YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Planting Conditions of Korean Cannabis Derived from Stable Isotope Ratio & Tetrahydrocannabinol Contents

안정동위원소 비율 및 테트라하이드로칸나비놀 함량 분석을 통한 한국산 대마의 재배 환경 추론

  • Lee, Jae-Sin (National Institute of Scientific Investigation, Department of Forensic Science) ;
  • Park, Yong-Hoon (National Institute of Scientific Investigation, Department of Forensic Science) ;
  • Rhee, Jong-Sook (National Institute of Scientific Investigation, Department of Forensic Science) ;
  • Jeong, Jin-Il (National Institute of Scientific Investigation, Department of Forensic Science) ;
  • Lim, Mi-Ae (National Institute of Scientific Investigation, Department of Forensic Science) ;
  • Chung, Hee-Sun (National Institute of Scientific Investigation, Department of Forensic Science)
  • 이재신 (국립과학수사연구소 법과학부) ;
  • 박용훈 (국립과학수사연구소 법과학부) ;
  • 이종숙 (국립과학수사연구소 법과학부) ;
  • 정진일 (국립과학수사연구소 법과학부) ;
  • 임미애 (국립과학수사연구소 법과학부) ;
  • 정희선 (국립과학수사연구소 법과학부)
  • Published : 2008.06.30
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Abstract

Stable isotope ratio of carbon and nitrogen ($\delta^{13}C$ & $\delta^{15}N$), and $\Delta^{9}$-tetrahydrocannabinol (THC) contents were measured on 37 Korean cannabis and 10 commercial grade marijuana seized in Korea. Factors influencing on the measured values and their variations were investigated. $\delta^{13}C$ value of cannabis is specified mainly by water availability. Korean cannabis showed relatively low $\delta^{13}C$ values ranging -33.29$\sim$-27.01% (mean=-31.01%), which reflect geographic conditions of Korea where is rainy, especially during summer. $\delta^{15}N$ values, which reflect individual planting conditions, were relatively high up to -0.5$\sim$18.0% (mean=6.44%). It reflects characteristics of Korean cannabis growing wild in forest or cultivated in fertile soil. Tetrahydrocannabinol is the major hallucinogenic compound of cannabis. Ethanol extracts of cannabis leaves were derivatized by N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), and the derivatives were analyzed by GC-MS in selected ion monitoring (SIM) mode. THC contents of Korean cannabis ranged 0.11$\sim$4.34% (mean=1.47%), which were relatively low compared with commercial grade marijuana.

Keywords

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