DOI QR코드

DOI QR Code

감마선 조사에 의한 분유 단백질의 항원성 저감화

Reduction of the Antigenicity of Powdered Milk by Gamma Irradiation

  • 발행 : 2008.08.30

초록

본 연구는 안전성 확보차원에서 식품분야에 활용성이 증가하고 있는 감마선 조사처리가 유제품의 항원성 저감에도 효과가 있음을 보이기 위해 수행되었다. 모세관전기영동 결과 ${\alpha}_{S1}$-케이신과 ${\beta}_{A1}$-케이신이 ${\alpha}_{S0}$-케이신과 ${\beta}_{A2}$-케이신보다 상대적으로 많이 분해되었음을 확인하였다. cELISA를 이용한 in vitro 시험 결과와 기니픽을 이용한 수동피부아나필랙시스(Passive cutaneous anaphylaxis) 반응을 통해 10 kGy의 감마선 조사에서 뚜렷한 항원성 저감을 확인하였다. 이상의 결과는 감마선 조사가 우유 단백질의 항원, 특히 ${\alpha}_{S1}$-케이신과 ${\beta}_{A1}$-케이신의 구조를 변화시켜 항원성을 감소시키는 것으로 판단되며, 따라서 감마선 조사는 알레르기 유발 식품의 항원성 감소에 유용한 기술로 생각된다.

The aim of this work was to examine the reduction in antigenicity of milk proteins in powdered milk by gamma irradiation which is increasingly used for food safety. Skim milk powder samples were exposed to irradiation doses of 1, 5, and 10 kGy. A greater reduction of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein was found than ${\alpha}_{S0}$-casein and ${\beta}_{A2}$-casein by capillary electrophoresis. Competitive indirect ELISA and passive cutaneous anaphylaxis tests using guinea pigs showed a reduction in antigenicity of powdered milk by 10kGy gamma irradiation. These results indicated that gamma irradiation reduce allergenicity of milk proteins by structural changes of ${\alpha}_{S1}$-casein and ${\beta}_{A1}$-casein, and can be useful for dairy products.

키워드

참고문헌

  1. AOAC (1995) Official Methods of Analysis, 16th ed., Association of Official Analytical Chemists, Washington, DC, USA
  2. Byun, M. W., Kim, J. H., Lee, J. W., Park, J. W., Jong, C. S., and Kang, I. J. (2000) Effects of gamma irradiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. J. Food Prot. 63, 940-944 https://doi.org/10.4315/0362-028X-63.7.940
  3. Cho, K. H., Yook, H. S., Lee, J. W., Lee, S. Y., and Byun, M. W. (2001) Changes of binding ability of milk-hypersensitive patients' IgE to gamma-irradiated milk proteins. J. Korean Soc. Food. Sci. Nutr. 30, 505-509
  4. Choi, S. H., Lee, S. B., and Choi, J. W. (2005) Microbiological safety of infant formula and baby food. Korean J. Dairy Sci. Technol. 23, 65-71
  5. Ciesla, K., Salmieri, S., Lacroix, M., and Le Tien, C. (2004) Gamma irradiation influence on physical properties of milk proteins. Radiat. Phys. Chem. 71, 93-97
  6. De Jong, N., Visser, S., and Olieman, C. (1993) Determination of milk proteins by capillary electrophoresis. J. Chromatography A. 652, 207-213 https://doi.org/10.1016/0021-9673(93)80661-Q
  7. Dong, F. M., Hashisaka, A. E., Rasco, B. A., Einstein, M. A., Mar, D. R. and Aker, S. N. (1992) Irradiated or aseptically prepared frozen dairy desserts: acceptability to bone marrow transplant recipients. J. Am. Diet Assoc. 92, 719-723
  8. Elsayed, S., Hill, D. J., and Do, T. V. (2004) Evaluation of the allergenicity and antigenicity of bovine-milk $\alpha_{s1}$- casein using extensively purified synthetic peptides. Scand. J. Immunol. 60, 486-493 https://doi.org/10.1111/j.0300-9475.2004.01493.x
  9. Filali-Mouhim, A., Audette, M., St-Louis, M., Thauvette, L., Denoroy, L., Penin, F., Chen, X., Rouleau, N., Le Caer, J. P., Rossier, J., Potier, M., and Le Maire, M. (1997) Lysozyme fragmentation induced by gamma-radiolysis. Int. J. Radiat. Biol. 72, 63-70 https://doi.org/10.1080/095530097143545
  10. Fenaille, F., Parisod, V., Tabet, J. C., and Guy, P. A. (2005) Carbonylation of milk powder proteins as a consequence of processing conditions. Proteomics 5, 3097-3104 https://doi.org/10.1002/pmic.200401139
  11. Ham, J. S., Noh, Y. B., Kim, S. I, Kim, H. S., Jeong, S. G., Chae, H. S., Ahn, J. N., Jo, C., and Lee, Y. K. (2005) Changes of chemical, bacteriological, and allergenicity of raw milk by gamma irradiation. Korean J. Dairy Sci. Technol. 23, 93-98
  12. Ham, J. S., Jeong, S. G., Noh, Y. B., Shin, J. H., Han, G. S., Chae, H. S., Yoo, Y. M., Ahn, J. N., Lee, J. W., Jo, C., and Lee, Y. K. (2007) Effects of gamma irradiation on Queso Blanco cheese. Korean J. Dairy Sci. Technol. 25, 15-20
  13. Ham, J. S., Shin, J. H., Noh, Y. B., Jeong, S. G., Han, G. S., Chae, H. S., Yoo, Y. M., Ahn, J. N., Lee, Y. K., and Jo, C. (2008) Chemical and microbiological quality, capillary electrophoresis pattern, and rennet coagulation of UHT-treated and irradiated milk. Food Sci. Biotechnol. 17, 58-65
  14. Horowits, R., Kempner, E. S., Bisher, M. E., and Podolsky, R. J. (1986) A physiological role for titin and nebulin in skeletal muscle. Nature 323, 160-164 https://doi.org/10.1038/323160a0
  15. Kim, D. W., Choe, H. S., Ahn, C. N., Jeong, S. G., Ham, J. S., and In, Y. M. (2003) Reduction of antigenicity of bovine casein by microbial enzymes. Korean J. Dairy Sci. Technol. 21, 97-104
  16. Lee, J. W., Yook, H. S., Lee, K. H., Kim, J. H., and Byun, M. W. (2000) Conformational changes of myosin by gamma irradiation. Radiat. Phys. Chem. 58, 271-277 https://doi.org/10.1016/S0969-806X(99)00466-1
  17. Lee, J. W., Kim, J. H., Yook, H. S., Kang, K. O., Lee, S. Y., Hwang, H. J., and Byun, M. W. (2001) Effects of gamma radiation on the allergenic and antigenic properties of milk proteins. J. Food Prot. 64, 272-276 https://doi.org/10.4315/0362-028X-64.2.272
  18. Lee, J. W., Oh, S. H., Kim, J. H., Yook, H. S., and Byun, M. W. (2006) Gamma radiation sensitivity of Enterobacter sakazakii in dehydrated powdered infant formula. J. Food Prot. 69, 1434-1437 https://doi.org/10.4315/0362-028X-69.6.1434
  19. Revilla, I, Vivar-Quintana, A. M., and Rodriguez-Nogales, J. M. (2005) Evaluation of the effect of somatic cell counts on casein proteolysis in ovine milk cheese by means of capillary electrophoresis. J. Capill. Electrophor. Microchip Technol. 9, 45-52
  20. Rowe, B., Begg, N. T., Hutchinson, D. N., Dawkin, H. C., Gilbert, R. J., Jacob, M., Hales, B. H., Rae, F. A., and Jepson, M. (1987) Salmonella ealing infections associated with consumption of infant dried milk. Lancet 2, 900-903
  21. Van Acker, J., de Smet, F., Muyldemans, G., Anne Naessens, A., and Lauwers, S. (2001) Outbreak of necrotizing enterocolitis associated with Enterobacter sakazakii in powdered milk formula. J. Clin. Microbiol. 39, 293-297 https://doi.org/10.1128/JCM.39.1.293-297.2001

피인용 문헌

  1. Effects of Autoclave and Microwave Treatments on the Antigenicity of Gliadin in Medium Wheat Dough vol.44, pp.1, 2012, https://doi.org/10.9721/KJFST.2012.44.1.055
  2. Changes in Antigenicity of Gliadin from Medium Flour by Autoclave and Microwave Treatments vol.40, pp.10, 2011, https://doi.org/10.3746/jkfn.2011.40.10.1423
  3. Recent advances in the application of capillary electromigration methods for food analysis and Foodomics vol.31, pp.1, 2010, https://doi.org/10.1002/elps.200900365