Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Interactive Effects of Ozone and Light Intensity on Platanus occidentalis L. Seedlings

  • Kim, Du-Hyun (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Han, Sim-Hee (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Kab-Yeon (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Pan-Gi (Division of Forest Environment and Resources, Kyungpook National University)
  • Received : 2008.05.26
  • Accepted : 2008.06.19
  • Published : 2008.10.31
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Abstract

Sycamore (Platanus occidentalis L.) seedlings were grown under low light intensity and ozone treatments to investigate the role of the light environment in their response to chronic ozone stress. One-year-old seedlings of Platanus occidentalis L. were grown in pots for 3 weeks under low light (OL, $150{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and high light (OH, $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) irradiance in combination with 150 ppb of ozone fumigation. After three weeks of ozone and light treatment, seedlings were placed in ozone free clean chamber for 3 weeks for recovery from ozone stress with same light conditions to compare recovery capacity. Ozone fumigation determined an impairment of the photosynthetic process. Reduction of leaf dry weight (14%) and shoo/root ratio (17%) were observed in OH treatment. OL treatment also showed severe reductions in leaf dry weight and shoot/root ratio by 48% and 36% comparing to control, respectively. At the recovery phase, OH-treated plants recovered their biomass, whereas OL-treated plant showed reduction in leaf dry weight (52%) and shoot/root ratio (49%). OH-treated plants reached similar relative growth rate (RGR) comparing to control, whereas OL-treated plants showed lower RGR in stem height. However, there were no significant differences in response to those treatments in stem diameter RGR at the recovery phase. Ozone treatment produced significant reduction of net photosynthesis in both high and low light treatments. Carboxylation efficiency and apparent quantum yield in OL-treated plants showed significant reductions rate to 10% and 45%, respectively. At the recovery stage, ozone exposed seedlings under high light had similar photosynthetic capacity comparing to control plants. Antioxidant enzymes activities such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR) were increased in ozone fumigated plants only under low light. The present work shows that the physiological changes occur in photosynthesis-related parameters and growth due to ozone and low light stress. Thus, low light seems to enhance the detrimental effects of ozone on growth, photosynthesis, and antioxidant enzyme responses.

Keywords

References

  1. Beauchamp, C. and Fridovichi, I. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44: 276-297. https://doi.org/10.1016/0003-2697(71)90370-8
  2. Carlberg, I. and Mannervik, B. 1985. Glutathione reductase. Methods in Enzymology 113: 485-490.
  3. Chappelka, A.H. and Samuelson, L.J. 1998. Ambient ozone effects on forest trees of the eastern United States: a review. New Phytologist 139: 91-108. https://doi.org/10.1046/j.1469-8137.1998.00166.x
  4. Demmig-Adams B. and Adams, W.W. 1992. Photoprotection and other responses of plants to high light stress. Annual Review of Plant Physiology and Plant Molecular Biology 43: 599-626. https://doi.org/10.1146/annurev.pp.43.060192.003123
  5. Dizengremel, P. 2001. Effects of ozone on the carbon metabolism of forest trees. Plant Physiology and Biochemistry 39: 729-742. https://doi.org/10.1016/S0981-9428(01)01291-8
  6. Farquhar, G.D., von Caemmer, S. and Berry, J.A. 1980. A biochemical model of photosynthetic $CO_2$ assimilation in leaves of $C_3$ species. Planta 149: 78-90. https://doi.org/10.1007/BF00386231
  7. Fossati, P., Prencipe, L. and Berti, G. 1980. Use of 3,5- dichloro-2-hydroxy benzenesulfonic acid /4-aminophenazone chromogenic system in direct enzyme assay of uric acid in serum and urine. The Clinical Chemistry Methodology 26: 227-231.
  8. Fredericksen, T.S., Kolb, T.E., Skelly, J.M., Steiner, K.C., Joyce, B.J. and Savage, J.E. 1996. Light environment alters ozone uptake per net photosynthetic rate in black cherry (Prunus serotina Ehrh.) trees. Tree Physiology 16: 458-490.
  9. Han, S.H., Kim, D.H., Lee, K.Y., Ku J.J. and Kim P.G. 2007. Physiological damages and biochemical alleviation to ozone toxicity in five species of genus Acer. Journal of Korea Forestry 96: 551-560.
  10. Han, S.H., Lee, J.C., Lee, W.Y., Park, Y. and Oh C.Y. 2006. Antioxidant characteristics and phytoremediation potential of 27 texa of roadside trees at industrial complex area. Korean Horticultural of Agricultural and Forest Meteorology 8: 159-168.
  11. Kangasjärvi, J., Jaspers, P. and Kollist, H. 2005. Signalling and cell death in ozone-exposed plants. Plant, Cell and Environment 28: 1021-1036. https://doi.org/10.1111/j.1365-3040.2005.01325.x
  12. Karnosky, D.F., Skelly, J.M., Percy, K.E. and Chappelka, A.H. 2007. Perspectives regarding 50 years of research on effects of tropospheric ozone air pollution on US forests. Environmental Pollution 147: 489-506. https://doi.org/10.1016/j.envpol.2006.08.043
  13. Kim, P.G. and Lee, E.J. 2001. Ecophysiology of photosynthesis 1: Effects of light intensity and intercellular $CO_2$ pressure on photosynthesis. Korean Journal of Agricultural and Forest Meteorology 3: 126-133.
  14. Laurence, J.A., Kohut, R.G., Amundson, R.G., Weinstein, D.A. and Maclean, D.C. 1996. Response of sugar maple to ozone and simulated acid precipitation. Environmental Pollution 92: 119-126. https://doi.org/10.1016/0269-7491(95)00105-0
  15. Lee, J.C., Han, S.H., Kim, P.G., Jang, S.S. and Woo, S.Y. 2003. Growth, physiological responses and ozone uptake of five Betula species exposed to ozone. Korean Journal of Ecology 26: 165-172. https://doi.org/10.5141/JEFB.2003.26.4.165
  16. Lee, J.C., Oh, C.H., Han, S.H. and Kim, P.G. 2005. Changes on photosynthesis and SOD Activity in Platanus orientalis and Liriodendron tulipifera according to ozone exposing period. Korean Journal of Agricultural and Forest Meteorology 7: 156-163.
  17. Lee, Y.K., Kim, S.M. and Han, S.W. 2003. Ozoneinduced inactivation of antioxidant enzymes. Biochimie 85: 947-952. https://doi.org/10.1016/j.biochi.2003.09.012
  18. Matyssek, R., Bytnerowicz, A., Karlsson, P.E., Paoletti, E., Sanz, M., Schaub, M. and Wieser, G. 2007. Promoting the $O_3$ flux concept for European forest tree. Environmental Pollution 146: 587-607. https://doi.org/10.1016/j.envpol.2006.11.011
  19. Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880.
  20. Neufeld H.S., Renfro, J.R., Hacker, W.D. and Silsbee, D. 1992. Ozone in Great Smoky Mountain National Park: dynamics and effects on plants. pp. 594-617. In: Berglund R.L. (Ed.), Transactions: Tropospheric Ozone and the Environment II. Pittsburgh, PA, USA: Air and Waster Management Association.
  21. Novak K., Schaub, M., Fuhrer, J., Skelly, J.M., Hug, C., Landolt, W., Bleuler, P. and Krauchi, N. 2005. Seasonal trends in reduced leaf gas exchange and ozone induced foliar injury of three ozone sensitive woody plant species. Environmental Pollution 136: 33-45. https://doi.org/10.1016/j.envpol.2004.12.018
  22. Novak K., Schaub, M., Fuhrer, J., Skelly, J.M., Frey, B. and Kräuchi, N. 2008. Ozone effects on visible foliar injury and growth of Fagus sylvatica and Viburnum lantana seedlings grown in monoculture or in mixture. Environmental and Experimental Botany 62: 212-220. https://doi.org/10.1016/j.envexpbot.2007.08.008
  23. Nunn, A.J., Reiter, I.M., Häberle, K.H., Langebartels, C.H., Bahnweg, G., Pretzch, H.,Sandermann, H. Jr. and Matyssek, R. 2005. Response patterns in adult forest trees to chronic ozone stress: identification of variations and consistencies. Environmental Pollution 136: 365-369. https://doi.org/10.1016/j.envpol.2005.01.024
  24. Paludan-Muller, G., Saxe, H. and Leverenz, J.W. 1999. Responses to ozone in 12 provenances of European beech (Fagus sylvatica): genotypic variation and chamber effects on photosynthesis and dry matter partitioning. New Phytologist 144: 261-273. https://doi.org/10.1046/j.1469-8137.1999.00518.x
  25. Paoletti, E., Bytnerowicz, A. and Andersen, C. 2007. Impacts of air pollution and climate change on forest ecosystems-emerging research needs. The Scientific World Journal 7: 1-8.
  26. Paoletti, E., Nali, C., Marabottini, R., Della Rocca, G., Lorenzini, G., Paolacci, A.R., Ciaffi, M. and Badiani, M. 2003. Strategies of response to ozone in Mediterranean evergreen species pp. 336-343. In: Karlsson, P.E., Sellden, G. and Pleijel, H. (Eds.), Establishing Ozone Critical Levels II. UNECE Workshop Report. IVL report B 1523, IVL Swedish Environmental Research Institute, Göteborg, Sweden.
  27. Rebbeck, J. and Scherzer, A.J. 2002. Growth responses of yellow-poplar (Liriondendrn tulipifera L.) exposed to 5 years of $O_3$ alone or combined with elevated $CO_2$. Plant, Cell and Environment 25: 1527-1537. https://doi.org/10.1046/j.1365-3040.2002.00933.x
  28. Samuelson, L.J. 1994 a. The role of microclimate in determining the sensitivity of Quercus rubra L. to ozone. New Phytologist 128: 235-241. https://doi.org/10.1111/j.1469-8137.1994.tb04007.x
  29. Samuelson, L.J. 1994 b. Ozone-exposure responses of black cherry and red maple seedlings. Environmental and Experimental Botany 34: 355-362. https://doi.org/10.1016/0098-8472(94)90017-5
  30. Tjoelker, M.G., Volin, J.C., Oleksyn, J. and Reich, P.B. 1993. Light Environment alters response to ozone stress in seedlings of Acer saccharum Marsh. and hybrid Populus L. I. In situ net photosynthesis, dark respiration and growth. New Phytologist 124: 627-636. https://doi.org/10.1111/j.1469-8137.1993.tb03852.x
  31. Tjoelker, M.G., Volin, J.C., Oleksyn, J. and Reich, P.B. 1995. Interaction of ozone pollution and light effects on photosynthesis in a forest canopy experiment. Plant, Cell and Environment 18: 895-905. https://doi.org/10.1111/j.1365-3040.1995.tb00598.x
  32. Topa, M.A., Vanderklein, D.W. and Corbin, A. 2001. Effects of elevated ozone and low light on diurnal and seasonal carbon gain in sugar maple. Plant, Cell and Environment 24: 663-677. https://doi.org/10.1046/j.0016-8025.2001.00722.x
  33. Topa, M.A., McDermitt, D.J., Yun, S.C. and King, P.S. 2004. Do elevated ozone and variable light alter carbon transport to roots in sugar maple? New Phytologist 162: 173-186. https://doi.org/10.1111/j.1469-8137.2004.01014.x
  34. Wei, C., Skelly, J.M., Pennypacker, S.P., Ferdinand, J.A., Savage, J.E., Stevenson, R.E. and Davis, D.D. 2004. Influence of light fleck and of light on foliar injury and physiological responses of two hybrid poplar clones to ozone. Environmental Pollution 130: 215-227. https://doi.org/10.1016/j.envpol.2003.12.009
  35. Wieser G, Havranek, W.M., Loidolt-Nagele, M., KronfuB, G. and Polle, A. 1998. Response of photosynthesis, carbohydrates and antioxidants in needles of Norway spruce to slow and rapid changes in ozone. Botanica Acta 111: 35-41. https://doi.org/10.1111/j.1438-8677.1998.tb00674.x
  36. Wieser, G. and Matyssek, R. 2007. Linking ozone uptake and defense towards a mechanistic risk assessment for forest trees. New Phytologist 174: 7-9. https://doi.org/10.1111/j.1469-8137.2007.01994.x
  37. Wieser, G., Tegischer, K., Tausz, M., Häberle, K.H., Grams, T.E.E., and Matyssek, R. 2002. Age effects on Norway spruce (Picea abies) susceptibility to ozone uptake: a novel approach relating stress avoidance to defense. Tree Physiology 22: 583-590. https://doi.org/10.1093/treephys/22.8.583
  38. Witting, V. E., Elizabeth, A. and Long, S.P. 2007. To what extent do current and projected increases in surface ozone affect photosynthesis and stomatal conductance of trees? A meta-analytic review of the last 3 decades of experiments. Plant, Cell and Environment 30: 1150-1162. https://doi.org/10.1111/j.1365-3040.2007.01717.x
  39. Woo, S.Y. 2006. Trends of several air pollutants and the effects of ozone on the plant antioxidant system in Platanus occidentalis in Korea. Journal of Korean Forestry 95: 183-187.
  40. Zhou, J.F., Cai, D. and Tong, G.Z. 2003. Oxidative stress and potential free radical damage associated with photocopying. A role for ozone? Free Radical Research 37: 137-143. https://doi.org/10.1080/1071576021000036623