Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Effect of Rhamnolipids on Degradation of Anthracene by Two Newly Isolated Strains, Sphingomonas sp. 12A and Pseudomonas sp. 12B

  • Cui, Chang-Zheng (College of Life Sciences, Wuhan University) ;
  • Zeng, Chi (College of Life Sciences, Wuhan University) ;
  • Wan, Xia (College of Life Sciences, Wuhan University) ;
  • Chen, Dong (College of Life Sciences, Wuhan University) ;
  • Zhang, Jia-Yao (College of Resource and Environmental Sciences, Wuhan University) ;
  • Shen, Ping (College of Life Sciences, Wuhan University)
  • Published : 2008.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

Anthracene is a PAH that is not readily degraded, plus its degradation mechanism is still not clear. Thus, two strains of anthracene-degrading bacteria were isolated from long-term petroleum-polluted soil and identified as Sphingomonas sp. 12A and Pseudomonas sp. 12B by a 16S rRNA sequence analysis. To further enhance the anthracene-degrading ability of the two strains, the biosurfactants produced by Pseudomonas aeruginosa $W_3$ were used, which were characterized as rhamnolipids. It was found that these rhamnolipids dramatically increased the solubility of anthracene, and a reverse-phase HPLC assay showed that the anthracene degradation percentage after 18 days with Pseudomonas sp. 12B was significantly enhanced from 34% to 52%. Interestingly, their effect on the degradation by Sphingomonas sp. 12A was much less, from 35% to 39%. Further study revealed that Sphingomonas sp. 12A also degraded the rhamnolipids, which may have hampered the effect of the rhamnolipids on the anthracene degradation.

Keywords

References

  1. Cameotra, S. S. and J. M. Bollag. 2003. Biosurfactant-enhanced bioremediation of polycyclic aromatic hydrocarbons. Crit. Rev. Environ. Sci. Technol. 30: 111-126
  2. Costa, S. G.. V. A. O., M. Nitschke, R. Haddad, M. N. Eberlin, and J. Contiero. 2006. Production of Pseudomonas aeruginosa LBI rhamnolipids following growth on Brazilian native oils. Process Biochem. 41: 483-488 https://doi.org/10.1016/j.procbio.2005.07.002
  3. Dean-Ross, D., J. D. Moody, J. P. Freeman, D. R. Doerge, and C. E. Cerniglia. 2001. Metabolism of anthracene by a Rhodococcus species. FEMS Microbiol Lett. 204: 205-211 https://doi.org/10.1111/j.1574-6968.2001.tb10886.x
  4. Deziel, E., G. Paquette, R. Villemur, F. Lepine, and J. Bisaillon. 1996. Biosurfactant production by a soil Pseudomonas strain growing on polycyclic aromatic hydrocarbons. Appl. Environ. Microbiol. 62: 1908-1912
  5. Fedorak, P. M. and D. Grbic-Galic. 1991. Aerobic microbial cometabolism of benzothiophene and 3-methylbenzothiophene. Appl. Environ. Microbiol. 57: 932-940
  6. Johnsen, A. R. and U. Karlson. 2004. Evaluation of bacterial strategies to promote the bioavailability of polycyclic aromatic hydrocarbons. Appl. Microbiol. Biotechnol. 63: 452-459 https://doi.org/10.1007/s00253-003-1265-z
  7. Johnsen, A. R., L. Y. Wick, and H. Harms. 2005. Principles of microbial PAH-degradation in soil. Environ. Pollut. 133: 71-84 https://doi.org/10.1016/j.envpol.2004.04.015
  8. Kang, Y. S., Y. J. Kim, C. O. Jeon, and W. Park. 2006. Characterization of naphthalene-degrading Pseudomonas species isolated from pollutant-contaminated sites: Oxidative stress during their growth on naphthalene. J. Microbiol. Biotechnol. 16: 1819-1825
  9. Kiyohara, H., K. Nagao, and K. Yana. 1982. Rapid screening of bacteria degrading water-insoluble, solid hydrocarbons on agar plates. Appl. Environ. Microbiol. 43: 454-457
  10. Laha, S. and R. G. Luthy. 1991. Inhibition of phenanthrene mineralization by nonionic surfactants in soil-water systems. Environ. Sci. Technol. 25: 1920-1930 https://doi.org/10.1021/es00023a013
  11. Mata-Sandoval, J. C., J. Karns, and A. Torrents. 1999. High-performance liquid chromatography method for the characterization of rhamnolipid mixtures produced by Pseudomonas aeruginosa UG2 on corn oil. J. Chromatogr. A 864: 211-220 https://doi.org/10.1016/S0021-9673(99)00979-6
  12. Tiehm, A. 1994. Degradation of polycyclic aromatic hydrocarbons in the presence of synthetic surfactants. Appl. Environ. Microbiol. 60: 258-263
  13. Tongpim, S. and M. A. Pickard. 1996. Growth of Rhodococcus S1 on anthracene. Can. J. Microbiol. 42: 289-294 https://doi.org/10.1139/m96-042
  14. Zhang, Y. M., J. M. Walter, and R. M. Maier. 1997. Effect of rhamnolipids on the dissolution, bioavailability, and biodegradation of phenanthrene. Environ. Sci. Technol. 31: 2211-2217 https://doi.org/10.1021/es960687g
  15. Zheng, J. X., J. Y. Zhang, Q. Zhao, L. Zhao, and C. T. Fu. 2006. Bacteria strains for petroleum degrading: Screening culture and UV-induced mutation. Chinese Environ. Sci. Technol. 29: 1-2