Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of DHU001, a Mixed Herbal Formula on Acute Inflammation in Mice

  • Back, Young-Doo (Department of Herbal Biotechnology, College of Oriental Medicine, Daegu Haany University) ;
  • Lee, Hyeung-Sik (Department of Herbal Biotechnology, College of Oriental Medicine, Daegu Haany University) ;
  • Ku, Sae-Kwang (Department Anatomy and Histology, College of Oriental Medicine, Daegu Haany University)
  • Published : 2008.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effects of DHU001, a mixed herbal formula consisted of 7 types aqueous extracts for treating respiratory disorders were observed on xylene-induced acute inflammation. The xylene was topically applied 60 min after administration of 500, 250 and 125 mg/kg of DHU001, and all animals were sacrificed 2 hrs after xylene application. The changes on ear weights, histolopathological analyses of ear were evaluated and compared to those of indomethacin and dexamethasone(15 mg/kg treated)-Both of drugs are well-known by anti-inflammatory agents. Xylene application resulted in marked increases in induced ear weights as compared with intact control ear. Severe vasodilation, edematous changes of ear skin and increase in the thickness of the ear tissues, neutrophil infiltration as acute inflammation were detected in xylene-treated control ears at histopathological observation. However, these xylene-induced acute inflammatory changes were dose-dependently decreased by oral treatment of DHU001. Therefore, it is concluded that DHU001 has favorable anti-inflammatory effects on xylene-applicated acute ear inflamed mice.

Keywords

References

  1. Ahn, K.S., Noh, E.J., Zhao, H.L., Jung, S.H., Kang, S.S. and Kim, Y.S. (2005). Inhibition of inducible nitric oxide synthase and cyclooxygenase II by Platycodon grandiflorum saponins via suppression of nuclear factor-kappaB activation in RAW 264.7 cells. Life Sci., 76, 2315-2328 https://doi.org/10.1016/j.lfs.2004.10.042
  2. Aimbire, F., Penna, S.C., Rodrigues, M., Rodrigues, K.C., Lopes-Martins, R.A. and Sertié, J.A. (2007). Effect of hydroalcoholic extract of Zingiber officinalis rhizomes on LPS-induced rat airway hyperreactivity and lung inflammation. Prostaglandins Leukot Essent Fatty Acids, 77, 129-138 https://doi.org/10.1016/j.plefa.2007.08.008
  3. Al-Majed, A.A., Khattab, M., Raza, M., Al-Shabanah, O.A. and Mostafa, A.M. (2003). Potentiation of diclofenacinduced anti-inflammatory response by aminoguanidine in carrageenan- induced acute inflammation in rats: the role of nitric oxide. Inflamm. Res., 52, 378-382 https://doi.org/10.1007/s00011-003-1189-1
  4. Amin, A.R., Vyas, P., Attur, M., Leszczynska-Piziak, J., Patel, I.R., Weissmann, G. and Abramson, S.B. (1995). The mode of action of aspirin-like drugs: effect on inducible nitric oxide synthase. Proc. Natl. Acad. Sci. USA, 92, 7926-7930
  5. Baliga, M.S., Jagetia, G.C., Rao, S.K. and Babu, K. (2003). Evaluation of nitric oxide scavenging activity of certain spices in vitro: a preliminary study. Nahrung, 47, 261-264 https://doi.org/10.1002/food.200390061
  6. Ghayur, M.N., Gilani, A.H. and Janssen, L.J. (2008). Ginger attenuates acetylcholine-induced contraction and $Ca^{2+}$ signalling in murine airway smooth muscle cells. Can. J. Physiol. Pharmacol., 86, 264-271 https://doi.org/10.1139/Y08-030
  7. Habashy, R.R., Abdel-Naim, A.B., Khalifa, A.E. and Al-Azizi, M.M. (2005). Anti-inflammatory effects of jojoba liquid wax in experimental models. Pharmacol. Res., 51, 95-105 https://doi.org/10.1016/j.phrs.2004.04.011
  8. Han, B.H., Yoo, S.Y., Park M.H. and Lee, H.J. (1981). Antioxidant activity screening on crude drugs. J. Pharmacogn., 12, 66
  9. Hosseinzadeh, H. and Younesi, H.M. (2002). Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol., 2, 7-12 https://doi.org/10.1186/1471-2210-2-7
  10. Hosseinzadeh, H., Haddadkhodaparast, M.H. and Arash, A.R. (2003). Antinociceptive, antiinflammatory and acute toxicity effects of Salvia leriifolia Benth seed extract in mice and rats. Phytother. Res., 17, 422-425 https://doi.org/10.1002/ptr.1154
  11. Jang, M.H., Kim, C.J., Kim, E.H., Kim, M.G., Leem, K.H. and Kim, J. (2006). Effects of Platycodon grandiflorum on lipopolysaccharide-stimulated production of prostaglandin E2, nitric oxide, and interleukin-8 in mouse microglial BV2 cells. J. Med. Food, 9, 169-174 https://doi.org/10.1089/jmf.2006.9.169
  12. Kim, H.D., Cho, H.R., Moon, S.B., Shin, H.D., Yang, K.J., Park, B.R., Jang, H.J., Kim, L.S., Lee, H.S. and Ku, S.K. (2007). Effects of beta-glucan from Aureobasidium pullulans on acute inflammation in mice. Arch. Pharm. Res., 30, 323-328 https://doi.org/10.1007/BF02977613
  13. Kim, J.Y., Hwang, Y.P., Kim, D.H., Han, E.H., Chung, Y.C., Roh, S.H. and Jeong, H.G. (2006). Inhibitory effect of the saponins derived from roots of Platycodon grandiflorum on carrageenan-induced inflammation. Biosci. Biotechnol. Biochem., 70, 858-864 https://doi.org/10.1271/bbb.70.858
  14. Kim, S.S., Lee, S.C., Shin, H.D., Shin, M.K., Kim J.H. and Song, H.J. (2004). Studies on allergy asthma effect of Radix Platicodi. Kor. J. Herbology, 19, 61-70
  15. Ko, K.M. and Lam, B.Y. (2002). Schisandrin B protects against tert-butylhydroperoxide induced cerebral toxicity by enhancing glutathione antioxidant status in mouse brain. Mol. Cell Biochem., 238, 181-186 https://doi.org/10.1023/A:1019907316129
  16. Kou, J., Ma, R., Zhu, D. and Yan, Y. (2003). Blood-activating and anti-inflammatory actions of Polygala fallax. Zhong Yao Cai, 26, 268-271
  17. Kou, J., Ni, Y., Li, N., Wang, J., Liu, L. and Jiang, Z.H. (2005). Analgesic and anti-inflammatory activities of total extract and individual fractions of Chinese medicinal ants Polyrhachis lamellidens. Biol. Pharm. Bull., 28, 176-180 https://doi.org/10.1248/bpb.28.176
  18. Lee, K.J., Choi, C.Y., Chung, Y.C., Kim, Y.S., Ryu, S.Y., Roh, S.H. and Jeong, H.G. (2004). Protective effect of saponins derived from roots of Platycodon grandiflorum on tert-butyl hydroperoxide-induced oxidative hepatotoxicity. Toxicol. Lett., 147, 271-282 https://doi.org/10.1016/j.toxlet.2003.12.002
  19. Masuda, Y., Kikuzaki, H., Hisamoto, M. and Nakatani, N. (2004): Antioxidant properties of gingerol related compounds from ginger. Biofactors, 21, 293-296 https://doi.org/10.1002/biof.552210157
  20. Minghetti, P., Sosa, S., Cilurzo, F., Casiraghi, A., Alberti, E., Tubaro, A., Loggia, R.D. and Montanari, L. (2007). Evaluation of the topical anti-inflammatory activity of ginger dry extracts from solutions and plasters. Planta Medica, 73, 1525-1530 https://doi.org/10.1055/s-2007-993741
  21. Narimanian, M., Badalyan, M., Panosyan, V., Gabrielyan, E., Panossian, A., Wikman, G. and Wagner, H. (2005). Impact of Chisan (ADAPT-232) on the quality-of-life and its efficacy as an adjuvant in the treatment of acute nonspecific pneumonia. Phytomedicine, 12, 723-729 https://doi.org/10.1016/j.phymed.2004.11.004
  22. Ojewole, J.A. (2005). Antinociceptive, anti-inflammatory and antidiabetic effects of Bryophyllum pinnatum (Crassulaceae) leaf aqueous extract. J. Ethnopharmacol., 99, 13-19 https://doi.org/10.1016/j.jep.2005.01.025
  23. Ojewole, J.A. (2006). Analgesic, antiinflammatory and hypoglycaemic effects of ethanol extract of Zingiber officinale (Roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytother. Res., 20, 764-772 https://doi.org/10.1002/ptr.1952
  24. Park, E., Kum, S., Wang, C., Park, S.Y., Kim, B.S. and Schuller-Levis, G. (2005). Anti-inflammatory activity of herbal medicines: inhibition of nitric oxide production and tumor necrosis factor-alpha secretion in an activated macrophage- like cell line. Am. J. Chin. Med., 33, 415-424 https://doi.org/10.1142/S0192415X05003028
  25. Park, J.H. and Geon, D.G. (2003). Pharmacognostical studies on the Chinese crude drug "Maig Moon Dong". Kor. J. Pharmacogn., 34, 6-9
  26. Perez, C., Canal, J.R. and Torres, M.D. (2003). Experimental diabetes treated with Ficus carica extract: effect on oxidative stress parameters. Acta Diabetol., 40, 3-8 https://doi.org/10.1007/s005920300001
  27. Ramprasath, V.R., Shanthi, P. and Sachdanandam, P. (2006). Immunomodulatory and anti-inflammatory effects of Semecarpus anacardium LINN. Nut milk extract in experimental inflammatory conditions. Biol. Pharm. Bull., 29, 693-700 https://doi.org/10.1248/bpb.29.693
  28. Rhyu, M.R., Kim, E.Y., Yoon, B.K., Lee, Y.J. and Chen, S.N. (2006). Aqueous extract of Schizandra chinensis fruit causes endothelium-dependent and -independent relaxation of isolated rat thoracic aorta. Phytomedicine, 13, 651-657 https://doi.org/10.1016/j.phymed.2006.02.003
  29. Shin, T.Y. (2003). Inhibition of immunologic and nonimmunologic stimulation-mediated anaphylactic reactions by the aqueous extract of Mentha arvensis. Immunopharmacol. Immunotoxicol., 25, 273-283 https://doi.org/10.1081/IPH-120020475
  30. Sun, H.X. and Pan, H.J. (2006). Immunological adjuvant effect of Glycyrrhiza uralensis saponins on the immune responses to ovalbumin in mice. Vaccine, 24, 1914-1920 https://doi.org/10.1016/j.vaccine.2005.10.040
  31. Tan, B.K. and Vanitha, J. (2004). Immunomodulatory and antimicrobial effects of some traditional chinese medicinal herbs: a review. Curr. Med. Chem., 11, 1423-1430 https://doi.org/10.2174/0929867043365161
  32. Wang, C., Schuller Levis, G.B., Lee, E.B., Levis, W.R., Lee, D.W., Kim, B.S., Park, S.Y. and Park, E. (2004). Platycodin D and D3 isolated from the root of Platycodon grandiflorum modulate the production of nitric oxide and secretion of TNF-alpha in activated RAW 264.7 cells. Int. Immunopharmacol., 4, 1039-1049 https://doi.org/10.1016/j.intimp.2004.04.005
  33. Yoon, Y.D., Han, S.B., Kang, J.S., Lee, C.W., Park, S.K., Lee, H.S., Kang, J.S. and Kim, H.M. (2003). Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum. Int. Immunopharmacol., 3, 1873-1882 https://doi.org/10.1016/j.intimp.2003.09.005
  34. Zhou, Y. and Xu, R. (1992). Antioxidative effect of Chinese drugs. Zhongguo Zhong Yao Za Zhi, 17, 368-369

Cited by

  1. Effect of DHU001, a Polyherbal Formula on Formalin-induced Paw Chronic Inflammation of Mice vol.27, pp.2, 2011, https://doi.org/10.5487/TR.2011.27.2.095