Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Haepyoejin-tang Plus Antler in Formalin-Injected Chronic Inflammation Model in Mice

해표이진탕가녹용(解表二陳湯加鹿茸)이 Formalin으로 유발된 마우스의 만성 염증에 미치는 영향

  • Liu, Han-Hsiang (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Choi, Hae-Yun (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Park, Mee-Yeon (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Naam, Yee-Hyun (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University) ;
  • Kim, Jong-Dae (Department of Internal Medicine, College of Oriental Medicine, Daegu Haany University)
  • 유한상 (대구한의대학교 한의과대학 내과학교실) ;
  • 최해윤 (대구한의대학교 한의과대학 내과학교실) ;
  • 박미연 (대구한의대학교 한의과대학 내과학교실) ;
  • 남이현 (대구한의대학교 한의과대학 내과학교실) ;
  • 김종대 (대구한의대학교 한의과대학 내과학교실)
  • Published : 2008.06.25
Download PDF
⟨ Previous Next ⟩

Abstract

In the present study, the anti-inflammatory effect of Haepyoejin-tang plus antler water extract was tested in formalin-injected chronic inflammation model in mouse hind paw. The test articles-100, 250, 500 mg/kg- were dosed once a day for 10 days, and changes on the body weight, paw weights were observed with histopathology of induced paw dorsum pedis. In addition, histomorphometry was also monitored at sacrifice. 15 mg/kg/10 mL of dexamethasone (DEXA) and diclofenac (DICLO) intraperitoneally dosed groups were used as reference groups. A significantly decrease of both absolute and relative paw weights were observed in all dosing groups including DEXA and DICLO groups compared to that of control, and a significantly decrease of the differences between intact and induced paw weights were also observed Haepyoejin-tang plus antler dosing groups compared to that of control. These histological signs-hypertrophy of paw dorsum pedis tissues were detected results from edematous changes on the cutaneous and subcutaneous tissues with severe infiltration of chronic inflammatory cells-were dramatically decreased in all dosing groups including DEXA and DICLO dosing groups compared to that of control. Especially, dose dependently decreases were detected in Haepyoejin-tang plus antler dosing groups compared to that of control. Base on these aforementioned results, it is concluded that Haepyoejin-tang plus antler have clear anti-inflammatory effect on the chronic inflammation induced by formalin injection.

Keywords

References

  1. 賣得道. 中國醫學史略. 山西, 山西人民出版社, p 243, 1979
  2. 전국한의과대학 폐계내과학교실편저. 東醫肺系內科學. 서울, 도서출판 국진기획, p 192, 199, 320, 575, 589, 2004
  3. 허준. 東醫寶鑑. 서울, 남산당, p 134, 481, 1987
  4. 신재용. 방약합편해설. 서울, 성보사, p 133, 1998
  5. 대한내과학회 편. Harrison's 내과학(16판). 서울, 도서출판 MIP, pp 1645-1646, 2006
  6. 김민수, 박동일. 加味解表二陳湯이 알레르기 喘息 白鼠의 呼吸 양상과 기관 조직에 미치는 영향. 동의생리병리학회지 16(3):557-562, 2002
  7. 백동진, 정희재, 이형구, 정승기. 解表二陳湯加減方이 천식 모델 내의 Cytokine에 미치는 영향. 대한한의학회지 21(3): 57-67, 2000
  8. 정봉균, 전귀옥, 조동희, 박미연, 최해윤, 김종대. 解表二陳湯加鹿茸이 Xylene로 유발된 마우스의 급성 염증에 미치는 영향. 동의생리병리학회지 20(4):985-991, 2006
  9. Hosseinzadeh, H., addadkhodaparast, M.H., Arash, A.R. Antinociceptive, anti-inflammatory and acute toxicity effects of Salvia leriifolia Benth seed extract in mice and rats, Phytother Res, 17(4):422-425, 2003 https://doi.org/10.1002/ptr.1154
  10. 대한병리학회 편. 병리학(제5판). 서울, 고문사, p 68, 84, 2005
  11. 대한병리학회 대구경북지부학회. 간추린 병리학(2판). 서울, 정문각, pp 63, 73-77, 2000
  12. 고려대학교 의과대학 병리학교실 편. 병리학. 서울, 신광출판사, pp 25-33, 2001
  13. 노영득, 심영섭, 송호준, 신민교. 牧丹皮의 항염효과에 관한 연구. 대한본초학회지 19(3):13-24, 2004
  14. 조형준, 이진용, 김덕곤. 桑白皮가 항알러지 및 항염증반응에 미치는 영향. 한방소아과학회지 19(2):175-195, 2005
  15. 김석근, 송호준. 蒲公英 수추출물이 진통 항염작용에 미치는 영향. 대한한의학회지 13(1):152-161, 1992
  16. 손대범, 송성필, 황치환. 大黃, 黃芩, 黃柏 복합 추출물의 항균, 항염 및 항알레르기 효과에 대한 실험적 연구. 한방안이비인후피부과학회지 18(1):154-171, 2005
  17. 강승원, 노석선. 回春凉膈散과 龍石散이 항염작용에 미치는 영향. 한방외관과학회지 12(1):47-78, 1992
  18. 강호건, 최병태, 김영희, 강호성, 김한도, 고우신. 옹저에 사용되는 托裏消毒飮의 항염증효과. 대한한의학회지 21(1):45-52, 2002
  19. 임종필, 최훈. 排膿散이 프로테이나아제 활성수용체-2에 의한 흰쥐 발바닥 부종에 미치는 항염효과. 동의생리병리학회지 18(1):110-113, 2004
  20. 송무식, 조동희, 전귀옥, 박미연, 최해윤, 김종대. 定喘化痰降氣湯이 Xylene으로 유발된 마우스의 급성 염증에 미치는 영향. 동의생리병리학회지 20(4):875-881, 2006
  21. 신창호, 김종대, 최해윤, 박미연. 定喘化痰降氣湯이 Formalin으로 유발된 마우스의 만성 염증에 미치는 영향. 동의생리병리학회지 21(6):1470-1476, 2007
  22. Abdel-Salam, O.M., Baiuomy, A.R., El-batran, S., Arbid, M.S. Evaluation of the anti-inflammatory, anti-nociceptive and gastric effects of Ginkgo biloba in the rat. Pharmacol Res, 49(2):133-142, 2004 https://doi.org/10.1016/j.phrs.2003.08.004
  23. Pillai, A.D., Rathod, P.D., P. X. F., Patel, M., Nivsarkar, M., Vasu, K.K. et al. Novel drug designing approach for dual inhibitors as anti-inflammatory agents: implication of pyridine template. Biochem Biophys Res Commun 301(1):183-186, 2003 https://doi.org/10.1016/S0006-291X(02)02996-0
  24. Hosseinzadeh, H., Younesi, H.M. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. BMC Pharmacol 15(2):7, 2002
  25. Torres-Lopez, J.E., Ortiz, M.I., Castaneda-Hernandez, G., Alonso-Lopez, R., Asomoza-Espinosa R., Granados-Soto V. Comparison of the antinociceptive effect of celecoxib, diclofenac and resveratrol in the formalin test. Life Sci 70(14):1669-1676, 2002 https://doi.org/10.1016/S0024-3205(02)01491-1
  26. Gupta, S.K., Bansal, P., Bhardwaj, R.K., Jaiswal, J. Velpandian T. Comparison of analgesic and anti-inflammatory activity of meloxicam gel with diclofenac and piroxicam gels in animal models: pharmacokinetic parameters after topical application. Skin Pharmacol Appl Skin Physiol 15(2):105-111, 2002 https://doi.org/10.1159/000049397
  27. Yashpal K., Coderre T.J. Influence of formalin concentration on the antinociceptive effects of anti-inflammatory drugs in the formalin test in rats: separate mechanisms underlying the nociceptive effects of low and high-concentration formalin. Eur J Pain 2(1):63-68, 1998 https://doi.org/10.1016/S1090-3801(98)90047-7
  28. Moura, A.C., Silva, E.L., Fraga, M.C., Wanderley, A.G., Afiatpour, P., Maia, M.B. Antiinflammatory and chronic toxicity study of the leaves of Ageratum conyzoides L. in rats. Phytomedicine 12(1-2):138-142, 2005 https://doi.org/10.1016/j.phymed.2003.12.003
  29. Dharmasiri, M.G., Jayakody, J.R., Galhena, G., Liyanage, S.S., Ratnasooriya, W.D. Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J Ethnopharmacol 87(2-3):199-206, 2003 https://doi.org/10.1016/S0378-8741(03)00159-4
  30. Lakshmi, B., Ajith, T.A., Sheena, N., Gunapalan, N., Janardhanan, K.K. Antiperoxidative, anti-inflammatory, and antimutagenic activities of ethanol extract of the mycelium of Ganoderma lucidum occurring in South India. Teratog Carcinog Mutagen 1: 85-97, 2003
  31. Pillai, A.D., Rathod, P.D., P X F, Patel, M., Nivsarkar, M., Vasu, K.K. et al. Novel drug designing approach for dual inhibitors as anti-inflammatory agents: implication of pyridine template. Biochem Biophys Res Commun 301(1):183-186, 2003 https://doi.org/10.1016/S0006-291X(02)02996-0
  32. Ahmadiani, A., Hosseiny, J., Semnanian, S., Javan, M., Saeedi, F., Kamalinejad, M. et al. Antinociceptive and anti-inflammatory effects of Elaeagnus angustifolia fruit extract. J Ethnopharmacol 72(1-2):287-292, 2000 https://doi.org/10.1016/S0378-8741(00)00222-1
  33. 전국한의과대학 본초학교수 편저. 본초학. 서울, 영림사, p 121, 125, 136, 460, 463, 478, 481, 484, 540, 2000
  34. Lockshin, M.D., Sammaritano, L.R. Corticosteroids during pregnancy. Scand J Rheumatol Suppl 107: 136-138, 1998
  35. Waddel,l A.N., Patel, S.K., Toma, A.G., Maw, A.R. Intranasal steroid sprays in the treatment of rhinitis: is one better than another. J Laryngol Otol 117(11):843-845, 2003
  36. Roh, G.S., Seo, S.W,, Yeo, S., Lee, J.M., Choi, J.W., Kim, E. et al. Efficacy of a traditional Korean medicine, Chung-Sang-Bo-Ha-Tang, in a murine model of chronic asthma. Int Immunopharmacol 5(2):427-436, 2005 https://doi.org/10.1016/j.intimp.2004.09.036
  37. Leclerc, N., Noh, T., Khokhar, A., Smith, E., Frenkel, B. Glucocorticoids inhibit osteocalcin transcription in osteoblasts by suppressing Egr2/Krox20-binding enhancer. Arthritis Rheum 52(3):929-939, 2005 https://doi.org/10.1002/art.20872
  38. Kenjo, T., Kikuchi, S., Konno, S. Cooling decreases fos-immunoreactivity in the rat after formalin injection. Clin Orthop Relat Res 394: 271-277, 2002 https://doi.org/10.1097/00003086-200201000-00032
  39. Dumka, V.K., Tandan, S.K., Raviprakash, V., Tripathi, H.C. Central noradrenergic and cholinergic modulation of formaldehyde-induced pedal inflammationand nociception in rats. Indian J Physiol Pharmaco 40(1):41-46, 1996
  40. Perez, R.M., Perez, S., Zavala, M.A., Salazar, M. Anti- inflammatory activity of the bark of Hippocratea excelsa. J Ethnopharmacol 47(2):85-90, 1995 https://doi.org/10.1016/0378-8741(95)01257-E