초록
층꽃나무 지상부위 정유의 화학성분 구명을 위하여 수증기증류법으로 정유를 얻고, 이를 solid-phase microextraction(SPME)와 headspace(HS)법으로 흡착시킨 다음 CC-MS로 분석하였다. 향취가 fougere, woody한 층꽃나무 정유에는 탄화수소 28종, 알코올 22종, 아세테이트 7종, 케톤 7종, 알데히드 3종, 기타 2종 등 총 69종의 화학성분이 함유되어 있었으며, 다함량 성분은 4,6,6-trimethyl [1S-($1{\alpha},2{\beta},5{\alpha}$)]-bicyclo[3.1.1]hept-3-en-2-ol(11.8%), tau-cadinol(9.4%), myrtenyl acetate(9.2%), pinocarvone(7.0%), 1-hydroxy-1,7-dimethy 1-4-isopropyl-2,7-cyclodecadiene(6.3%), ${\delta}$-3-carene(6.2%)이었다. SPME법으로 흡착 후 분석한 시료에는 탄화수소 22종, 알코올 16종, 아세테이트 6종, 케톤 3종, 에테르 2종 등 총 49종의 화학성분이 검출되었는데, 다량 함유된 성분으로는 ${\delta}$-3-carene(12.6%), (-)-myrtenyl acetate(11.2%), 6,6-dimethyl-2-methylene-bicycol[3.1.1] heptan-3-ol(10.9%), pinocarvone(9.3%)이었다. 그리고 HS법으로 흡착한 다음 분석한 정유에는 탄화수소 5종, 아민 2종, 알코올 1종, 기타 2종 등 총 10종의 화학성분이 검출되었는데, 다량 함유된 성분은 (Z)-2-fluoro-2-butene(34.9%), ${\delta}$-3-carene(6.9%), 6-(4-chlorophenul) tetrahydro-2-methyl-2H-1,2-oxazine(5.9%)이었다. HaCaT 각질형성세포에 미치는 세포독성을 조사하기 위하여 MTT assar를 실시한 결과 층꽃나무 정유의 $IC_{50}$값은 0.011 ${\mu}g/mg$으로 시판 중인 로즈마리 정유나 차나무 정유의 $IC_{50}$값보다 낮았는데, 이러한 결과는 층꽃나무 정유의 상업화를 위해서 더 많은 독성시험이 요구된다는 것을 시사한다.
The essential oil was obtained from the aerial part of Caryopteris incana Miq. by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GCMS). The fragrance of the essential oil was fougere and woody. There were sixty-nine constituents in the essential oil: 28 carbohydrates, 22 alcohols, 7 acetates, 7 ketones, 3 aldehydes, and 2 others. Major constituents were 4,6,6-trimethyl [1S-($1{\alpha},2{\beta},5{\alpha}$)]-bicyclo[3.1.1]hept-3-en-2-ol (11.8%), taucadinol (9.4%), myrtenyl acetate (9.2%), pinocarvone (7.0%), 1-hydroxy-1,7-dimethyl-4-isopropyl-2,7-cyclodecadiene (6.3%), ${\delta}$-3-carene (6.2%). By SPME extraction, forty-nine constituents were identified: 22 hydrocarbons, 16 alcohols, 6 acetates, 3 ketones, and 2 ethers. Major constituents of the SPME-extracted sample were ${\delta}$-3-carene (12.6%), (-)-myrtenyl acetate (11.2%), 6,6-dimethyl-2-methylene-bicycol [3.1.1] heptan-3-o1 (10.9%), pinocarvone (9.3%). By HS extraction, ten constituents were identified: 5 hydrocarbons, 2 amines, 1 alcohol, and 2 others. Major constituents of the HS-extracted sample were (Z)-2-fluoro-2-butene (34.9%), ${\delta}$-3-carene (6.9%), 6-(4-chlorophenul)tetrahydro-2-methyl-2H-1,2-oxazine (5.9%). The $IC_{50}$ value (0.011 ${\mu}g/mg$) in MTT assay using HaCaT keratinocyte cell line was lower than those of commercially-selling rosemary and tea tree, suggesting more toxicological studies are needed for commercial use of the essential oil of Caryopteris incana Miq.
