대한한방내과학회지 (The Journal of Internal Korean Medicine)
- 제29권2호
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- Pages.318-333
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- 2008
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- 1226-9174(pISSN)
청조구폐탕(淸燥救肺湯)과 이음전(理陰煎)이 호흡기 접액분비에 미치는 영향
Effects of Cheongjogupye-tang(淸燥救肺湯) and Yieum-jeon(理陰煎) on Secretion of Mucin from Respiratory Epithelial Cells
- Park, Wan-Yeol (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University) ;
- Seo, Un-Kyo (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dongguk University)
- 발행 : 2008.06.30
초록
Objectives : In this study, the author tried to examine whether Cheogjogupye-tang (淸燥救肺湯, CGPT) and Yieum-jeon (理陰煎, YEJ) significantly affect in vitro and in vivo mucin secretion, MUC5AC gene expression in airway epithelial cells and contractility of isolated tracheal smooth muscle of rabbit. Materials and Methods : For in vitro experiment, confluent hamster tracheal surface epithelial (HTSE) cells were chased for 30 minutes in the presence of CGPT and YEJ to assess the effects of the agents on mucin secretion by enzyme-linked immunosorbent assay (ELISA), with removal of oriental herbal medicine extract from each agent-treated sample by centrifuge microfilter. Also, the effects of the agents on TNF-alpha or EGF-induced MUC5AC gene expression in human airway epithelial cells (NCI-H292) were investigated. Possible cytotoxicities of the agent were assessed by examining both LDH release from HTSE cells and the rate of survival and proliferation of NCI-H292 cells. For in vivo experiment, hypersecretion of airway mucin and goblet cell hyperplasia was induced by exposure of rats to