Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Carbon and Energy Balances of Glucose Fermentation with Hydrogen-producing Bacterium Citrobacter amalonaticus Y19

  • Oh, You-Kwan (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Park, Sung-Hoon (Department of Chemical and Biochemical Engineering and Institute for Environmental Technology and Industry, Pusan National University) ;
  • Seol, Eun-Hee (Department of Chemical and Biochemical Engineering and Institute for Environmental Technology and Industry, Pusan National University) ;
  • Kim, Seo-Hyoung (Department of Chemical and Biochemical Engineering and Institute for Environmental Technology and Industry, Pusan National University) ;
  • Kim, Mi-Sun (Bioenergy Research Center, Korea Institute of Energy Research) ;
  • Hwang, Jae-Woong (Oder Management Team, Environmental Management Corp.) ;
  • Ryu, Dewey D.Y. (Biochemical Engineering Program, Department of Chemical Engineering and Material Science, University of California)
  • Published : 2008.03.31
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Abstract

For the newly isolated $H_2$-producing chemoheterotrophic bacterium Citrobacter amalonaticus Y19, anaerobic glucose metabolism was studied in batch cultivation at varying initial glucose concentrations (3.5-9.5 g/l). The carbon-mass and energy balances were determined and utilized to analyze the carbon metabolic-pathways network. The analyses revealed (a) variable production of major metabolites ($H_2$, ethanol, acetate, lactate, $CO_2$, and cell mass) depending on initial glucose levels; (b) influence of NADH regeneration on the production of acetate, lactate, and ethanol; and (c) influence of the molar production of ATP on the production of biomass. The results reported in this paper suggest how the carbon metabolic pathway(s) should be designed for optimal Hz production, especially at high glucose concentrations, such as by blocking the carbon flux via lactate dehydrogenase from the pyruvate node.

Keywords

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