Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Scutellaria baicalensis Georgi Extracts inhibit RANKL-induced Osteoclast Differentiation

  • Shim, Ki-Shuk (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology) ;
  • Kim, Soon-Nam (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology) ;
  • Kim, Myung-Hee (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology, Department of Biochemistry, Chungnam National University) ;
  • Kim, Young-Sup (Laboratory of Phytochemistry Research, Korea Research Institute of Chemical Technology) ;
  • Ryu, Shi-Yong (Laboratory of Phytochemistry Research, Korea Research Institute of Chemical Technology) ;
  • Min, Yong-Ki (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology) ;
  • Kim, Seong-Hwan (Laboratory of Chemical Genomics, Korea Research Institute of Chemical Technology)
  • Published : 2008.09.30
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Abstract

Scutellaria baicalensis Georgi (SBG) is traditionally used medicinal herb that has anti-oxidant, anticancer and anti-inflammatory effects. In this study, we investigated whether the extracts of SBG have the inhibitory activity in the osteoclast differentiation by using mouse monocytes RAW264.7 cells and primary mouse bone marrow-derived macrophages (BMMs). Methanol extract (ME) from SBG was successively fractionated into methylene chloride (MF), ethylacetate (EF) and n-butanol fraction (BF). The activity assay for tartrateresistant acid phosphatase (TRAP) and Western blot analysis were employed to evaluate the osteoclasts differentiation and the activation of mitogen-activated protein (MAP) kinases, respectively. ME, MF, EF and BF significantly and dose-dependently inhibited osteoclast differentiation without the decrease of cell viability at the concentrations used in this study. In addition, ME significantly inhibited the activation of c-jun-N-terminal kinase (JNK). In conclusion, this study firstly demonstrated that ME of SBG has the potential to inhibit the osteoclast differentiation through the suppression of JNK activation partially.

Keywords

References

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