Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Polysaccharides from Edible Mushroom Hinmogi (Tremella fuciformis) Inhibit Differentiation of 3T3-L1 Adipocytes by Reducing mRNA Expression of $PPAR{\gamma}$, C/$EBP{\alpha}$, and Leptin

  • Jeong, Hye-Jin (Department of Biotechnology, Yonsei University) ;
  • Yoon, Seon-Joo (Graduate Program in Biomaterials Science and Engineering, Yonsei University) ;
  • Pyun, Yu-Ryang (Department of Biotechnology, Yonsei University)
  • Published : 2008.04.30
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Abstract

Water-soluble fraction (WSF) from edible mushroom hinmogi (Tremella fuciformis) were obtained by water extraction, and polysaccharides in the WSF were separated by ethanol precipitation. The inhibitory effects of the polysaccharides on 3T3-L1 adipocyte differentiation were evaluated by the reduction of peroxisome proliferators-activated receptor ${\gamma}$ ($PPAR{\gamma}$) translation, triglyceride accumulation, Oil Red-O staining, and expression levels of $PPAR{\gamma}$, CCAAT/enhancer binding protein a (C/$EBP{\alpha}$), and leptin. The $PPAR{\gamma}$ translation in 3T3-L1 cells was inhibited by the treatment with polysaccharide precipitated by 80% ethanol (P80) which showed highest inhibitory activity among polysaccharides tested. In addition, treatment of P80 to 3T3-L1 cells significantly inhibited the triglyceride accumulation, Oil Red-O staining, and mRNA expression of $PPAR{\gamma}$, C/$EBP{\alpha}$, and leptin in a dose-dependent manner. Based upon these results, P80 from edible mushroom hinmogi shows the inhibitory activity on the differentiation of 3T3-L1 adipocytes. Therefore, it might be employed as a potential anti-obesity material.

Keywords

References

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