YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Protective Effect of Oenanthe javanica Extract on Acetaminophen-induced Hepatotoxicity in Rats

Acetaminophen으로 유도한 쥐의 간 독성에 대한 미나리(Oenanthe javanica) 추출액의 간 보호 작용

  • Published : 2008.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

The hepatoprotection by the methanol extract of Oenanthe javanica DC (water dropwort) (OJME) was investigated in Sprague Dawley rats with inducing liver damage by acetaminophen. After OJME administration for 1 week, the increase of hepatic lipid peroxide level by acetaminophen-induced hepatotoxicity was significantly reduced. In case of phase I microsomal enzyme systems including cytochrome P-450, aminopyrine N-demethylase and aniline hydroxylase, any significant differences between in control and in OJME-pretreated group was observed after acetaminophen treatment. However, the pretreatment of OJME maintained the hepatic glutathione level and the activity of liver cytosolic glutathione S-transferase, which was significantly decreased by the acetaminophen intoxication. Among the glutathione-generating system, glutathione reductase was more responsible for its biosynthesis rather than ${\gamma}-glutamylcystein$ synthetase. OJME itself showed the strong inhibition activity on DPPH radical generation. In conclusion, OJME administration maintains the liver glutathione pool and hepatic glutathione S-transferase activity, in addition with its high anti-oxidative capability, to show hepatoprotective effect from acetaminophen intoxication.

Keywords

References

  1. Menon, K. V., Gores, G. J. and Shah, V. H. : Pathogenesis, diagnosis, and treatment of alcoholic liver disease. Mayo Clin. Proc. 76, 1021 (2001)
  2. Stickel, F., Hoehn, B., Schuppan, D. and Seitz, H. K. : Nutritional therapy in alcohol liver damage. Aliment. Pharmacol. Ther. 18, 357 (2003) https://doi.org/10.1046/j.1365-2036.2003.01660.x
  3. Calabrese, V., Bombaci, G., Calderone, A. and Rizza, V. : Effects of metadoxine on cellular free fatty acid levels in ethanol treated rats. Int. J. Tissue React. 15, 235 (1993)
  4. Calabrese, V., Calderone, A., Ragusa, N. and Rizza, V. : Effects of metadoxine on cellular status of glutathione and of enzymatic defence system following acute ethanol intoxication in rats. Drugs Exp. Clin. Res. 22, 17 (1996)
  5. Park, J. C., Young, H. S., Yu, Y. B. and Lee, J. H. : Isorhamnetin sulphate from the leaves and stems of Oenanthe javanica in Korea. Planta Med. 61, 377 (1995) https://doi.org/10.1055/s-2006-958110
  6. Park, J. C., Yu, Y. B., Lee, J. H., Hattori, M., Lee, C. K. and Choi, J. W. : Protective effect of Oenanthe javanica on the hepatic lipid peroxidation in bromobenzene-treated rats and its bioactive component. Planta Med. 62, 488 (1996) https://doi.org/10.1055/s-2006-957954
  7. Park, J. C. and Choi, J. W. : Effects of methanol extract of Oenanthe javanica on the hepatic alcohol-metabolizing enzyme system and its bioactive component. Phytother. Res. 11, 260 (1997) https://doi.org/10.1002/(SICI)1099-1573(199705)11:3<260::AID-PTR80>3.0.CO;2-N
  8. Ohkawa, H., Ohishi, N. and Yaki, K. : Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351 (1979) https://doi.org/10.1016/0003-2697(79)90738-3
  9. Omura, T. and Sato, R. : The carbon monooxide binding pigments of liver microsomes. I. Evidence for its hemoprotein nature. J. Biol. Chem. 239, 2370 (1964)
  10. Nash, T. : The colorimetric estimation of formaldehyde by means of the Hentisch reaction. J. Biol. Chem. 55, 412 (1953)
  11. Bidlack, W. R. and Lowery, G. L. : Multiple drug metabolism: p-nitro-anisole reversal of acetone enhanced aniline hydroxylation. Biochem. Pharmacol. 31, 311 (1982) https://doi.org/10.1016/0006-2952(82)90176-9
  12. Habig, W. H., Pabist, M. J. and Jakoby, W. B. : The first step in mercapturic acid formation. J. Biol. Chem. 249, 7130 (1974)
  13. Meister, A. and Richman, P. G. : Regulation of $\gamma$-glutamylcysteine synthesis by nonallosteric feedback inhibition by glutathione. J. Biol. Chem. 250, 1422 (1975)
  14. Mize, C. E. and Langdon, R. G. : Hepatic glutathione reductase. I. Purification and general kinetic proterties. J. Biol. Chem. 237, 1589 (1962)
  15. Boyne, A. F. and Ellman, G. L. : A methodology for analysis of tissue sulfhydryl components. Anal. Biochem. 46, 639 (1972) https://doi.org/10.1016/0003-2697(72)90335-1
  16. Hatano, T., Edamatsu, R., Hiramatsu, M., Mori, A., Fujita, Y., Yasuhara, T. and Okuda, T. : Effects of the interaction of tannins with co-existing substance. VI. Effects of tannins and related polyphenols and superoxide anion radical, and on 1,1- diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016 (1989) https://doi.org/10.1248/cpb.37.2016
  17. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randal, R. J. : Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265 (1951)
  18. McClain, C. J., Price, S., Barve, S., Devalarja, R. and Shedlofsky, S. : Acetaminophen hepatotoxicity: an update. Curr. Gastroenterol. Rep. 1, 42 (1999) https://doi.org/10.1007/s11894-999-0086-3
  19. Schiodt, F. V., Rochling, F. A., Casey, D. L. and Lee, W. M. : Acetaminophen toxicity in an urban county hospital. New Engl. J. Med. 337, 1112 (1997) https://doi.org/10.1056/NEJM199710163371602
  20. McClain, C. J. : Clinical features of acetaminophen toxicity. J. Clin. Gastroenterol. 10, 76 (1988) https://doi.org/10.1097/00004836-198802000-00016