Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis of the Dimerization of Human CD99 Using Bimolecular Fluorescence Complementation Technique

  • Lee, Mi-Kyung (Department of Biological Sciences, Konkuk University) ;
  • Kim, Hyun-Soo (Department of Biological Sciences, Konkuk University) ;
  • Kim, Seung-Seok (Department of Biological Sciences, Konkuk University) ;
  • Cho, Myung-Hwan (Department of Biological Sciences, Konkuk University) ;
  • Lee, Im-Soon (Department of Biological Sciences, Konkuk University)
  • Published : 2008.03.31
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Abstract

Two isoforms of human CD99 have been identified, but only heterotypic interaction between the isomers was recently demonstrated. In this study, we performed bimolecular fluorescence complementation analysis to further characterize the interaction in vivo. Upon transiently transfecting plasmids expressing either of the two isoforms fused with yellow fluorescent protein (YFP) fragments, all the YFP-tagged CD99 molecules were properly localized on cell surfaces, and formed fluorescent dimers. Interestingly, however, unlike the previous report, the homodimers formed as efficiently as the heterodimer via their extracellular domains, implying its distinct regulatory role through modulating the complex profile.

Keywords

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