Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genes of Rhodobacter sphaeroides 2.4.1 Regulated by Innate Quorum-Sensing Signal, 7,8-cis-N-(Tetradecenoyl) Homoserine Lactone

  • Hwang, Won (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Lee, Ko-Eun (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Lee, Jeong-Kug (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University) ;
  • Park, Byoung-Chul (Translational Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Kun-Soo (Department of Life Science and Interdisciplinary Program of Integrated Biotechnology, Sogang University)
  • Published : 2008.02.29
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Abstract

The free-living photoheterotrophic Gram-negative bacterium Rhodobacter sphaeroides possesses a quorum-sensing (QS) regulatory system mediated by CerR-CerI, a member of the LuxR-LuxI family. To identify the genes affected by the regulatory system, random lacZ fusions were generated in the genome of R. sphaeroides strain 2.4.1 using a promoter-trapping vector, pSG2. About 20,000 clones were screened and 23 showed a significantly different level of ${\beta}$-gal activities upon the addition of synthetic 7,8-cis-N-tetradecenoyl-homoserine lactone (RAI). Among these 23 clones, the clone showing the highest level of induction was selected for further study, where about a ten-fold increase of ${\beta}$-gal activity was exhibited in the presence of RAI and induction was shown to be required for cerR. In this clone, the lacZ reporter was inserted in a putative gene that exhibited a low homology with catD. A genetic analysis showed that the expression of the catD homolog was initiated from a promoter of another gene present upstream of the catD. This upstream gene showed a strong homology with luxR and hence was named qsrR (quorum-sensing regulation regulator). A comparison of the total protein expression profiles for the wild-type cells and qsrR-null mutant cells using two-dimensional gel electrophoresis and a MALDI-TOF analysis allowed the identification of sets of genes modulated by the luxR homolog.

Keywords

References

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