiological responses in laying hens.

본 연구는 산란계에 Lactobacillus plantarum, Bacillus subtillis와 Aspergillus oryzae 및 colistin의 복합 첨가제를 급여하였을 때 난 생산성과 난질 및 난각질에 미치는 영향과 장내 미생물 조성, 계분 암모니아 발생량 및 난황 내 콜레스테롤 함량에 미치는 영향을 함께 조사하였다. 50주령의 Hy-Line Brown 산란계 160수를 공시하여 일반 사료(Control) 또는 0.2% 복합균종 생균제를 함유하는 실험 사료(T1, Bacillus subtilis + Aspergillus oryzae + Lactobacillus plantarum; T2, Bacillus subtilis + Aspergillus oryzae; T3, Bacillus subtilis + Aspergillus oryzae + colistin)를 8주간 급여하였다. 사료 섭취량, 난 생산성 및 간의 중량 그리고 난각 강도, 난각 두께, 난각색은 처리구 간 유의성 있는 차이가 관찰되지 아니하였다. 그러나 Haugh unit는 처리구가 Control에 비해 유의하게 높았다(P<0.05). 혈중 총 콜레스테롤 농도와 HDL 콜레스테롤은 Control에 비해 모든 처리구에서 감소하는 경향이 관찰되었으나 통계적 유의차는 없었다. 맹장 내 암모니아 농도에 미치는 영향은 Control에 비해 T1 처리구와 T3 처리구에서 유의하게 감소하는 것으로 나타났다(P<0.05). 총 균수와 lactic acid bacteria는 맹장 및 분내 모두 Control과 처리구간 큰 차이가 없었고, 맹장 및 분내의 coli forms은 Control에 비해 복합 생균제 처리구가 유의하게 낮거나(P<0.05) 낮은 경향이 관찰되었다. 4 주차와 8주차의 난황 콜레스테롤 농도는 복합 생균제 처리구가 Control에 비해 유의하게 감소하거나 감소하는 경향이 나타났다(P<0.05). 난황 중성지질과 인지질의 농도는 Control과 처리구간의 차이는 없는 것으로 나타났다. 결론적으로 본 실험을 통해서 생균제인 Lactobacillus plantarum, Bacillus subtillis, Aspergillus oryzae 그리고 항생제인 colistin으로 이루어진 복합제의 급여는 생산성과 혈액 성상에 부정적인 영향이 없이 난황 콜레스테롤과 분내 암모니아 농도를 감소시키고, 장내 coli forms를 저하시키는 결과가 관찰되었다. Colistin이라는 항생제는 생균제의 활성에 부정적인 영향 없이 생균제로만 이루어진 첨가제와 같은 효과를 내었으며, 장내 미생물 균총 조절에는 보다 효과적으로 작용하는 것으로 나타났다. 따라서 복합 생균제를 급여함으로 장내 균총과 산란계 사양에 유익한 효과를 기대할 수 있으며, colistin의 병용도 생균제의 이점을 저해하지 않는 것으로 사료된다.

Keywords

References

  1. Abdulrahim SM, Haddadin MSY, Hashlamoun EAR, Robinson RK 1996 The influence of Lactobacillus acidophilus and Bacitracin on layer performance of chickens and cholesterol content of plasma and egg yolk. Br Poult Sci 37:341-346 https://doi.org/10.1080/00071669608417865
  2. Catchpole CR, Andrews JW, Brenwald N, Wise R 1997 Areassessment of the in-vitro activity of colistin sulphomethate sodium. J Antimicrob Chemother 39:255-260 https://doi.org/10.1093/jac/39.2.255
  3. Chen YC, Chen TC 2003 Effects of commercial probiotic or prebiotic supplementation on production, size and quality of hens egg. Poult Sci 82:330
  4. Diaz GJ, Roldan LP, Cortes A 2003 Intoxication of Crotalria pallida seeds to growing broiler chick. Vet Hum Toxicol 45:187-189
  5. Duncan DB 1955 Multiple range and multiple F test. Biometric 11:1-4 https://doi.org/10.2307/3001478
  6. Gill HS, Guarner F 2004 Probiotics and human health: A clinical perspective. Postgraduate Med J 80:516-526 https://doi.org/10.1136/pgmj.2003.008664
  7. Grunewald KK, Mitchell K 1983 Serum cholesterol levels in mice fed fermented and unfermented acidophilus milk. J Food Protect 46:315-318 https://doi.org/10.4315/0362-028X-46.4.315
  8. Han SW, Lee KW, Lee BD, Sung CG 1999 Effect of feeding Aspergillus oryzae culture on fecal microflora, egg qualities, and nutrient metabolizabilities in laying hens. Asian-Aus J Anim Sci 12:417-421 https://doi.org/10.5713/ajas.1999.417
  9. Han SW, Lee KW, Lee BD, Sung CG 1999 Effect of feeding Aspergillus oryzae culture on fecal microflora, egg qualities, and nutrient metabolizabilities in laying hens. Asian-Aus J Anim Sci 12:417-421 https://doi.org/10.5713/ajas.1999.417
  10. Jin LZ, Ho YW, Abdullah N, Ali MA, Jalaudin S 1998 Effects of adherent Lactobacillus cultures on growth, weight of organs and intestinal microflora and volatile fatty acids in broilers. Anim Feed Sci Technol 70:197-209 https://doi.org/10.1016/S0377-8401(97)00080-1
  11. Jin LZ, Ho YW, Abdullah N, Jalaludin S 1997 Probiotics in poultry: modes of action. World's Poult Sci J 53:351-368 https://doi.org/10.1079/WPS19970028
  12. Kurtoglu V, Kurtoglu F, Seker E, Coskun B, Balevi T, Polat ES 2004 Effect of probiotic supplementation on laying hen diets on yield perfornance and serum and egg yolk cholesterol. Food Addit Contam 21:817-823 https://doi.org/10.1080/02652030310001639530
  13. Lesson S, Major D 1990 As biotechnology gains momentum Canadian researchers study need for feed criteria. Feedstuffs 62:23-30
  14. Lumeij JT 1997 Avian Clinical Biochemistry. Pages 857-883 In: Clinical Biochemistry of Domestic Animals. Kanebo JJ, Harvey JW, Bruss ML 5th Ed. Academic Press
  15. Mahdavi AH, Rahmani HR, Pourreza J 2005 Effect of probiotic supplements on egg quality and laying hen's performance. Int J Poult Sci 4:488-492 https://doi.org/10.3923/ijps.2005.488.492
  16. Mee BJ 1984 The selective capacity of pig feed additives and growth promotants for coilform resistance in antimicrobials in agriculture. Woodbine M Ed. Butter Worths, London. 349-358
  17. Mohan B, Kadirvel R, Natarajan A, Bhaskaran M 1996 Effect of probiotic supple mentation on growth, nitrogen utilization and serum cholesterol in broilers. Br Poult Sci 37:395-401 https://doi.org/10.1080/00071669608417870
  18. Mohan B, Kadirvel R, Bhaskaran M, Natarajan A 1995 Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. Br Poult Sci 36:799-803 https://doi.org/10.1080/00071669508417824
  19. Nahashon SN, Nakaue HS, Mirosh LW 1996 Performance of Comb White Leghorn layers fed a diet with a live microbial during the growth and egg laying phases. Anim Feed Sci Technol 57:25-38 https://doi.org/10.1016/0377-8401(95)00852-7
  20. Nahashon SN, Nakaue HS, Mirosh LW 1994 Production variables and nutrient retention in single comb white Leghorn laying pullets fed diets supplemented with directfed microbials. Poult Sci 73:1699-1711 https://doi.org/10.3382/ps.0731699
  21. Nelson CR, Gilliland SE 1984 Cholesterol uptake by Lactobacillus acidophilus. J Dairy Sci 67:50
  22. NRC 1994 Nutrient requirements of poultry. 9th ed. National Academy Press. Washington D.C
  23. Panda AK, Reddy MR, Rama Rao SV, Praharaj NK 2003 Production performance, serum/yolk cholesterol and immune competence of white Leghorn layer as influenced by dietary supplementation. Trop Anim Health Prod 35:85-94 https://doi.org/10.1023/A:1022036023325
  24. Patterson JA, Burkholder KM 2003 Application of prebiotics and probiotics in poultry production. Poult Sci 82:627-631 https://doi.org/10.1093/ps/82.4.627
  25. Roudaut B 1989 Depletion of colistin in eggs fllowing medication of laying hens. Vet 11:183-185 https://doi.org/10.1080/01652176.1989.9694219
  26. Rovinson RK 1997 Yogurt and health. Br Nutr Foundation Bull 21:191-194
  27. Rowghani E, Akbarian A 2007 Effects of a probiotic and other feed additives on performance and immune response of broiler chicks. Int J Poult Sci 6:261-265 https://doi.org/10.3923/ijps.2007.261.265
  28. Rowghani E, Akbarian A 2007 Effects of a probiotic and other feed additives on performance and immune response of broiler chicks. Int J Poult Sci 51:399-404
  29. Santoso U, Ohtani S, Tanaka K, Sakaida M 1999 Dried Bacillus subtilis culture reduced ammonia gas release in poultry house. AAAP and Korean Society of Anim Nut 12:806-809
  30. SAS 2002 SAS User's guide. Statistics, Version 8. e., SAS Institute Inc., Cary, NC
  31. Timmerman HM, Koning CJ, Mulder L, Rombouts FM, Beynen AC 2004 Monostrain, multistrain and multispecies probiotics : A comparison of functionality and efficacy. Int J Food Microbiol 15;96:219-33 https://doi.org/10.1016/0168-1605(92)90052-5
  32. Tortuero F, Fernandez E 1995 Effect s of inclusion of microbial cultures barley - based diet s fed to laying hens. Anim Feed Sci T echnol 53:255-265 https://doi.org/10.1016/0377-8401(94)00747-W
  33. Tortuero F 1973 Influence of implantation of Lactobacillus acidophilus in chicks on the growth, feed conversion, malabsorption of fats syndrome and intestinal flora. Poult Sci 52: 197.203
  34. van Hattum CP, De Kleyne J, Grintjes G 2000 Efficacy of amoxicillin/colistin in gastro-intestinal E. coli infections complicated with Streptococcus suis in weaned piglets. In: Proceedings of the 8th EAVPT Congress, Jerusalem
  35. Xu CL, Ji C, Ma Q, Hao K, Jin ZY, Li K 2006 Effects of a dried Bacillus subtilis culture on egg quality. Poult Sci 85: 364-368 https://doi.org/10.1093/ps/85.2.364
  36. Yeo J, Kim K 1997 Effect of feeding diets containing an antibiotic, a probiotic, or Yucca extract on growth and intestinal urease activity in broiler chicks. Poult Sci 76:381.385
  37. Ziv G 1981 Clonocal pharmacology of polymyxins. J Amer Vet Med Assosiation 179:711-715
  38. 김상호 유동조 박수영 이상진 최철환 나재천 류경선 2002 산 란계의 생산성, 영양소 소화율 분의 암모니아 발생량 및 장내 미생물 변화에 대한 유산균의 급여 효과. 한국가금학회지 29:213-223
  39. 김용란 안병기 김문수 강창원 2000 생균제(MS102)의 사료 내 첨가가 육계 성적과 혈중 콜레스테롤, 소장 크기 및 장내 균총에 미치는 영향. 동물자원과학회지 42:849-858
  40. 김해진 유종상 권오석 민병준 손경승 조진호 진영걸 김인호 2005 산란계에 Bacillus subtillis의 급여가 계란 품질, 혈액 성상 및 분내 암모니아태 질소 함량에 미치는 영향. 한국가금학회지 32:9-14
  41. 유선종 2007 사료 내 천연물질과 필수미량광물질 첨가에 의 한 저콜레스테롤 계란의 생산. 건국대학교 대학원 박사 학위논문