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A Simple and Fast Web Alignment Tool for Large Amount of Sequence Data

  • Lee, Yong-Seok (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Jeong-Su (Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2008.09.30

Abstract

Multiple sequence alignment (MSA) is the most important step for many of biological sequence analyses, homology search, and protein structural assignments. However, large amount of data make biologists difficult to perform MSA analyses and it requires much computational time to align many sequences. Here, we have developed a simple and fast web alignment tool for aligning, editing, and visualizing large amount of sequence data. We used a cluster server installed ClustalW-MPI using web services and message passing interface (MPI). It also enables users to edit multiple sequence alignments for manual editing and to download the input data and results such as alignments and phylogenetic tree.

Keywords

References

  1. Ebedes, J., and Datta, A. (2004). Multiple sequence alignment in parallel on a workstation cluster. Bioinformatics 20, 1193-1195 https://doi.org/10.1093/bioinformatics/bth055
  2. Kleinjung, J., Douglas, N., and Heringa, J. (2002). Parallelized multiple alignment. Bioinformatics 18, 1270-1271 https://doi.org/10.1093/bioinformatics/18.9.1270
  3. Li, K.B. (2003). ClustalW-MPI: ClustalW analysis using distributed and parallel computing. Bioinformatics 19, 1585-1586 https://doi.org/10.1093/bioinformatics/btg192
  4. Mikhailov, D., Cofer, H., and Gomperts, R. (2001). Performance optimization of ClustalW: parallel ClustalW, HT Clustal, and MULTICLUSTAL. White papers, Silicon Graphics, Mountain View, CA
  5. Shin, J., Park, H., Ahn, Y., Cho, D., Kim, J., Kee, M., Kim, S., Lee, J., and Kim, S. (2008). GTVseq: a web-based genotyping tool for viral sequences. Genomics & Informatics 6, 54-56 https://doi.org/10.5808/GI.2008.6.1.054
  6. Thompson, J.D., Higgins, D.G., and Gibson, T.J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680 https://doi.org/10.1093/nar/22.22.4673
  7. Woo, T., Kim, Y., Kwon, J., and Seo, J. (2007). RepWeb: a web-based search tool for repeat-related literatures. Genomics & Informatics 5, 89-91
  8. CodeIgniter (2008). http://www.coldscripts.com/
  9. XFIRE site. (2008). http://xfire.codehaus.org/
  10. Rocksclusters site. (2008). http://www.rocksclusters.org
  11. MPI site. (2008). http://www-unix.mcs.anl.gov/mpi/