Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
권호 표지
DOI QR코드

DOI QR Code

Callus Induction and Plant Regeneration from Mature Seeds of Timothy

티모시 성숙종자로부터 캘러스 유도 및 식물체 재분화

  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Timothy (Phleum pratense L.) is an important grass species as forage. In order to optimize tissue culture conditions of timothy, the effects of plant growth regulators on callus induction and plant regeneration was investigated with mature seeds of colt cultivar. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 3 mg/L. The highest embryogenic callus frequenc (25%) was observed when the mature seed were cultured on MS medium supplemented with 3 mg/L 2,4-D and 0.1 mg/L BA. The highest plant regeneration frequency was observed when type B callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of timothy by the production of transgenic plant.

티모시의 colt 품종을 이용하여 최적 조직배양조건을 확립하기 위하여 성숙종자를 배양함에 있어 첨가되어지는 식물 생장조절 물질의종류와 농도가 배발생 캘러스 유도 및 식물체재분화에 미치는 영향을 조사하였다. 배발생 캘러스 유도시 첨가되는 2,4-D는 3 mg/L 첨가 구에서 78%로 캘러스 유도율이 가장 높았으며, 3 mg/L 2,4-D와 0.1 mg/L의 BA를 첨가하여 배양했을 때 형성된 캘러스는 조직적으로 치밀하며 유백색의 배발생 캘러스가 가장 많이 형성되었다. Type B 캘러스만을 이용하여 식물체재분화에는 1 mg/L 2,4-D와 3 mg/L BA가 첨가된 배지에 캘러스를 배양했을 때 25.3%의 재분화율을 나타내었다. 본 연구를 통하여 확립된 효율적인 재분화 시스템은 분자육종을 통한 신품종 티모시의 개발에 유용하게 이용될 수 있을 것이다.

Keywords

References

  1. Bettany, A.J.E., S.J. Dalton, E. Timms, B. Manderyck, M.S. Dhanoa and P. Morris. 2003. Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.). Plant Cell Rep. 21:437-444 https://doi.org/10.1007/s00299-002-0531-3
  2. Bregitzer P. 1992. Plant regeneration and callus in barley: effects of geno and culture medium. Crop Sci. 32:1108-1112 https://doi.org/10.2135/cropsci1992.0011183X003200050007x
  3. Carvalho, C.H.S., N. Bohorova, P.N. Bordallo, L. L. Abreu, F.H. Valicente, W. Bressan and E. Paiva. 1997. Type II callus production and plant regeneration in tropical maize genotypes. Plant Cell Rep. 17:73-76 https://doi.org/10.1007/s002990050355
  4. Chai, B.F., A.H. Liang, W. Wang and W. Hu. 2003. Agrobacterium-mediated transformation of kentucky bluegrass. Acta Bot. Sinica. 45: 966-973
  5. Chaudhury, A. and Q. Rongda. 2000. Somatic embryogenesis and plant regeneration of turf- Bermuda grass: effect of 6-benzyladenine in callus induction medium. Plant Cell. Tiss Org. Cult. 60: 113-120 https://doi.org/10.1023/A:1006456005961
  6. Chen, L., C.K. Auh, P. Dowling, J. Bell, F. Chen, A. Hopkins, R. A. Dixon and Z.Y. Wang. 2003. Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down- regulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol. J. 1:437-449 https://doi.org/10.1046/j.1467-7652.2003.00040.x
  7. Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668
  8. Dong, S. and R. Qu. 2005. High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Science 168:1453-1458 https://doi.org/10.1016/j.plantsci.2005.01.008
  9. Griffin, J.D. and M.S. Dibble. 1995. High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.). Plant Cell Report. 14:721-724
  10. Guo, Y.D. and S. Pulli. 2001. Somatic embryogenesis and plant regeneration from suspension cultures of timothy (Phleum pratense L.). Act Agr Scand B-S P. 51: 160-166
  11. Hisano, H, A. Kanazawa, A. Kawakami, M. Yoshida, Y. Shimamoto and T. Yamada. 2004. Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. Plant Science. 167:861-868 https://doi.org/10.1016/j.plantsci.2004.05.037
  12. Lee, S.-H., D.-G. Lee, H.-S. Woo and B.-H. Lee. 2004. Development of transgenic tall fescue plants from mature seed-derived callus via Agrobacteriummediated transformation. Asian-Aust. J.Anim. Sci. 17:1390- 1394
  13. Lee, S.-H., D.-G. Lee, H.-S. Woo, K.-W. Lee, D.-H. Kim, S.-S. Kwak, J.-S. Kim, H. Kim, N. Ahsan, M.S. Choi, J.-K. Yang and B-H Lee. 2006. Production of transgenic orchardgrass via Agrobacterium-mediated transformation of seed- derived callus tissues. Plant Science, 171:408- 414 https://doi.org/10.1016/j.plantsci.2006.05.006
  14. Lee, S.-H., N. Ahsan, K.-W. Lee, D.-H. Kim, D.-G. Lee, S.-S. Kwak, S.-Y. Kwon, T.H. Kim and B.-H. Lee. 2007. Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol. 164:1626-1638 https://doi.org/10.1016/j.jplph.2007.01.003
  15. Lee, K.-W., S.-H. Lee, D.-H. Kim, D.-G. Lee, S.-H. Won, H. Lee and B.-H. Lee. 2006. Effect of callus and antioxidants on plant regeneration and transformation of tall fescue. Journal of the korean society of grassland science. 26:77-82 https://doi.org/10.5333/KGFS.2006.26.2.077
  16. Miki, K., T. Koichi, C.H, Bae and H.Y. Lee. 2003. Plant regeneration and transformation of kentucky bluegrass (Poa pratensis L.) via the plant tissue culture. Korea J. plant biotech. 30:115-121 https://doi.org/10.5010/JPB.2003.30.2.115
  17. Montague, A., A. Ziauddin, R. Lee, W.M. Ainley and J. Strommer. 2008. High-efficiency phos- phinothricin-based selection for alfalfa. trans- formation. Plant Cell Tissue Organ Culture. DOI 10.1007/s11240-007-9274-8
  18. Murashige, T. and Skoog, F. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  19. Plazek, A., M. Filek and M. Wfdzony. 1999. Improvement of regeneration ability in Phleum pratense L. in vitro culture by dicamba. Acta Physiologiae Plantarum. 21:397-403 https://doi.org/10.1007/s11738-999-0012-1
  20. Sato, H. and T. Takamizo. 2006. Agrobacterium tumefaciens-mediated transformation of forage- perennial ryegrass (Lolium perenne L.). Grassland Science. 52:95-98 https://doi.org/10.1111/j.1744-697X.2006.00053.x
  21. Weeks, J.T., J. Ye and C.M. Rommens. 2008. Development of an in planta method for transformation of alfalfa. Transgenic Research. 10.1007/s11248-007-9132-9
  22. Wu, Y.-Y., Q.-J. Chen, M. Chen, J. Chen and X.-C. Wang. 2005. Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na+/H+ antiporter gene. Plant Science. 169: 165-173 https://doi.org/10.1016/j.plantsci.2005.03.011
  23. Zhong, H. and M. B. Sticklen. 1991. Plant regeneration via somatic embryogenesis in creeping bentgrass. Plant Cell Report. 10:453- 456 https://doi.org/10.1007/BF00233813

Cited by

  1. Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius) vol.26, pp.2, 2013, https://doi.org/10.7732/kjpr.2013.26.2.320