Food Science and Biotechnology

  • 제17권3호
  • /
  • Pages.623-630
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  • 2008
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  • 1226-7708(pISSN)
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  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Determination of Aflatoxin B1 in Rice, Barley, and Feed by Non-instrumental Immunochromatographic Strip-test and High Sensitive ELISA

  • Shim, Won-Bo (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Kim, Jung-Sook (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Kim, Ji-Young (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Choi, Jin-Gil (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Je, Jung-Hyun (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University) ;
  • Kuzmina, Nina Sergeevna (Mechnikov Research Institute of Vaccine and Sera of Russian Academy of Medical Sciences) ;
  • Eremin, Sergei Alexandrovich (Division of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University) ;
  • Chung, Duck-Hwa (Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University)
  • 발행 : 2008.06.30
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초록

A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DC-ELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both methods were coincided with the amount spiked AFB1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the ICG strip-test is easier to perform and quicker, but less sensitivity than DC-ELISA. Both methods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the ICG strip-test can be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

키워드

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