Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Paenibacillus donghaensis sp. nov., a Xylan-degrading and Nitrogen-fixing Bacterium Isolated from East Sea Sediment

  • Choi, Jeong-Hwa (Department of Microbiology and Biotechnology Research Institute, Chungbuk National University) ;
  • Im, Wan-Taek (Department of Biological Sciences, Korea Advanced Institute of Science and technology) ;
  • Yoo, Jae-Soo (Department of School of Electrical and Computer Engineering, Chungbuk National University) ;
  • Lee, Sang-Mahn (Department of Life Science, Chongju University) ;
  • Moon, Deok-Soo (Deep Ocean Water Application Research Center, Korea Ocean research and Development Institute) ;
  • Kim, Hyeon-Ju (Deep Ocean Water Application Research Center, Korea Ocean research and Development Institute) ;
  • Rhee, Sung-Keun (Department of Microbiology and Biotechnology Research Institute, Chungbuk National University) ;
  • Roh, Dong-Hyun (Department of Microbiology and Biotechnology Research Institute, Chungbuk National University)
  • Published : 2008.02.29
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Abstract

A Gram-positive and endospore-forming strain, $JH8^T$, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG $22176^T$ (96.9%). Strain $JH8^T$ was also facultatively anaerobic and grew optimally at $20-25^{\circ}C$. The major cellular fatty acid was anteiso-$C_{15:0}$, and the DNA G+C content was 53.1mol%. The DNA-DNA relatedness between the isolate and Paenibacillus wynnii LMG $22176^T$ was 7.6%, indicating that strain $JH8^T$ and P. wynnii belong to different species. Based on the phylogenetic, phenotypic, and chemotaxonomic characteristics, strain $JH8^T$ would appear to belong to a novel species, for which the name Paenibacillus donghaensis sp. novo is proposed (type strain=KCTC $13049^T=LMG\;237S0^T$).

Keywords

References

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