The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Influence of Five Herbal Medicines on Cytochrome P450 3A4 Drug-Metabolizing Enzyme Activity

활혈거어약의 Cytochrome P450 3A4 효소활성에 미치는 영향

  • Go, Jae-Eon (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Hwang, Jin-Woo (Department of Oriental internal Medicine, National Medical Center) ;
  • Go, Ho-Yeon (Department of internal Medicine, College of Oriental Medicine, Se-Myung University) ;
  • Choi, You-Kyung (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Park, Jong-Hyung (Department of internal Medicine, College of Oriental Medicine, Kyungwon University) ;
  • Ko, Seong-Gyu (Department of Preventive Medicine, College of Oriental Medicine, Kyunghee University) ;
  • Jun, Chan-Yong (Department of internal Medicine, College of Oriental Medicine, Kyungwon University)
  • 고재언 (경원대학교 한의과대학 내과학교실) ;
  • 황진우 (국립의료원 한방진료부 한방내과) ;
  • 고호연 (세명대학교 한의과대학 내과학교실) ;
  • 최유경 (경원대학교 한의과대학 내과학교실) ;
  • 박종형 (경원대학교 한의과대학 내과학교실) ;
  • 고성규 (경희대학교 한의과대학 예방의학교실) ;
  • 전찬용 (경원대학교 한의과대학 내과학교실)
  • Published : 2008.09.30
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Abstract

Objectives: The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 (CYP) 3A4 drug-metabolizing enzymes in human liver microsomes. Methods: By using of human liver microsomes, we extracted Cnidium officinale Makino, Rhus verniciflua Stokes, Prunus persica Batsch, Corydalis remota Fisch, Carthamus tinctorius Linne, which are called Hwalhyulgeoouhyak(活血祛瘀藥). Then they were incubated and measured for relative enzyme activity under incubation conditions compared to ketoconazole, which is known as a representative inhibitor of CYP 3A4. Results: We showed that all of five traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and 50${\mu}g/m{\ell}$ doses in human liver microsomes, although Rhus verniciflua Stokes (RVS) showed a little inhibition as about 95% enzyme activity of control. However, this result was not enough to prove that RVS has a CYP 3A4 inhibition effect. Moreover, we can't confirm that those rates have significant induction effect on CYP 3A4. Conclusions: The result of this study could support that those herbal medicines are more reliable than chemical drugs, even if this is a basic step to prove that result.

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